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Theranostics 2024; 14(5):2232-2245. doi:10.7150/thno.94121 This issue Cite

Research Paper

Dynamic pathological analysis reveals a protective role against skin fibrosis for TREM2-dependent macrophages

Yunsheng Liang^1,2#, Yongfei Hu^1#, Jun Zhang^1#, Haosen Song¹, Xiaoqian Zhang¹, Yishan Chen¹, Yu Peng¹, Lihua Sun³, Yuzhe Sun¹, Ruzeng Xue², Suyun Ji², Chuanwei Li¹, Zhili Rong¹, Bin Yang^1,2✉, Yingping Xu^1✉

1. Institute of Dermatology and Venereology, Dermatology Hospital, Southern Medical University, Guangzhou 510091, China.
2. Department of Dermatology, Dermatology Hospital, Southern Medical University, Guangzhou 510091, China.
3. Department of Gynecology and Obstetrics, Nanhai Hospital, Southern Medical University, Guangzhou 528200, China.
# These authors contributed equally to this work.

✉ Corresponding authors: Institute of Dermatology and Venereology, Dermatology Hospital, Southern Medical University. E-mail: XuYP01edu.cn (Y. P. Xu); Yangbin1edu.cn (B. Yang).

Abstract

Rationale: Systemic sclerosis (SSc) is a chronic and incurable autoimmune disease with high mortality rates, and skin fibrosis is one of distinguishing hallmarks in the pathogenesis. However, macrophage heterogeneity regulating skin fibrosis remain largely unknown.

Methods: We established mouse disease model and performed single-cell RNA-sequencing (scRNA-seq) to resolve the dynamic and heterogenous characteristics of macrophages in skin fibrosis, and the role of TREM2-dependent macrophages in the pathological process was investigated using knockout mice and intraperitoneal transferring TREM2⁺ macrophages combining with functional assays.

Results: We show that TREM2-expressing macrophages (TREM2⁺ MФs) accumulate in injured skin of mice treated by bleomycin (BLM) and human SSc, and their gene signatures and functional pathways are identified in the course of disease. Genetic ablation of Trem2 in mice globally accelerates and aggravates skin fibrosis, whereas transferring TREM2^hi macrophages improves and alleviates skin fibrosis. Amazingly, we found that disease-associated TREM2⁺ MФs in skin fibrosis exhibit overlapping signatures with fetal skin counterparts in mice and human to maintain skin homeostasis, but each has merits in skin remodeling and development respectively.

Conclusion: This study identifies that TREM2 acts as a functional molecule and a major signaling by which macrophage subpopulations play a protective role against fibrosis, and disease-associated TREM2⁺ MФs in skin fibrosis might undergo a fetal-like reprogramming similar to fetal skin counterparts.

Keywords: Skin fibrosis, macrophages, TREM2, single cell RNA sequencing

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