Combined BET and MEK Inhibition synergistically suppresses melanoma by targeting YAP1

Rationale: The response rate to the MEK inhibitor trametinib in BRAF-mutated melanoma patients is less than 30%, and drug resistance develops rapidly, but the mechanism is still unclear. Yes1-associated transcriptional regulator (YAP1) is highly expressed in melanoma and may be related to MEK inhibitor resistance. The purpose of this study was to investigate the mechanism of YAP1 in MEK inhibitor resistance in melanoma and to screen YAP1 inhibitors to further determine whether YAP1 inhibition reverses MEK inhibitor resistance. Methods: On the one hand, we analyzed paired melanoma and adjacent tissue samples using RNA-seq and found that the Hippo-YAP1 signaling pathway was the top upregulated pathway. On the other hand, we evaluated the transcriptomes of melanoma samples from patients before and after trametinib treatment and investigated the correlation between YAP1 expression and trametinib resistance. Then, we screened for inhibitors that repress YAP1 expression and investigated the mechanisms. Finally, we investigated the antitumor effect of YAP1 inhibition combined with MEK inhibition both in vitro and in vivo. Results: We found that YAP1 expression levels upon trametinib treatment in melanoma patients were correlated with resistance to trametinib. YAP1 was translocated into the nucleus after trametinib treatment in melanoma cells, which could render resistance to MEK inhibition. Thus, we screened for inhibitors that repress YAP1 expression and identified multiple bromodomain and extra-terminal (BET) inhibitors, including NHWD-870, as hits. BET inhibition repressed YAP1 expression by decreasing BRD4 binding to the YAP1 promoter. Consistently, YAP1 overexpression was sufficient to reverse the proliferation defect caused by BRD4 depletion. In addition, the BET inhibitor NHWD-870 acted synergistically with trametinib to suppress melanoma growth in vitro and in vivo. Conclusions: We identified a new vulnerability for MEK inhibitor-resistant melanomas, which activated Hippo pathway due to elevated YAP1 activity. Inhibition of BRD4 using BET inhibitors suppressed YAP1 expression and led to blunted melanoma growth when combined with treatment with the MEK inhibitor trametinib.


Introduction
Melanoma, the deadliest form of skin cancer, has a rising incidence and a five-year survival of only 27% for melanoma patients with distant metastasis [1].Immune checkpoint blockade, including anti-PD-1 and anti-CTLA-4 therapy, has brought effective and sustainable benefits to late-stage melanoma patients, with a response rate of approximately 30% [2], and MAPK inhibition therapy was reported to be effective in approximately 50% of melanomas with a BRAF mutation [3].Trametinib is an FDA-approved MEK inhibitor that benefits only a subset of melanoma patients [4], but the mechanisms of resistance are still unclear.Therefore, new therapeutics for use alone or in combination with MEK inhibitors are urgently needed to inhibit melanoma progression.
YAP1 (Yes1-associated transcriptional regulator) is a transcriptional coactivator that fuels several hallmarks of cancer [5], including tumor initiation, cell plasticity, drug resistance and metastasis [6].In melanoma patient samples, YAP1 is reported to be highly expressed due to copy number alteration, and its expression is correlated with poor prognosis [7].Several studies have revealed that YAP1 plays a critical role in melanoma proliferation and metastasis [8,9], suggesting that YAP1 is a potential therapeutic target.Furthermore, high YAP1 levels have been shown to be correlated with resistance to trametinib in neuroblastomas [10].YAP1 depletion sensitizes neuroblastoma to trametinib, and overexpression of YAP1 induces trametinib resistance in neuroblastoma cells [10].
Emerging evidence has shown that epigenetic aberrations contribute to drug resistance.Among the epigenetic readers, the bromodomain and extra-terminal (BET) family proteins, which includes Bromodomain Containing 2 (BRD2), Bromodomain Containing 3 (BRD3), Bromodomain Containing 4 (BRD4) and Bromodomain testis-specific protein (BRDT), have been shown to be promising therapeutic targets in multiple types of cancer [11,12].BRD2, BRD3 and BRD4 have been found to be expressed in a spectrum of tissues, while the expression of BRDT is limited to the testes [13].The two bromodomains recognize and bind to acetylated histone tails and promote transcriptional activation and elongation, which support uncontrolled proliferation by tumor cells [14].Recent studies have revealed that the functional target of BET proteins varies in different types of cancer [15][16][17]; for example, p21 is the target in NSCLC [18], and AR is the target in prostate cancer [19].We previously reported the development of a novel and potent BET inhibitor, NHWD-870, aiming to improve the efficacy of BET inhibition in solid tumors, and NHWD-870 showed high potency and tolerable toxicity in vivo [20].NHWD-870 has entered a phase I clinical trial for multiple cancer indications in China (CXHL200250).
Here, we found that YAP1 expression was significantly increased after trametinib treatment in melanoma patients who did not respond to trametinib and correlated with poor survival of melanoma patients treated with trametinib.We also found that a MEK inhibitor mediated the translocation of YAP1 in melanoma cells, suggesting that YAP1 inhibition may sensitize melanoma to trametinib.Although there is no inhibitor that acts directly on YAP1 in use in the clinic because of the unstructured nature of YAP1 [21], the combinatorial use of indirect inhibitors of YAP1 and MEK inhibitor may serve as an alternative avenue for melanoma treatment.In an effort to find YAP1 inhibitors, we screened 102 small molecules that were FDA-approved, in clinical trials, or druglike, and found that the BET inhibitor NHWD-870 strongly suppressed YAP1 expression and melanoma proliferation.Furthermore, the combination of a BET inhibitor and a MEK inhibitor synergistically suppressed melanoma progression, providing a promising therapeutic option for melanoma patients.

Nuclear YAP1 expression is positively correlated with resistance to the MEK inhibitor trametinib and poor prognosis of melanoma patients
We analyzed paired melanoma and adjacent tissue samples from 23 patients 18 acral melanomas, 2 sun-exposed melanomas and 3 unclear-subset melanomas using RNA-seq and found that the Hippo-YAP1 signaling pathway was a top upregulated pathway in melanoma compared with paired adjacent tissues (Figure 1A).Furthermore, TCGA database analysis results showed that high expression of YAP1 seems to have poorer prognosis of skin cutaneous melanoma (SKCM) although P values showed no difference between high and low YAP1 expression (Figure 1B).Thus, we compared YAP1 expression before and after trametinib treatment.While there was no significant difference in nuclear YAP1 expression in responders before versus after treatment, trametinib treatment significantly increased nuclear YAP1 expression in nonresponders (Figure 1C-D).In addition, there was no significant difference in nuclear YAP1 expression between responders and nonresponders before trametinib treatment (Figure 1C-D), but nuclear YAP1 expression was significantly increased in nonresponders after trametinib treatment (Figure 1C).Moreover, the change in nuclear YAP1 expression after trametinib treatment was significantly greater in nonresponders than in responders (Figure 1E).Then, we correlated nuclear YAP1 expression with clinical outcome and found that the change in nuclear YAP1 expression was higher in patients with poor clinical outcomes than in those with good clinical outcomes before trametinib treatment (Figure S1A).Moreover, the change in nuclear YAP1 expression after trametinib treatment was significantly greater in patients with poor clinical outcomes than in patients with good clinical outcomes (Figure S1B).Consistently, Kaplan-Meier analysis showed that the change in nuclear YAP1 expression after trametinib treatment was significantly correlated with poor prognosis in these melanoma patients (Figure 1F).To further investigate the role of YAP1 in melanoma progression, we analyzed YAP1 expression in the tumor stroma of patients who responded and nonresponded to trametinib treatment.The results showed that there was no difference in the expression of YAP1 in the stroma between nonresponders and responders before and after treatment (Figure S1C), and it was not related to the prognosis of patients (Figure S1D-F).These results prompted us to investigate whether YAP1 nuclear localization in melanoma cells changes after trametinib treatment.Immunofluorescence analysis showed that YAP1 was translocated into the nucleus after trametinib treatment in A375 and SK-MEL-28 melanoma cells (Figure 1G-H).Our findings suggested that YAP1 upregulation could account for the low sensitivity of MEK inhibition, consistent with the findings of a previous study [22].Thus, suppression of YAP1 might improve the therapeutic effect of trametinib in melanoma patients.

YAP1 is necessary for melanoma growth
The expression of YAP1 is correlated with stemness score, TOP2A and KI67 (Figure S2A), suggesting that YAP1 may be related to cell proliferation.Therefore, we used the CRISPR-Cas9 system and siRNAs, respectively, to delete YAP1 in A375 cells and SK-MEL-28 cells (Figure 2A) and performed short-term proliferation assays as well as long-term colony formation assays (Figure 2B-C).Depletion of YAP1 in the A375 and SK-MEL-28 cell lines significantly inhibited the proliferation of melanoma cells.To further investigate the roles of YAP1, we injected A375 and YAP1 knockout cells into nude mice and measured their ability to form tumors.We found that YAP1 knockout blunted tumor growth and prolonged the survival of tumor-bearing mice (Figure 2D).Next, we established YAP1 overexpression in A375 and SK-MEL-28 (Figure 2E) cell lines for further validation.The results demonstrated that the overexpression of YAP1 in A375 and SK-MEL-28 significantly enhanced the proliferation of melanoma cells (Figure 2F-G).Moreover, the animal experiments corroborated these findings by revealing that YAP1 overexpression not only promoted tumor growth but also significantly reduced the survival rate of tumor-bearing mice (Figure 2H).These data suggest that YAP1 is necessary for melanoma growth in vitro and in vivo.

Multiple BET inhibitors are inhibitors of YAP1 expression
To target YAP1, we screened 102 small molecules that are FDA approved, currently in clinical trials, or druglike inhibitors of YAP1 expression in A375 cells.We found that multiple BET inhibitors, including NHWD-870, BMS-986158, OTX-015 and JQ1, were top hits that decreased YAP1 expression (Figure 3A-B).To further investigate the roles of BET inhibitors in melanoma, we performed RT-qPCR and western blot analyses for YAP1 expression in A375 and SK-MEL-28 cells treated with different doses of NHWD-870 and JQ1.Both inhibitors decreased the mRNA and protein levels of YAP1 in a dose-dependent manner, and NHWD-870 showed a significantly stronger inhibitory effect than JQ1 at the same dose (Figure 3C-E).We also treated a panel of melanoma cells, including YUSOC, YUGASP, YUAME, YUMAC and SK-MEL-28 cells, with 10 nM NHWD-870 and found that YAP1 protein levels decreased in all these different melanoma cells (Figure 3F).NHWD-870 led to decreased proliferation and colony-forming ability of A375 and SK-MEL-28 cells in a dose-dependent manner in vitro (Figure 3G-I).We then treated A375 tumor-bearing mice with NHWD-870 and found that tumor growth was significantly attenuated in vivo (Figure 3J).Histological analysis revealed that Ki67 levels were decreased in tumors treated with NHWD-870 (Figure 3K).In addition, IHC analysis showed that YAP1 expression was decreased in tumors treated with NHWD-870 (Figure 3L).Together, these data suggest that BET inhibitors suppress melanoma proliferation by downregulating YAP1 expression.

YAP1 is a direct downstream effector of BRD4 mediating melanoma proliferation
The elevated expression of BRD2/BRD3/ BRD4/YAP1 is a risk factor from the Cox cross-pan-cancer dataset proportional hazards model in a variety of tumors, including SKCM (Figure 4A), besides, there was an association between BRD4 and YAP1 (Figure 4B).To further verify the possible mechanisms through which BET inhibitors suppress YAP1 expression, we knocked down BRD2, BRD3 and BRD4 in A375 and SK-MEL-28 cells with siRNAs.We found that only BRD4 knockdown decreased the expression of YAP1 (Figure S3A-B and Figure 4C).Using the CRISPR-Cas9 system, we found that deletion of BRD4 in A375 and SK-MEL-28 cells recapitulated the effects of BET inhibitors (Figure 4D-E).Cell proliferation assays and clonogenic assays also showed that BRD4 knockout decreased melanoma proliferation (Figure 4F-G).Consistently, BRD4 deletion slowed the growth of A375 tumors in nude mice (Figure 4H).Histological analysis revealed that Ki67 and YAP1 expression was decreased in BRD4-knockout tumors (Figure 4I-J).These data suggest that BRD4 strongly decreases YAP1 expression and melanoma growth.To determine whether BRD4 directly regulates YAP1, we performed ChIP-seq analysis and found a BRD4-binding peak on the YAP1 promoter in A375 cells.This peak was decreased in A375 cells treated with NHWD-870 (Figure 4K top panel).These data were consistent with the Zhang laboratory's ChIP-seq data, which showed that BRD4 binding to the YAP1 promoter was attenuated by JQ1 treatment and enhanced in BRD4-overexpressing cells (Figure 4K bottom panel) [23].These results indicate that BRD4 promotes melanoma progression through direct regulation of YAP1 (Figure 4L).Furthermore, we overexpressed YAP1 in the BRD4-knockout A375 and SK-MEL-28 cell lines (Figure 5A-B) and performed proliferation assays and xenograft studies.The overexpression of YAP1 completely reversed the anti-proliferation phenotype of BRD4 knockout both in vitro and in vivo (Figure 5C-H).Together, these data indicate that the BRD4-YAP1 axis is critical for melanoma proliferation.

Combined BET and MEK inhibition synergistically suppresses melanoma growth
We next investigated the efficacy of the combination of BET and MEK inhibition.We treated melanoma cell line A375, SK-MEL-28 and colorectal cancer cell line HT29 with NHWD-870 and trametinib in vitro, and flow cytometry analysis revealed that Ki67 expression was strongly decreased in the combination group (Figure 6A).Moreover, we found that the combination synergistically inhibited melanoma cell growth as measured by CCK-8 cell proliferation assays (Figure 6B-D).Combination index (CI) analysis and normalized isobolograms for trametinib and NHWD-870 combination further supported the synergistic nature of this combination (Figure 6E and Figure S4).To test its efficacy in vivo, we treated A375 tumor-bearing nude mice with NHWD-870, trametinib or their combination.Consistent with our in vitro findings, NHWD-870 and trametinib acted in synergy in vivo (Figure 6F).

BRD4 expression is correlated with YAP1 expression, lack of response, and poor survival of melanoma patients treated with the MEK inhibitor trametinib
We analyzed BRD4 expression in patients with melanoma before or after trametinib treatment.Consistent with the change in YAP1 expression, there was no significant difference in BRD4 expression in responders before versus after treatment.However, trametinib treatment significantly increased BRD4 expression in nonresponders but not in responders (Figure 7A-B).Our results also showed that BRD4 expression in nonresponders was higher than that in responders before treatment (Figure 7B).In addition, the increase in BRD4 expression (BRD4post-BRD4prior) was higher in nonresponders after trametinib treatment than in responders (Figure 7C).Consistently, BRD4 expression in patients with poor clinical outcomes was higher than that in patients with good clinical outcomes before trametinib treatment (Figure S5A).In addition, the change in BRD4 expression was significantly elevated after trametinib treatment in the poor-clinical-outcome group compared with the good-clinical-outcome group (Figure S5B).Furthermore, the change in BRD4 expression (BRD4post-BRD4prior) was positively correlated with the poor prognosis of patients with melanoma treated with trametinib (Figure 7D).The change in BRD4 expression was also positively correlated with the change in YAP1 expression (p = 0.0017, R 2 = 0.5152) (Figure 7E).These results indicate that both YAP1 expression and BRD4 expression are correlated with the response and the outcome of trametinib treatment in patients with melanoma.

Cell culture
A375 and SK-MEL-28 cells were obtained from the ATCC (Manassas, USA).Both were cultured in DMEM supplemented with 10% FBS, 100 U/mL penicillin and 100 mg/mL streptomycin.YUSOC, YUGASP, YUAME, and YUMAC are cell lines derived from tumors of patients treated at Yale University and were grown in Opti-MEM plus with 5% FBS.

Clinical samples
All clinical specimens in this study were collected with informed consent for research use and were approved by Central South University Institutional Review Boards in accordance with the Declaration of Helsinki.Melanoma tumor specimens were excised to alleviate tumor burden.The data referenced in this study are available under GSE190113 in the Gene Expression Omnibus (GEO).After institutional review board approval, we collected melanoma tissues from patients who met our inclusion criteria from January 2017 to December 2020 in the Tumor Hospital of Harbin Medical University.All the patients enrolled for the study were ≥18 years and had histologically confirmed unresectable stage IIIC or IV melanoma with a mutation at the 600th position in BRAF."Good outcome" and "Poor outcome" means the patients were still alive or dead when we collected the date in May, 2021.

Immunofluorescence study
Immunofluorescence staining of melanoma tumor sections was performed according to the manufacturer's instructions.Quantitative comparison of YAP1 (ab56701, Abcam,1:400), and BRD4 (#13440, CST 1:200) expression was performed using freeware image analysis software (ImageJ, WS Rasband, National Health Institute, Bethesda, MA, USA) as previously reported [24].The cell area was determined by manual delineation of raw fluorescence images.A minimum of 12 cells were analyzed from two independent experiments.

RT-qPCR
Total RNA was isolated with TRIzol reagent (15596026, Invitrogen) according to the manufacturer's instructions.cDNA was synthesized with HiScript® II Q RT SuperMix for qPCR (+gDNA Wiper) following the manufacturer's instructions (R223-01, Vazyme).RT-qPCR was performed with 2X SYBR Green qPCR Master Mix (B21703, Bimake) using an ABI QuantStudio 3 PCR system.The expression of RNA is shown relative to the level of GAPDH mRNA.The primers used for RT-qPCR were as follows:

ChIP-seq
Chromatin immunoprecipitation (ChIP)-seq was performed by Acegen.Briefly, A375 cells were treated with 4 nM NHWD-870 for 3 days.Then, those cells were collected and cross-linked in DMEM with 1% formaldehyde.A Bioruptor was used to sonicate chromatin, after which incubation was performed with a BRD4 antibody (#13440, CST) for immunoprecipitation.The DNA library was prepared using an Acegen DNA Library Prep Kit from Illumina, amplified by twelve-cycle PCR, cleaned up, analyzed with an Agilent 2100 Bioanalyzer and finally sequenced on the Illumina platform.

Colony formation assay and cell proliferation assay
For the colony formation assay, A375 cells or A375-derived cells were seeded into 6-well plates at 1000 cells per well.Seven days later, 10% formalin was used to fix the cells for 20 minutes at room temperature.Then, they were stained with 0.05% crystal violet in distilled water for 1 hour.Afterward, they were washed with water 3 times.Pictures were captured using a scanner.A CCK-8 assay was used to assess cell proliferation.A375 cells or SK-MEL-28 cells were seeded into 96-well plates at 800 cells per well and incubated for 1-5 days.At various time points, the medium was replaced with fresh medium containing 10% CCK-8 reagent (Bimake, Cat# B34302, USA).After incubation for 2.5 h, the absorbance at 450 nm was measured.For combinational treatments, the combination index (CI) was calculated using CompuSyn software (ComboSyn Inc., Biosoft; Cambridge, UK).

Ki67-positive cell analysis
A375 cells were seeded into 6-well plates at 5 × 10 5 cells per well.After 12 h, the cells were treated with NHWD-870 and/or trametinib.After incubation for 3 days, an anti-Ki67-FITC antibody (Thermo Fisher Scientific, #11-5698-80) was used to stain the cells.Ki67-positive cells were detected by flow cytometry and analyzed using FlowJo software.

Animal studies
A total of 1x10 6 A375-or A375-derived cells were subcutaneously implanted into the flanks of female nude mice.For drug treatment, when the tumors reached approximately 100 mm 3 , 0.5 mg/kg NHWD-870 once daily (5 days on, 2 days off) and/or 0.5 mg/kg trametinib once daily or vehicle was administered to the mice orally [20,25,26].The tumor sizes were measured every 3 or 4 days using a caliper.All mice were sacrificed by cervical dislocation after intraperitoneal injection with 0.1% pentobarbital sodium (30 mg/kg), and the tumors were dissected and fixed in formalin.All animals were housed in the specific pathogen-free animal facilities of Central South University with a 12:12 h light-dark cycle at a constant room temperature (22 ±1 °C) and fed a standard laboratory diet.

Conclusion
In summary, we identified a new vulnerability for MEK inhibitor-resistant melanomas, which have an activated Hippo pathway due to elevated YAP1 activity.Inhibition of BRD4 using BET inhibitors suppressed YAP1 expression and led to blunted melanoma growth when combined with treatment with the MEK inhibitor trametinib.Thus, these results suggest a promising combination therapy strategy for melanoma treatment.

Ethics approval and consent to participate
The study was approved by the Medical Ethics Committee of Xiangya Hospital of Central South University (permit number: 202004337).The animal study was approved by Animal Ethics Committee of Central South University Institutional Review Boards (permit number: 2020sydw0679).

Data collection
The gene expression profile and the corresponding clinical data of 33 cancer types from The Cancer Genome Atlas (TCGA) research network were collected using TCGAbiolinks R package.In our study, we only considered primary tumor samples and using TCGA barcode (https://docs.gdc.cancer.gov/Encyclopedia/pages/TCGA_Barcode/) to retain them.

Exploring the association between YAP1 and tumor proliferation in cutaneous melanoma
Signature of stemness was downloaded from MSigDB website (http://www.gsea-msigdb.org/gsea/msigdb/cards/MALTA_CURATED_STEMNES S_MARKERS).We calculated stemness score across cutaneous melanoma (TCGA-SKCM) samples using GSVA R package.After that, the association between YAP1 expression and stemness score were determined using Spearman correlation test.Besides, we also tested the association between YAP1 and two proliferation related genes (MKI67 and TOP2A) based on Spearman correlation test.

Assessment of the correlation between YAP1 and BRD4 protein across pan-cancer datasets
The correlation between YAP1 and BRD4 protein was obtained using Spearman correlation test across 33 cancer types.Specifically, the correlation coefficient (R) and P value were used for downstream visualization.

Survival analysis
The association between the overall survival time of patients and the expression of investigated genes (YAP1, BRD2, BRD3, BRD4) were explored based on Cox proportional-hazards model.Genes with hazard ratio (HR) greater than 1 were regarded as risk factors, on the contrary, those with HR less than 1 were considered as protective factors.Kaplan-Meier survival curve was plotted between sub-groups that divided by the median of gene expression value.The Cox proportional-hazards model and Kaplan-Meier survival curve were implemented in survival R package.

Statistical analysis
All data from the experiments are expressed as the mean ± SEM.The data were statistically analyzed with an unpaired Student's t test or one-way ANOVA/Tukey's multiple comparisons test using GraphPad Prism 5 (GraphPad Software, La Jolla, CA).Overall survival (OS) was analyzed using the Kaplan-Meier method.Statistical significance was set at *P < 0.05, **P < 0.01, and ***P < 0.001.

Discussion
In this study, we first identified the Hippo-YAP signaling pathway as a top elevated pathway in patients with melanoma.Analysis of YAP1 expression in melanoma patients treated with trametinib showed that YAP1 expression was negatively correlated with the response and survival in these patients.Consistently, YAP1 was enriched in the nucleus after trametinib treatment in melanoma cells, suggesting that increased nuclear YAP1 could lead to resistance to MEK inhibition (Figure 7F).Therefore, we screened for inhibitors of YAP1 expression and identified multiple BET inhibitors as the top hits.Mechanistically, BRD4 binds the YAP1 promoter to activate its expression, and this regulation is blocked by BETi (Figure 7F).YAP1 overexpression is sufficient to rescue the proliferation defect caused by BRD4 depletion, suggesting that YAP1 is a critical downstream target of BRD4.Furthermore, the BET inhibitor NHWD-870 acts synergistically with the MEK inhibitor trametinib to suppress melanoma growth, suggesting a promising combination-therapy strategy for melanoma treatment.
The oncogenic role of BRD4 was first discussed when BRD4-NUT fusion was shown to initiate the development of NUT-midline carcinoma [27].The BRD4-NUT fusion activates prosurvival genes such as MYC to facilitate the proliferation of NUT-midline carcinoma [28].Preclinical and clinical studies have demonstrated the efficacy of BET inhibition in several hematological malignancies [29,30], in which inhibition of BRD4 leads to downregulation of MYC expression [31], a master regulator of cell survival and proliferation [32].However, the functional targets of BRD4 vary in other types of cancer.For example, the BET inhibitor JQ1 sensitized tumor cells to radiotherapy by regulating p21 in NSCLC [18].In prostate cancer, the BET inhibitor PFI-1 inhibits prostate cancer cell growth by suppressing the transactivation of full-length AR [19].In melanoma, a previous study failed to rescue the growth-inhibitory phenotype of BRD4 deletion with MYC overexpression [33], suggesting that MYC is not the main target of BRD4 in melanoma.Here, we demonstrated a strong correlation between BRD4 and YAP1 in melanoma patient samples.BRD4 bound to the YAP1 promoter, and overexpression of YAP1 successfully rescued the proliferation suppression caused by BRD4 knockout.Together, these results suggest that YAP1 is a main downstream effector of BRD4 in melanoma.
BET inhibition has emerged as an appealing avenue for cancer treatment [34].Several clinical trials have shown promising results in some hematological malignancies, but the efficacy in solid tumors is limited due to low potency and intrinsic resistance [35].Efforts have been made to address this limitation by combining BETi with other therapies.One study set out to find an effective combination therapy with BET inhibition by screening approximately 1900 small molecular inhibitors.That study revealed that PI3K inhibitors are the most potent partners against neuroblastoma in vitro and in vivo [36].Another study also demonstrated that this combination can induce cell death and tumor regression in some breast cancer models, providing a promising strategy for fighting kinase inhibitor therapy resistance [37].Moreover, in an ovarian cancer model, combination treatment with PI3K inhibitors and BET inhibitors was shown to suppress the proliferation of cancer cells resistant to MEK inhibition [38].Other interesting possibilities for combination treatment are HDAC inhibitors, which can elevate the global level of histone acetylation, facilitating BET protein binding to chromatin.The synergistic effects have been demonstrated in a MYC-driven lymphoma model and a neuroblastoma model [39,40].Here, we found that resistance to MEK inhibitors may be due to YAP1 upregulation, which can be overcome by BETi-mediated suppression of YAP1 expression.Furthermore, the combination of BETi and MEKi acts synergistically to suppress melanoma growth.Our results are consistent with those of previous studies on BETi and MEKi combinations in esophageal cancer and triple-negative breast cancer [41,42].Together, these results suggest that the combination of BETi and MEKi is a promising therapeutic strategy against multiple solid tumors.project administration, resources.

Figure 1 .
Figure 1.YAP1 expression is correlated with poor response to trametinib and survival of patients with melanoma.(A) Paired primary tumors and adjacent tissues from 23 melanoma patients were compared by using RNA-sequencing (RNA-seq) analysis.Shown are the pathways that were upregulated in the tumors.(B) Kaplan-Meier survival curve of two sub-groups that divided by YAP1 expression in TCGA-SKCM dataset.(C) YAP1 immunofluorescence (IF) staining of tumor tissues from responders and nonresponders before and after trametinib treatment.YAP1 (green) and DAPI (blue).(D) Fluorescence intensity of nuclear YAP1 expression in tumor tissue from responders and

Figure 7 .
Figure 7. BRD4 expression is correlated with YAP1 expression and poor response and survival of patients with melanoma treated with the MEK inhibitor trametinib.(A) Representative immunofluorescence staining of tumor tissue from responders and nonresponders before and after trametinib treatment.BRD4, green; DAPI, blue.(B) BRD4 expression in tumor tissue from responders and nonresponders before and after trametinib treatment.(C) Change in BRD4 expression (BRD4post-BRD4prior) in tumor tissue from responders and nonresponders before and after trametinib treatment.(D) Kaplan-Meier analysis of overall survival.The BRD4post-BRD4prior high group had a worse prognosis in terms of survival than the BRD4post-BRD4prior low group.(E) The change in BRD4 expression (BRD4post-BRD4prior) is positively correlated with the change in YAP1 expression (YAP1post-YAP1prior). (F) Working model summarizing the major findings.* p < 0.05, ** p < 0.01, *** p < 0.001.The error bars represent the SEMs.