Theranostics 2018; 8(8):2289-2299. doi:10.7150/thno.23483 This issue Cite

Research Paper

Paper-based electrochemiluminescence sensor for highly sensitive detection of amyloid-β oligomerization: Toward potential diagnosis of Alzheimer's disease

Hongxing Liu, Xiaoming Zhou, Qi Shen, Da Xing

MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China

Citation:
Liu H, Zhou X, Shen Q, Xing D. Paper-based electrochemiluminescence sensor for highly sensitive detection of amyloid-β oligomerization: Toward potential diagnosis of Alzheimer's disease. Theranostics 2018; 8(8):2289-2299. doi:10.7150/thno.23483. https://www.thno.org/v08p2289.htm
Other styles

File import instruction

Abstract

Graphic abstract

Development of a rapid and sensitive method for Aβ(1-42) aggregation detection is of great importance to overcome the limitations of conventional techniques. In this study, we developed a label-free paper-based electrochemiluminescence sensor for amyloid-β aggregation detection toward potential diagnosis of Alzheimer's disease (AD). The paper-based chip used in the system serves as a low-cost and disposable detection method. In this detection platform, the bonding of [Ru(phen)2dppz]2+ to Aβ(1-42) aggregates results in enhanced electrochemiluminescence due to the change in the polarity of the microenvironment when [Ru(phen)2dppz]2+ intercalated into the β-sheets during oligomerization. The oligomerization process of Aβ(1-42) can be monitored in real time by the novel method, and as low as 100 pM equivalent monomer concentration of Aβ(1-42) could be detected simultaneously. In addition, the cerebrospinal fluid of transgenic AD model mice was tested by this method, which is highly consistent with genetic identification. In addition, we demonstrated that this detection platform could be a potential new method for the screening of Aβ(1-42) aggregation inhibitors, highlighting the practical application capacity of this platform. The platform is label free, low cost and sensitive. Therefore, the proposed platform holds great promise for the diagnosis of AD.

Keywords: Aβ(1-42) aggregation, electrochemiluminescence, [Ru(phen)2dppz]2+, paper-based bipolar electrode, Alzheimer's disease


Citation styles

APA
Liu, H., Zhou, X., Shen, Q., Xing, D. (2018). Paper-based electrochemiluminescence sensor for highly sensitive detection of amyloid-β oligomerization: Toward potential diagnosis of Alzheimer's disease. Theranostics, 8(8), 2289-2299. https://doi.org/10.7150/thno.23483.

ACS
Liu, H.; Zhou, X.; Shen, Q.; Xing, D. Paper-based electrochemiluminescence sensor for highly sensitive detection of amyloid-β oligomerization: Toward potential diagnosis of Alzheimer's disease. Theranostics 2018, 8 (8), 2289-2299. DOI: 10.7150/thno.23483.

NLM
Liu H, Zhou X, Shen Q, Xing D. Paper-based electrochemiluminescence sensor for highly sensitive detection of amyloid-β oligomerization: Toward potential diagnosis of Alzheimer's disease. Theranostics 2018; 8(8):2289-2299. doi:10.7150/thno.23483. https://www.thno.org/v08p2289.htm

CSE
Liu H, Zhou X, Shen Q, Xing D. 2018. Paper-based electrochemiluminescence sensor for highly sensitive detection of amyloid-β oligomerization: Toward potential diagnosis of Alzheimer's disease. Theranostics. 8(8):2289-2299.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Popup Image