Theranostics 2016; 6(8):1119-1130. doi:10.7150/thno.13944 This issue Cite

Research Paper

Recapitulating the Size and Cargo of Tumor Exosomes in a Tissue-Engineered Model

Aranzazu Villasante1, Alessandro Marturano-Kruik1,4, Srikanth R. Ambati2, Zen Liu1, Amandine Godier-Furnemont1, Hesam Parsa1, Benjamin W. Lee1, Malcolm A.S. Moore3, Gordana Vunjak-Novakovic1✉

1. Department of Biomedical Engineering, Columbia University, New York, New York 10032, USA;
2. Department of Pediatrics, Memorial Sloan-Kettering Cancer Center, 1275 York Ave, New York, NY 10065, USA;
3. Department of Cell Biology, Memorial Sloan-Kettering Cancer Center, 1275 York Ave, New York, NY 10065, USA;
4. Department of Chemistry, Materials and Chemical Engineering "G Natta", Politecnico di Milano, Milano, Italy.

Citation:
Villasante A, Marturano-Kruik A, Ambati SR, Liu Z, Godier-Furnemont A, Parsa H, Lee BW, Moore MAS, Vunjak-Novakovic G. Recapitulating the Size and Cargo of Tumor Exosomes in a Tissue-Engineered Model. Theranostics 2016; 6(8):1119-1130. doi:10.7150/thno.13944. https://www.thno.org/v06p1119.htm
Other styles

File import instruction

Abstract

Graphic abstract

There is a growing interest in the pivotal role of exosomes in cancer and in their use as biomarkers. However, despite the importance of the microenvironment for cancer initiation and progression, monolayer cultures of tumor cells still represent the main in vitro source of exosomes. As a result, their environmental regulation remains largely unknown. Here, we report a three-dimensional tumor model for studying exosomes, using Ewing's sarcoma type 1 as a clinically relevant example. The bioengineered model was designed based on the hypothesis that the 3-dimensionality, composition and stiffness of the tumor matrix are the critical determinants of the size and cargo of exosomes released by the cancer cells. We analyzed the effects of the tumor microenvironment on exosomes, and the effects of exosomes on the non-cancer cells from the bone niche. Exosomes from the tissue-engineered tumor had similar size distribution as those in the patients' plasma, and were markedly smaller than those in monolayer cultures. Bioengineered tumors and the patients' plasma contained high levels of the Polycomb histone methyltransferase EZH2 mRNA relatively to their monolayer counterparts. Notably, EZH2 mRNA, a potential tumor biomarker detectable in blood plasma, could be transferred to the surrounding mesenchymal stem cells. This study provides the first evidence that an in vitro culture environment can recapitulate some properties of tumor exosomes.

Keywords: Exosomes, tumor models, microenvironment, tissue engineering, EZH2.


Citation styles

APA
Villasante, A., Marturano-Kruik, A., Ambati, S.R., Liu, Z., Godier-Furnemont, A., Parsa, H., Lee, B.W., Moore, M.A.S., Vunjak-Novakovic, G. (2016). Recapitulating the Size and Cargo of Tumor Exosomes in a Tissue-Engineered Model. Theranostics, 6(8), 1119-1130. https://doi.org/10.7150/thno.13944.

ACS
Villasante, A.; Marturano-Kruik, A.; Ambati, S.R.; Liu, Z.; Godier-Furnemont, A.; Parsa, H.; Lee, B.W.; Moore, M.A.S.; Vunjak-Novakovic, G. Recapitulating the Size and Cargo of Tumor Exosomes in a Tissue-Engineered Model. Theranostics 2016, 6 (8), 1119-1130. DOI: 10.7150/thno.13944.

NLM
Villasante A, Marturano-Kruik A, Ambati SR, Liu Z, Godier-Furnemont A, Parsa H, Lee BW, Moore MAS, Vunjak-Novakovic G. Recapitulating the Size and Cargo of Tumor Exosomes in a Tissue-Engineered Model. Theranostics 2016; 6(8):1119-1130. doi:10.7150/thno.13944. https://www.thno.org/v06p1119.htm

CSE
Villasante A, Marturano-Kruik A, Ambati SR, Liu Z, Godier-Furnemont A, Parsa H, Lee BW, Moore MAS, Vunjak-Novakovic G. 2016. Recapitulating the Size and Cargo of Tumor Exosomes in a Tissue-Engineered Model. Theranostics. 6(8):1119-1130.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
Popup Image