1. School of Life Science, South China Normal University, Guangzhou 510631, China. 2. MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China. * These authors contributed equally to this work and should be considered co-first author.
✉ Corresponding author: Chuhua Li, School of Life Science, South China Normal University, Guangzhou 510631, China. E-mail address: lichedu.cn (C. H. Li). Yan-Qing Guan, College of Biophotonics, South China Normal University, Guangzhou 510631, China. Tel.: (+86-20)85211241; E-mail address: guanyqedu.cn (Y. Q. Guan).More
Citation:
Niu S, Zhang LK, Zhang L, Zhuang S, Zhan X, Chen WY, Du S, Yin L, You R, Li CH, Guan YQ. Inhibition by Multifunctional Magnetic Nanoparticles Loaded with Alpha-Synuclein RNAi Plasmid in a Parkinson's Disease Model. Theranostics 2017; 7(2):344-356. doi:10.7150/thno.16562. https://www.thno.org/v07p0344.htm
Lewy bodies are considered as the main pathological characteristics of Parkinson's disease (PD). The major component of Lewy bodies is α-synuclein (α-syn). The use of gene therapy that targeting and effectively interfere with the expression of α-syn in neurons has received tremendous attention. In this study, we used magnetic Fe3O4 nanoparticles coated with oleic acid molecules as a nano-carrier. N-isopropylacrylamide derivative (NIPAm-AA) was photo-immobilized onto the oleic acid molecules, and shRNA (short hairpin RNA) was absorbed. The same method was used to absorb nerve growth factor (NGF) to NIPAm-AA to specifically promote neuronal uptake via NGF receptor-mediated endocytosis. Additionally, shRNA plasmid could be released into neurons because of the temperature and pH sensitivity of NIPAm-AA interference with α-syn synthesis. We investigated apoptosis in neurons with abrogated α-syn expression in vitro and in vivo. The results demonstrated that multifunctional superparamagnetic nanoparticles carrying shRNA for α-syn could provide effective repair in a PD model.
Niu, S., Zhang, L.K., Zhang, L., Zhuang, S., Zhan, X., Chen, W.Y., Du, S., Yin, L., You, R., Li, C.H., Guan, Y.Q. (2017). Inhibition by Multifunctional Magnetic Nanoparticles Loaded with Alpha-Synuclein RNAi Plasmid in a Parkinson's Disease Model. Theranostics, 7(2), 344-356. https://doi.org/10.7150/thno.16562.
ACS
Niu, S.; Zhang, L.K.; Zhang, L.; Zhuang, S.; Zhan, X.; Chen, W.Y.; Du, S.; Yin, L.; You, R.; Li, C.H.; Guan, Y.Q. Inhibition by Multifunctional Magnetic Nanoparticles Loaded with Alpha-Synuclein RNAi Plasmid in a Parkinson's Disease Model. Theranostics 2017, 7 (2), 344-356. DOI: 10.7150/thno.16562.
NLM
Niu S, Zhang LK, Zhang L, Zhuang S, Zhan X, Chen WY, Du S, Yin L, You R, Li CH, Guan YQ. Inhibition by Multifunctional Magnetic Nanoparticles Loaded with Alpha-Synuclein RNAi Plasmid in a Parkinson's Disease Model. Theranostics 2017; 7(2):344-356. doi:10.7150/thno.16562. https://www.thno.org/v07p0344.htm
CSE
Niu S, Zhang LK, Zhang L, Zhuang S, Zhan X, Chen WY, Du S, Yin L, You R, Li CH, Guan YQ. 2017. Inhibition by Multifunctional Magnetic Nanoparticles Loaded with Alpha-Synuclein RNAi Plasmid in a Parkinson's Disease Model. Theranostics. 7(2):344-356.