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Theranostics 2016; 6(1):14-27. doi:10.7150/thno.13515 This issue Cite

Research Paper

Triple-Layered pH-Responsive Micelleplexes Loaded with siRNA and Cisplatin Prodrug for NF-Kappa B Targeted Treatment of Metastatic Breast Cancer

Haijun Yu1,#, ✉, Chengyue Guo1,2,#, Bing Feng1, Jianping Liu1, Xianzhi Chen1, Dangge Wang1, Lesheng Teng2, Youxin Li2, Qi Yin1, Zhiwen Zhang1, Yaping Li1,✉

1. State key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
2. College of Life Science, Jilin University, Changchun, 130012, China.
#: These two authors contributed equally to this study.

✉ Corresponding authors: Dr. Haijun Yu, Prof. Dr. Yaping Li, State key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. E-mail: hjyuac.cn, ypliac.cn; Tel/Fax: +86-21-2023-1979 More

Citation:
Yu H, Guo C, Feng B, Liu J, Chen X, Wang D, Teng L, Li Y, Yin Q, Zhang Z, Li Y. Triple-Layered pH-Responsive Micelleplexes Loaded with siRNA and Cisplatin Prodrug for NF-Kappa B Targeted Treatment of Metastatic Breast Cancer. Theranostics 2016; 6(1):14-27. doi:10.7150/thno.13515. https://www.thno.org/v06p0014.htm
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Abstract

Graphic abstract

The combination of chemotherapy and RNA interference is a promising approach for efficient cancer therapy. However, the success of such a strategy is hampered by the lack of suitable vectors to coordinate small interfering RNA (siRNA) and chemotherapeutic drug into one single platform. We herein report a novel triple-layered pH-responsive micelleplex loading siRNA and alkylated cisplatin prodrug for NF-Kappa B targeted treatment of metastatic breast cancer. The micelles were self-assembled from poly(ethylene glycol)-block-poly(aminolated glycidyl methacrylate)-block-poly(2-(diisopropyl amino) ethyl methacrylate) (PEG-b-PAGA-b-PDPA) triblock copolymers. At pH 7.4, the cisplatin prodrug was encapsulated in the hydrophobic PDPA core and siRNA was loaded on the positively charged PAGA interlayer to form the micelleplexes. The PEG corona can prevent protein absorption during blood circulation, minimize non-specific interaction with the reticuloendothelial system, and prolong the systemic circulation of the micelleplexes. The positively charged PAGA interlayer can facilitate deep tumor penetration of the micelleplexes, which, upon cellular uptake, are dissociated in the early endosomes to release anticancer drug payload due to protonation of the PDPA core. Using a 4T1 breast cancer model, we demonstrate that this novel micelleplex co-loaded with cisplatin prodrug and siRNA-p65 is able to simultaneously inhibit tumor growth and suppress distant metastasis of the cancer cells by downregulating NF-kappa B expression. The results reported in this study suggest that siRNA and anticancer drug co-delivery using pH-responsive micelleplexes is a promising strategy for efficient treatment of metastatic cancer.

Keywords: Cancer Metastasis, Micelleplexes, pH-Responsive, RNAi, Chemotherapy.

Citation styles

APA
Yu, H., Guo, C., Feng, B., Liu, J., Chen, X., Wang, D., Teng, L., Li, Y., Yin, Q., Zhang, Z., Li, Y. (2016). Triple-Layered pH-Responsive Micelleplexes Loaded with siRNA and Cisplatin Prodrug for NF-Kappa B Targeted Treatment of Metastatic Breast Cancer. Theranostics, 6(1), 14-27. https://doi.org/10.7150/thno.13515.

ACS
Yu, H.; Guo, C.; Feng, B.; Liu, J.; Chen, X.; Wang, D.; Teng, L.; Li, Y.; Yin, Q.; Zhang, Z.; Li, Y. Triple-Layered pH-Responsive Micelleplexes Loaded with siRNA and Cisplatin Prodrug for NF-Kappa B Targeted Treatment of Metastatic Breast Cancer. Theranostics 2016, 6 (1), 14-27. DOI: 10.7150/thno.13515.

NLM
Yu H, Guo C, Feng B, Liu J, Chen X, Wang D, Teng L, Li Y, Yin Q, Zhang Z, Li Y. Triple-Layered pH-Responsive Micelleplexes Loaded with siRNA and Cisplatin Prodrug for NF-Kappa B Targeted Treatment of Metastatic Breast Cancer. Theranostics 2016; 6(1):14-27. doi:10.7150/thno.13515. https://www.thno.org/v06p0014.htm

CSE
Yu H, Guo C, Feng B, Liu J, Chen X, Wang D, Teng L, Li Y, Yin Q, Zhang Z, Li Y. 2016. Triple-Layered pH-Responsive Micelleplexes Loaded with siRNA and Cisplatin Prodrug for NF-Kappa B Targeted Treatment of Metastatic Breast Cancer. Theranostics. 6(1):14-27.

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