Senescent melanocytes driven by glycolytic changes are characterized by melanosome transport dysfunction

Park, Young Joon; Kim, Jin Cheol; Kim, Yeongeun; Kim, Yul Hee; Park, Soon Sang; Muther, Charlotte; Tessier, Agnès; Lee, Gimyung; Gendronneau, Gaëlle; Forestier, Sandra; Ben-Khalifa, Youcef; Park, Tae Jun; Kang, Hee Young

doi:10.7150/thno.84912

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Nanotheranostics 2024; 8(4): 442-457. [Abstract] [Full text] [PDF]

Nanotheranostics 2024; 8(4): 427-441. [Abstract] [Full text] [PDF]

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Theranostics 2023; 13(12):3914-3924. doi:10.7150/thno.84912 This issue Cite

Research Paper

Senescent melanocytes driven by glycolytic changes are characterized by melanosome transport dysfunction

Young Joon Park^1*, Jin Cheol Kim^1*, Yeongeun Kim^1,4, Yul Hee Kim¹, Soon Sang Park^3,4, Charlotte Muther², Agnès Tessier², Gimyung Lee², Gaëlle Gendronneau², Sandra Forestier², Youcef Ben-Khalifa², Tae Jun Park^3,4✉, Hee Young Kang^1,4✉

1. Department of Dermatology, Ajou University School of Medicine, Suwon, Korea.
2. Innovation Research and Development, Chanel Parfums Beauté, Pantin, France.
3. Department of Biochemistry and Molecular Biology, Ajou University School of Medicine, Suwon, Korea.
4. Inflamm-Aging Translational Research Center, Ajou University School of Medicine, Suwon, Korea.
*These authors contributed equally to this work and share first authorship.

Citation:

Park YJ, Kim JC, Kim Y, Kim YH, Park SS, Muther C, Tessier A, Lee G, Gendronneau G, Forestier S, Ben-Khalifa Y, Park TJ, Kang HY. Senescent melanocytes driven by glycolytic changes are characterized by melanosome transport dysfunction. Theranostics 2023; 13(12):3914-3924. doi:10.7150/thno.84912. https://www.thno.org/v13p3914.htm

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Abstract

Rationale: Senescent melanocytes accumulate in photoaged skin and are closely related to skin aging. A better understanding of the molecular characteristics of senescent melanocytes may be the key to controlling skin aging.

Methods: We have developed an in vitro model of senescence in melanocytes using UV irradiation and investigated the functional characteristics and molecular mechanisms underlying senescence in UV-irradiated melanocytes.

Results: We have highlighted that in vitro senescent melanocytes are characterized by melanosome transport dysfunction resulting in melanin accumulation. The defective melanosome transport was confirmed with the ultrastructural characterization of both in vitro UV-induced senescent melanocytes and in vivo melanocytes of hypopigmented aging skin. A single-cell transcriptomic analysis revealed that the glycolytic metabolism pathway appeared to be significantly upregulated in most senescent phenotypes. Furthermore, the inhibition of glycolysis by pharmacological compounds mitigates the pro-aging effects of melanocytes senescence, suggesting that alterations in cellular glucose metabolism act as a driving force for senescence in melanocytes.

Conclusion: These results demonstrate that senescent melanocytes are characterized by glycolytic metabolism changes and a defective melanosome transport process, which may be related to impaired mitochondrial function, highlighting the importance of metabolic reprogramming in regulating melanocyte senescence.

Keywords: glycolysis, melanocyte, melanosome, senescence, single-cell RNA sequencing, photoaging

Citation styles

APA

Park, Y.J., Kim, J.C., Kim, Y., Kim, Y.H., Park, S.S., Muther, C., Tessier, A., Lee, G., Gendronneau, G., Forestier, S., Ben-Khalifa, Y., Park, T.J., Kang, H.Y. (2023). Senescent melanocytes driven by glycolytic changes are characterized by melanosome transport dysfunction. Theranostics, 13(12), 3914-3924. https://doi.org/10.7150/thno.84912.

ACS

Park, Y.J.; Kim, J.C.; Kim, Y.; Kim, Y.H.; Park, S.S.; Muther, C.; Tessier, A.; Lee, G.; Gendronneau, G.; Forestier, S.; Ben-Khalifa, Y.; Park, T.J.; Kang, H.Y. Senescent melanocytes driven by glycolytic changes are characterized by melanosome transport dysfunction. Theranostics 2023, 13 (12), 3914-3924. DOI: 10.7150/thno.84912.

NLM

CSE

Park YJ, Kim JC, Kim Y, Kim YH, Park SS, Muther C, Tessier A, Lee G, Gendronneau G, Forestier S, Ben-Khalifa Y, Park TJ, Kang HY. 2023. Senescent melanocytes driven by glycolytic changes are characterized by melanosome transport dysfunction. Theranostics. 13(12):3914-3924.

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