Theranostics 2015; 5(1):71-85. doi:10.7150/thno.10117 This issue Cite

Research Paper

Copy Number Variation Analysis by Ligation-Dependent PCR Based on Magnetic Nanoparticles and Chemiluminescence

Ming Liu1, Ping Hu2, Gen Zhang1,3, Yu Zeng2, Haowen Yang1, Jing Fan1, Lian Jin1, Hongna Liu1, Yan Deng1,4, Song Li1,4, Xin Zeng1,2✉, Sauli Elingarami1, Nongyue He1✉

1. The State Key Laboratory of Bioelectronics, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;
2. Nanjing Maternity and Child Health Care Hospital, Nanjing 210029, China;
3. Nanjing Medical University, Nanjing 210029, China;
4. Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, P. R. China.

Citation:
Liu M, Hu P, Zhang G, Zeng Y, Yang H, Fan J, Jin L, Liu H, Deng Y, Li S, Zeng X, Elingarami S, He N. Copy Number Variation Analysis by Ligation-Dependent PCR Based on Magnetic Nanoparticles and Chemiluminescence. Theranostics 2015; 5(1):71-85. doi:10.7150/thno.10117. https://www.thno.org/v05p0071.htm
Other styles

File import instruction

Abstract

Graphic abstract

A novel system for copy number variation (CNV) analysis was developed in the present study using a combination of magnetic separation and chemiluminescence (CL) detection technique. The amino-modified probes were firstly immobilized onto carboxylated magnetic nanoparticles (MNPs) and then hybridized with biotin-dUTP products, followed by amplification with ligation-dependent polymerase chain reaction (PCR). After streptavidin-modified alkaline phosphatase (STV-AP) bonding and magnetic separation, the CL signals were then detected. Results showed that the quantification of PCR products could be reflected by CL signal values. Under optimum conditions, the CL system was characterized for quantitative analysis and the CL intensity exhibited a linear correlation with logarithm of the target concentration. To validate the methodology, copy numbers of six genes from the human genome were detected. To compare the detection accuracy, multiplex ligation-dependent probe amplification (MLPA) and MNPs-CL detection were performed. Overall, there were two discrepancies by MLPA analysis, while only one by MNPs-CL detection. This research demonstrated that the novel MNPs-CL system is a useful analytical tool which shows simple, sensitive, and specific characters which are suitable for CNV analysis. Moreover, this system should be improved further and its application in the genome variation detection of various diseases is currently under further investigation.

Keywords: Copy number variation, Ligation-dependent PCR, Magnetic nanoparticles, Chemiluminescence.


Citation styles

APA
Liu, M., Hu, P., Zhang, G., Zeng, Y., Yang, H., Fan, J., Jin, L., Liu, H., Deng, Y., Li, S., Zeng, X., Elingarami, S., He, N. (2015). Copy Number Variation Analysis by Ligation-Dependent PCR Based on Magnetic Nanoparticles and Chemiluminescence. Theranostics, 5(1), 71-85. https://doi.org/10.7150/thno.10117.

ACS
Liu, M.; Hu, P.; Zhang, G.; Zeng, Y.; Yang, H.; Fan, J.; Jin, L.; Liu, H.; Deng, Y.; Li, S.; Zeng, X.; Elingarami, S.; He, N. Copy Number Variation Analysis by Ligation-Dependent PCR Based on Magnetic Nanoparticles and Chemiluminescence. Theranostics 2015, 5 (1), 71-85. DOI: 10.7150/thno.10117.

NLM
Liu M, Hu P, Zhang G, Zeng Y, Yang H, Fan J, Jin L, Liu H, Deng Y, Li S, Zeng X, Elingarami S, He N. Copy Number Variation Analysis by Ligation-Dependent PCR Based on Magnetic Nanoparticles and Chemiluminescence. Theranostics 2015; 5(1):71-85. doi:10.7150/thno.10117. https://www.thno.org/v05p0071.htm

CSE
Liu M, Hu P, Zhang G, Zeng Y, Yang H, Fan J, Jin L, Liu H, Deng Y, Li S, Zeng X, Elingarami S, He N. 2015. Copy Number Variation Analysis by Ligation-Dependent PCR Based on Magnetic Nanoparticles and Chemiluminescence. Theranostics. 5(1):71-85.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
Popup Image