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Theranostics 2024; 14(3):1101-1125. doi:10.7150/thno.91362 This issue Cite

Review

Construction of tumor organoids and their application to cancer research and therapy

Jiajing Lv1,2,3, Xuan Du4, Miaomiao Wang1,2,5, Jiacan Su1,3,6✉, Yan Wei1,3✉, Can Xu7✉

1. Institute of Translational Medicine, Shanghai University, Shanghai 200444, China.
2. Institute of Medicine, Shanghai University, Shanghai 200444, China.
3. Organoid Research Center, Shanghai University, Shanghai 200444, China.
4. Biopharma Industry Promotion Center Shanghai, Shanghai 201203, China.
5. Department of Rehabilitation Medicine, Shanghai Zhongye Hospital, Shanghai, 200941, China.
6. Department of Orthopedics, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
7. Department of Gastroenterology, Changhai Hospital, Naval Medical University, Shanghai 200433, China.

✉ Corresponding authors: Jiacan Su, drsujiacancom; Yan Wei, yweiedu.cn; Can Xu, xucanedu.cn.

Citation:
Lv J, Du X, Wang M, Su J, Wei Y, Xu C. Construction of tumor organoids and their application to cancer research and therapy. Theranostics 2024; 14(3):1101-1125. doi:10.7150/thno.91362. https://www.thno.org/v14p1101.htm
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Abstract

Graphic abstract

Cancer remains a severe public health burden worldwide. One of the challenges hampering effective cancer therapy is that the existing cancer models hardly recapitulate the tumor microenvironment of human patients. Over the past decade, tumor organoids have emerged as an in vitro 3D tumor model to mimic the pathophysiological characteristics of parental tumors. Various techniques have been developed to construct tumor organoids, such as matrix-based methods, hanging drop, spinner or rotating flask, nonadhesive surface, organ-on-a-chip, 3D bioprinting, and genetic engineering. This review elaborated on cell components and fabrication methods for establishing tumor organoid models. Furthermore, we discussed the application of tumor organoids to cancer modeling, basic cancer research, and anticancer therapy. Finally, we discussed current limitations and future directions in employing tumor organoids for more extensive applications.

Keywords: tumor organoids, cellular components, culture methods, basic cancer research, antitumor therapy

Citation styles

APA
Lv, J., Du, X., Wang, M., Su, J., Wei, Y., Xu, C. (2024). Construction of tumor organoids and their application to cancer research and therapy. Theranostics, 14(3), 1101-1125. https://doi.org/10.7150/thno.91362.

ACS
Lv, J.; Du, X.; Wang, M.; Su, J.; Wei, Y.; Xu, C. Construction of tumor organoids and their application to cancer research and therapy. Theranostics 2024, 14 (3), 1101-1125. DOI: 10.7150/thno.91362.

NLM
Lv J, Du X, Wang M, Su J, Wei Y, Xu C. Construction of tumor organoids and their application to cancer research and therapy. Theranostics 2024; 14(3):1101-1125. doi:10.7150/thno.91362. https://www.thno.org/v14p1101.htm

CSE
Lv J, Du X, Wang M, Su J, Wei Y, Xu C. 2024. Construction of tumor organoids and their application to cancer research and therapy. Theranostics. 14(3):1101-1125.

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