Theranostics 2018; 8(4):941-954. doi:10.7150/thno.21173

Research Paper

Investigating the Intracellular Behaviors of Liposomal Nanohybrids via SERS: Insights into the Influence of Metal Nanoparticles

Dan Zhu, Zhuyuan Wang, Shenfei Zong, Yizhi Zhang, Chen Chen, Ruohu Zhang, Binfeng Yun, Yiping Cui

Advanced Photonics Center, Southeast University, 2# Sipailou, Nanjing 210096, Jiangsu, China

Abstract

The recent proposition to combine liposomes with nanoparticles presents great opportunities to develop multifunctional drug delivery platforms. Although impressive progress has been made, attempts to elucidate the role nanoparticles play in the integral nanohybrids are still rather limited. Here, using surface-enhanced Raman scattering (SERS) technique, we investigate the influence of metal nanoparticles on the liposomal properties, ranging from drug release to intracellular movement. Specifically, we prepared SERS-active nanohybrids by attaching metal nanoparticles to liposomes and employed SERS signals to explore the intracellular behavior of the nanohybrids. Once deposited on the cell membrane, the nanohybrids entered tumor cells via clathrin-mediated endocytosis and then moved to lysosomes. In comparison with pure liposomes, metal nanoparticles in the nanohybrids had little influence on the properties of liposomes. This study fills the gap of the function of nanoparticles in the overall nanohybrids, which provides a significant prerequisite for efficient drug delivery in therapeutic applications.

Keywords: liposomes, metal nanoparticles, nanohybrids, SERS, intracellular behavior.

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How to cite this article:
Zhu D, Wang Z, Zong S, Zhang Y, Chen C, Zhang R, Yun B, Cui Y. Investigating the Intracellular Behaviors of Liposomal Nanohybrids via SERS: Insights into the Influence of Metal Nanoparticles. Theranostics 2018; 8(4):941-954. doi:10.7150/thno.21173. Available from http://www.thno.org/v08p0941.htm