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Theranostics 2024; 14(3):1081-1097. doi:10.7150/thno.89190 This issue Cite

Research Paper

Quantitative visualization of myocardial ischemia-reperfusion-induced cardiac lesions via ferroptosis magnetic particle imaging

Wenwen Yang1,2,3†, Yueqi Wang3†✉, Changgeng Fu1,2†, Changjian Li4†, Feng Feng5, Hongzheng Li1,2,6, Ling Tan1,2, Hua Qu1,2, Hui Hui3, Jingjing Wang3,7✉, Jie Tian3✉, Linzi Long1,2,8✉

1. Department of Cardiology, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing, 100091, People's Republic of China.
2. National Clinical Research Center for Cardiovascular Diseases of Traditional Chinese Medicine, Beijing, 100091, People's Republic of China.
3. CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, the State Key Laboratory of Management and Control for Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, People's Republic of China.
4. School of Engineering Medicine, Beihang University, Beijing 100191, People's Republic of China.
5. College of Energy Engineering, Zhejiang university, Zhejiang 310058, People's Republic of China.
6. Beijing University of Traditional Chinese Medicine Graduate School, Beijing University of Chinese Medicine, Beijing, 100105, People's Republic of China.
7. Department of Cardiovascular Medicine, First Medical Center, General Hospital of the People's Liberation Army of China,Beijing, 100853, People's Republic of China.
8. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, People's Republic of China.
†These authors contributed equally to this work.

✉ Corresponding authors: Email address: yueqi.wangac.cn, newsjj2014com, jie.tianac.cn, qixiang830803com (Linzi Long, Tel: +86-010-62835035).

Citation:
Yang W, Wang Y, Fu C, Li C, Feng F, Li H, Tan L, Qu H, Hui H, Wang J, Tian J, Long L. Quantitative visualization of myocardial ischemia-reperfusion-induced cardiac lesions via ferroptosis magnetic particle imaging. Theranostics 2024; 14(3):1081-1097. doi:10.7150/thno.89190. https://www.thno.org/v14p1081.htm
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Abstract

Graphic abstract

Myocardial ischemia-reperfusion (MI/R) injury is a complication in vascular reperfusion therapy for MI, occurring in approximately 60% of patients. Ferroptosis is an important process in the development of MI/R cardiac lesions. Transferrin receptor 1 (TfR1), a marker of ferroptosis, corresponds to the changes in MI/R cardiac lesions and is expected to be a biomarker for detecting MI/R-induced ferroptosis. However, the noninvasive in vivo visualization of ferroptosis in MI/R is a big challenge. Thus, this study aimed to develop a novel multimodal imaging platform to identify markers of MI/R cardiac lesions in vivo through targeting TfR1.

Methods: Magnetic particle imaging (MPI) modality for ferroptosis based on superparamagnetic cubic-iron oxide nanoparticles (SCIO NPs), named feMPI, has been developed. FeMPI used TfR1 as a typical biomarker. The feMPI probe (SCIO-ICG-CRT-CPPs NPs, CCI NPs) consists of SCIO NPs, TfR1-targeting peptides (CRT), cell-penetrating peptides (CPPs), and indocyanine green (ICG). The specificity and sensitivity of CCI NPs in the MI/R mouse model were evaluated by MPI, magnetic resonance imaging (MRI), and near-infrared (NIR) fluorescent imaging.

Results: The intensity of the MPI signal correlates linearly with the percentage of infarct area in MI/R stained by TTC, enabling a quantitative assessment of the extent of cardiac lesions. Notably, these findings are consistent with the standard clinical biochemical indicators in MI/R within the first 24 h. FeMPI detects cardiac injury approximately 48 h prior to the current clinical imaging detection methods of MI/R.

Conclusion: The feMPI strategy can be a powerful tool for studying the process of MI/R-induced ferroptosis in vivo, providing clues for molecular imaging and drug development of ferroptosis-related treatments.

Keywords: myocardial ischemia-reperfusion, ferroptosis, magnetic particle imaging, quantitative, visualization

Citation styles

APA
Yang, W., Wang, Y., Fu, C., Li, C., Feng, F., Li, H., Tan, L., Qu, H., Hui, H., Wang, J., Tian, J., Long, L. (2024). Quantitative visualization of myocardial ischemia-reperfusion-induced cardiac lesions via ferroptosis magnetic particle imaging. Theranostics, 14(3), 1081-1097. https://doi.org/10.7150/thno.89190.

ACS
Yang, W.; Wang, Y.; Fu, C.; Li, C.; Feng, F.; Li, H.; Tan, L.; Qu, H.; Hui, H.; Wang, J.; Tian, J.; Long, L. Quantitative visualization of myocardial ischemia-reperfusion-induced cardiac lesions via ferroptosis magnetic particle imaging. Theranostics 2024, 14 (3), 1081-1097. DOI: 10.7150/thno.89190.

NLM
Yang W, Wang Y, Fu C, Li C, Feng F, Li H, Tan L, Qu H, Hui H, Wang J, Tian J, Long L. Quantitative visualization of myocardial ischemia-reperfusion-induced cardiac lesions via ferroptosis magnetic particle imaging. Theranostics 2024; 14(3):1081-1097. doi:10.7150/thno.89190. https://www.thno.org/v14p1081.htm

CSE
Yang W, Wang Y, Fu C, Li C, Feng F, Li H, Tan L, Qu H, Hui H, Wang J, Tian J, Long L. 2024. Quantitative visualization of myocardial ischemia-reperfusion-induced cardiac lesions via ferroptosis magnetic particle imaging. Theranostics. 14(3):1081-1097.

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