Theranostics 2011; 1:189-200. doi:10.7150/thno/v01p0189 This volume Cite


Integrin Targeted Delivery of Chemotherapeutics

Kai Chen1,2 Corresponding address, Xiaoyuan Chen 1 Corresponding address

1. National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, MD;
2. Molecular Imaging Center, Department of Radiology, Keck School of Medicine, University of Southern California, Los Angeles, CA

Chen K, Chen X. Integrin Targeted Delivery of Chemotherapeutics. Theranostics 2011; 1:189-200. doi:10.7150/thno/v01p0189.
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Targeted delivery of chemotherapeutics is defined in the sense, that is, to maximize the therapeutic index of a chemotherapeutic agent by strictly localizing its pharmacological activity to the site or tissue of action. Integrins are a family of heterodimeric transmembrane glycoproteins involved in a wide range of cell-to-extracellular matrix (ECM) and cell-to-cell interactions. As cell surface receptors, integrins readily interact with extracellular ligands and play a vital role in angiogenesis, leukocytes function and tumor development, which sets up integrins as an excellent target for chemotherapy treatment. The peptide ligands containing the arginine-glycine-aspartic acid (RGD), which displays a strong binding affinity and selectivity to integrins, particularly to integrin αvβ3, have been developed to conjugate with various conventional chemotherapeutic agents, such as small molecules, peptides and proteins, and nanoparticle-carried drugs for integtrin targeted therapeutic studies. This review highlights the recent advances in integrin targeted delivery of chemotherapeutic agents with emphasis on target of integrin αvβ3, and describes the considerations for the design of the diverse RGD peptide-chemotherapeutics conjugates and their major applications.

Keywords: Integrin, Targeted delivery, Chemotherapeutics, RGD, Bioconjugation.


Chemotherapy has been the main approach for the systemic treatment of advanced or metastatic diseases for more than half century. During the cells division process, chemotherapeutics is capable of killing proliferating cells, hence affecting aberrant cells. While highly efficient in preventing disease progression, however, chemo-therapeutic agents are often lack of selectivity for aberrant cells. Their toxicity to normal cells and non-diseased tissues, especially to rapidly growing cells such as blood, bone marrow and mucous membrane cells, cause serious side-effects that impose to administrate the chemotherapeutic agents at sub-optimal doses [1]. Because of intrinsic or acquired resistance of aberrant cells, benefits of the chemotherapeutic agents are also often limited [2]. The efficiency of the treatment can be increased by escalating the doses, but this option commonly results in considerable toxicity problem and thus is rarely considered. Selective delivery of chemotherapeutic agents to the disease site therefore represents a major challenge for improving current chemotherapy outcome [3]. Targeted delivery of chemotherapeutics is an emerging therapy strategy endowed with disease-targeting functions and carrying cytotoxic components by enabling the specific delivery of chemotherapeutic agents to aberrant tissues, thereby increasing their local efficacy while limiting their peripheral toxicity. Clearly, the success of this approach is heavily on the rational selection of appropriate biological targets.

Integrin - An ideal therapeutic target

In the past two decades, many efforts have been made to the discovery of cell adhesion molecules (CAMs), and their distinctive functions in cell morphology, locomotion, mitosis, cytokinesis, phagocytosis, and the maintenance of cell polarity [4-6]. It is known that CAMs play important roles in various disease states such as cancer [7-9], thrombosis [10-11], rheumatoid arthritis [12], and diabetes [13]. Numerous studies have been actively performing in investigation of the structure, function, and recycling mechanisms of some CAMs, as well as how to modulate them for controlling disease progression [14]. CAMs are glycoproteins expressed on the cell surface that functionalize as receptors for cell-to-cell and cell-to-extracellular matrix (ECM) adhesion [15-17]. In general, CAMs can be divided into four classes: integrins, selectins, cadherins, and the immunoglobulin superfamily.

Among these CAMs, integrins are a family of heterodimeric transmembrane glycoproteins involving in a wide range of cell-to-ECM and cell-to-cell interactions [18-19]. Each member of this family consists of non-covalently bound transmembrane polypeptide α and β subunits. In mammals, 18 α and 8 β subunits have been identified, which assemble into at least 24 different integrins [20]. The term integrin is derived from the ability of these proteins to link the ECM proteins with the intracellular cytoskeleton [21]. During binding to ECM proteins, integrins form clusters at the cell membrane that are associated with a cytoskeletal complex to promote actin filament assembly [22-23]. In turn, the reorganization of actin filaments into large stress fibers increases integrin clustering followed by enhancement of binding affinity with ECM proteins [24]. During cell locomotion and migration, integrin can undergo endocytosis and exocytosis [25-27]. In the process of cell locomotion, integrins activation can be triggered by vascular endothelial growth factor (VEGF) and controlled by protein kinase C (PKC) to continuously form new integrin-based focal contacts at the front of the cell [28]. While the cell migrates, the integrin interaction is released from extracellular ligands at the rear of the cells after forming new and persistent integrin connections at the front of the cell [29-30]. In migrating neutrophils, the distribution of integrins was observed to be maintained by Ca2+-dependent release of cell adhesion followed by endocytosis of the integrin [31]. Thus, integrin endocytosis has been considered as an attractive mechanism for controlling cell signaling pathways which can be stimulated by ligand binding.

The characteristic that integrins can be internalized by cells on activation with anchoring ligands significantly facilitates delivery of chemotherapeutics into neoplastic cells and leukocytes, while these chemotherapeutics conjugate with integrin-targeted ligands. Additionally, integrins are over-expressed on angiogenetic endothelial cells, whereas they are absent in pre-existing endothelial cells and normal tissues. Moreover, it has been found that certain tumor cells over-express integrins on their cellular membrane surface [32-34], which makes selective delivery of chemotherapeutic agents possible. All of these characteristics set up integrin an ideal target for both cancer and anti-angiogenesis therapy.

Integrin and RGD-peptide

The arginine-glycine-aspartic acid (RGD) cell adhesion sequence was discovered in fibronectin over two decades ago [35-36]. Later on, RGD-recognition sites were found in other ECM proteins [37-40] and the receptors for these ECM proteins were identified in the integrin family. Proteins that contain the RGD attachment site, together with the integrins that serve as receptors for them, constitute a major recognition system for cell adhesion. The RGD sequence is the cell attachment site of a number of adhesive ECM, blood, and cell surface proteins. It has been found that nearly half of the over 20 known integrins, including all five αv integrins, two β1 integrins (α5, α8) and αIIbβ3 integrin, recognize this sequence in their adhesion protein ligands [41].

The integrin αvβ3, consisting a 125-kDa αv subunit and a 105-kDa β3 subunit, binds a wide range of ECM proteins with RGD-containing components of the interstitial matrix such as vitronectin, fibronectin and thrombospondin [42-43]. Crystal structures of integrin αvβ3 complexed with RGD ligands have revealed an atomic basis for this interaction [43]. RGD binds at an interface between the α and β subunits, the R residue fitting into a cleft in a β-propeller module in the subunit, and the D coordinating a cation bound in a von Willebrand factor A - domain in the β subunit. It is well documented that Integrin αvβ3 is expressed on the cell membrane of various tumor cell types such as late stage glioblastoma, melanoma, ovarian, breast, and prostate cancer [32-34]. Integrin αvβ3 also plays critical role in tumor invasion and metastasis arising from its ability to recruit and activate MMP-2 and plasmin, which can degrade components of the basement membrane and interstitial matrix [44]. Among integrins family, integrin αvβ3 is the most intensively studied although many other integrins such as αvβ1, αvβ5, α5β1, and α4β1 also play important roles in regulating angiogenesis [45-49].

Based on these findings, linear as well as cyclic RGD peptides have been developed as ligands that preferentially bind to integrin αvβ3, related αv integrins, or other types of integrins. Among these RGD peptides, cyclic peptides c(RGDfK) and c(RGDyK), RGD4C, and RGD10 (Figure 1) showed high binding affinity and selectivity for integrin αvβ3 [50]. Thus, these RGD-peptides can be served as vectors for integrin αvβ3 targeted delivery of chemotherapeutics. Cyclic peptides c(RGDfK) and c(RGDyK) are the ones mostly used for the delivery of therapeutic agents. The amino group of the lysine residue on these peptides is an ideal site for further chemical conjugation reactions. Multivalent c(RGDfK) or c(RGDyK) peptide can be used to achieve higher binding affinity. Both of RGD4C and RGD10 peptide were discovered by phage display technology [51-52]. The RGD4C peptide contains two disulfide bonds and displays at least 20-fold more potent than similar peptide with a single disulfide bond. Although the RGD10 peptide contains only one disulfide bond, the residues flanking the CRGDC core display similar physicochemical properties as those in RGD4C. Overall, RGD4C and RGD10 show similar binding affinity to integrin αvβ3.

To date, agents that are commonly used to conjugate with RGD-peptides for chemotherapy treatments are: a) therapeutic small molecules, b) therapeutic peptides and proteins, and c) nanoparticle-carried chemotherapeutics.

 Fig 1 

Chemical Structures of c(RGDyK), c(RGDfK), [c(RGDyK)2], RGD4C, and RGD10. Due to high binding affinity for integrin αvβ3, these RGD-containing peptides are commonly used for integrin αvβ3 targeted delivery of chemotherapeutics.

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Integrin targeted delivery of therapeutic small molecules

The first approach in which the RGD-peptide is used for drug targeting purposes focuses on the delivery of cytotoxic drugs for cancer treatments. These treatments are often effective, however, their high toxicity to healthy tissues is difficult to tolerate for patients. Thus, development of alternative vectorization of the cytotoxic drugs without killing healthy cells is critically demanded to circumvent this side effect.

For instance, doxorubicin, commonly used anticancer drug, was conjugated with RGD-peptides for integrin targeted therapy. A doxorubicin-RGD4C conjugate (doxo-RGD4C) is one of good examples. Doxo-RGD4C demonstrated equal efficacy as free doxorubicin in vitro and, more importantly, doxo-RGD4C showed improved inhibition of tumor growth and spreading of pulmonary metastases than free doxorubicin in mouse MDA-MB-435 breast cancer model, in which integrin αvβ3 is expressed by the endothelium in the angiogenic blood vessels and by the tumor cells themselves [53]. In addition, doxo-RGD4C was also found to be less toxic to liver and heart. In another similar study, doxo-RGD4C was tested in mouse αvβ3-negative MH134 hepatoma tumor model. As compared to doxorubicin alone, the doxo-RGD4C conjugate showed less treatment efficacy in vitro; however, the doxo-RGC4C conjugate demonstrated better anti-tumor activity in vivo. Because direct anti-tumor cell effects of the targeted doxorubicin are not expected in this integrin αvβ3-negative tumor model, this study suggests the anti-angiogenetic effect on the endothelial cells induced by doxo-RGD4C conjugate may lead to tumor recession [54].

One of the current strategies for providing substantial increases in the clinical efficacy of chemotherapeutic agents that preferentially kill dividing cells, both normal and tumor, is the use of relatively nontoxic prodrug forms that can be selectively activated in aberrant tissue [55]. Prodrugs are generally defined as agents that are transformed after administration, either by metabolism or spontaneous chemical breakdown, to pharmacologically active species. Several doxo-RGD4C conjugates have been designed as integrin αvβ3 targeted prodrugs that require activation by tumor-secreted enzymes. This strategy would reduce the toxicity of drugs and allow higher doses to be systemically delivered than non-targeted chemotherapeutic agents. A prodrug containing a plasmin-specific cleavage site was developed by de Groot et al. [56]. The release of the parent drug - doxorubicin can be achieved by plasmin cleavage followed by accumulation of the prodrug onto integrin αvβ3. In vitro studies showed the promising activity of prodrug after incubation with plasmin. The RGD-doxo prodrug was further proved to be capable of blocking the adhesion of endothelial cells to vitronectin in submicromolar concentrations, suggesting the prodrug retained antiangiogenic activity. However, poor solubility of doxo-RGD4C prodrug as well as difficulty of synthesizing RGD4C-drug conjugates are problematic for further development of this type of agents [54, 56-57]. In addition, two disulfide bonds in the RGD4C peptide are susceptible to be disrupted under reduced condition in cytoplasm, resulting in inactive form. In a recent study, Burkhart et al. [57] designed two RGD-based (RGD4C and Cilengitide) conjugates with the doxsaliform prodrug, which is spontaneously converted into an active metabolite of doxorubicin, doxorubicinformaldehyde, and leads to more cytotoxicity than doxorubicin [58]. Both conjugates maintained high affinity to integrin αvβ3 with IC50 values of 5-10 nanomolar. The in vitro cytotoxicity studies also revealed good growth inhibition of MDA-MB-435 breast cancer cells, demonstrating their capability of binding to integrin αvβ3. Further experiments showed that the RGD-containing prodrugs cannot significantly penetrate the cell membrane, in contrast to native doxorubicin and the released doxorubicin-formaldehyde. Thus, the proposed mechanism of action of these conjugates is to bind to integrin αvβ3 initially, followed by local release of the more lipophilic doxorubicin species, which then diffuse through cell membranes. Unfortunately, this study only focused on in vitro stage. In vivo anti-tumor efficacy of these two conjugates was not reported.

Besides doxorubicin, several other chemotherapeutic small molecules were also conjugated with RGD-peptides. We recently evaluated the antitumor activity of paclitaxel conjugated with a dimeric RGD peptide E[c(RGDyK)]2 (RGD2) [59]. Paclitaxel (PTX), a prototype of the taxane family of antitumor drugs, is commonly used in the treatment of advanced metastatic breast cancer. The RGD2-PTX conjugate inhibited cell proliferation with activity in comparable with that observed for paclitaxel, both of which are mediated by an arrest of G2/M phase of the cell cycle followed by apoptosis. We then labeled RGD2-PTX with 125I through the tyrosine residue on the RGD peptide. Integrin-specific accumulation of 125I-RGD2-PTX in orthotopic MDA-MB-435 tumor was observed. In a follow-up study, we evaluated the in vivo anti-tumor effect of the RGD2-PTX conjugate [60]. The treatment efficacy of RGD2-PTX was confirmed by size measurement, in vivo PET imaging, and ex vivo histopathology. The tumor growth delay is related to tumor proliferation rather than tumor metabolism, as confirmed by [18F]FDG and [18F]FLT PET imaging.

Kok's group recently developed a novel linking technique for the conjugation of drug to carriers by applying platinum-coordination chemistry [61]. The so-called universal linker system (ULS) can release the drug via a slow release profile. Thus, a prolonged action of the delivered drug can be achieved. New classes of drug carriers consisted human serum albumin (HSA), cyclic RGD peptides, and polyethylene glycol (PEG). A kinase inhibitor PTK787 binds to the ULS through a coordination linkage at one of the aromatic nitrogen atoms. Drug-targeting conjugates and the respective control conjugates were analyzed for binding affinity to the integrin αvβ3 target receptor on human umbilical vein endothelial cells (HUVEC). All RGD-equipped conjugates displayed reasonable binding affinity to integrin αvβ3, suggesting the conjugated PTK787 did not obstruct binding of the RGD-modified carriers to integrin αvβ3. The highest binding affinity was observed for RGD-PTK787-HSA (IC50 = 4.4 nM) followed by RGD-PTK787-HSA-PEG (IC50 = 65 nM) and RGD-PEG-PTK787-HSA (IC50 = 640 nM). The result implies the additional incorporation of PEG partially obstructed binding in vitro; however, in vivo effect of the PEG moiety for these particular conjugates keeps unknown. Kok's group also developed a series of closely related drug targeting conjugates, consisting of albumins equipped with RGD-peptide, PEG stealth domains, and either the antitubulin agent monomethyl auristatin E (MMAE) or a new F-variant (MMAF) [62-63]. Since MMAF has a C-terminal charge, this compound is potentially less prone to passive redistribution after its release from the carrier. The results demonstrated that RGD-equipped albumin conjugates with MMAF were more potent than MMAE conjugates, in killing both integrin αvβ3-positive tumor cells and proliferating endothelial cells. Efficacy increased more in tumor cells than in endothelial cells, suggesting different drug redistribution behavior for the two cell types. However, in vivo studies need to be performed to further evaluate in vivo efficacy and toxicity of these RGD-drug conjugates.

The various conjugates of RGD-peptides and therapeutic small molecules [53-54,56,57,59,61-66] are summarized in Table 1.

 Table 1 

Integrin targeted delivery of therapeutic small molecules

Therapeutic agentTargeting motifExperimental modelResultsaRef.
In VitroIn Vivo
Doxorubicin (Dox)RGD4CMDA-MB-435 mammary carcinoma++[53]
DoxorubicinRGD4CMH134 murine hepatoma-+[54]
Plasmin cleavable prodrugRGD4CHUVEC=ND[56]
Doxsaliform (doxorubicinformaldehyde)c[RGDf(N-Me)V]MDA-MB-435=ND[57]
DoxorubicinRGD-containing tetrameric peptideH2009 and H1299+ND[64]
Arabinoside CRGDSKB16 melanoma-=[66]
PTK787 albuminc(RGDfK)-PEGHUVEC+ND[61]
MMAE (Auristatin E)c(RGDfK)-PEGC26 murine colon carcinoma++[65]
MMAF (Auristatin F)c(RGDfK)-PEGC26 murine colon carcinoma+ND[62]
SB202190 (the p38 MAPkinase Inhibitor)c(RGDfK)-PEGHUVEC+ND[63]

a Efficacy compared to non-targeted chemotherapeutic agent: Improved efficacy (+), comparable efficacy (=), less efficacy (-), and not determined (ND).

Integrin targeted delivery of therapeutic peptide and proteins

The second approach relies on the conjugation of integrin ligands with therapeutic biologically relevant biomolecules, such as peptides and proteins, to target them to the specific site for their action. A good number of therapeutic peptides and proteins are currently becoming available for the treatment of cancer and other diseases. However, poor pharmacokinetics and unacceptable side-effects are often limiting the efficacy of these biomolecules. For instance, cytokines show potent activity in various cancers, but they normally display a range of other actions within the body, which may cause severe adverse events. Cell-specific targeting of a cytokine may significantly improve its selectivity for the target cells within the tumor and, thus, enhance its efficacy. In general, biomolecules, such as therapeutic peptides and proteins, are hydrophilic and they have short plasma half-life. In order to achieve favorable pharmacokinetic properties of therapeutic biomolecules, structural optimizations of RGD-peptides by using PEGylation strategy sometimes are necessary, which could potentially prevent fast renal clearance, enzymatic degradation of the biomolecules, and extend therapeutic windows.

The conjugate of RGD4C peptide with a pro-apoptotic cationic peptide (KLAKLAK)2 has been reported [67]. The in vitro results showed RGD4C-(KLAKLAK)2 induced apoptosis in Kaposi sarcoma tumor cells. In vivo studies, RGD4C-(KLAKLAK)2 reduced tumor weight and size as well as diminish the formation of lung metastases. In addition, intravenous administration of RGD4C-(KLAKLAK)2 to mice subjected to collagen-induced arthritis significantly reduced the arthritic score [68], which is attributed by apoptosis of endothelial cells in the inflamed synovium. This study demonstrated that targeting to integrin αvβ3-positive endothelial cells is not only limited to the treatment of cancer, but also can be a feasible therapeutic approach for chronic inflammatory disease, in which angiogenesis is a hallmark of disease progression as well. In another study [69], RGD4C peptide was conjugated with a naturally occurring antimicrobial cationic peptide, tachyplesin. The in vitro results showed that RGD4C-tachyplesin inhibited the proliferation of both cultured tumor and endothelial cells and reduced the colony formation of TSU prostate cancer cells. The in vivo studies indicated that the RGD4C-tachyplesin could inhibit the growth of tumors on the chorioallantoic membranes of chicken embryos and in syngeneic mice.

Tumor necrosis factor α (TNF-α) is a multi-functional cytokine playing a key role in apoptosis and cell survival, as well as in inflammation and immunity. Although TNF-α treatment on tumor progression shows beneficial effects in animal studies, clinical use of TNF-α in treatment of cancer is limited due to systemic toxicity. It has been found that the full potential of TNF-α could be exploited while systemic distribution of TNF-α was prevented by local treatment protocols such as isolated limb perfusion [70-71]. Thus, specific targeting of TNF-α to a selective cell population may improve its cytostatic potential. One example is the conjugate of RGD peptide with the TNF mutant V29, where Arginine 29 was replaced by Valine. TNF-V29 selectively binds to TNF-R1 rather than TNF-R2 [72], and TNF-V29 showed potent antitumor activity in mice, combined with a reduced systemic toxicity [73]. In addition, it has been reported that RGD-motif in wild-type TNF reduced its gastrointestinal toxicity [74] and improved its anti-metastatic activity [75]. Thus, the RGD-TNF-V29 mutant F4614 was expected to achieve better overall therapeutic efficacy as compared to the RGD and wild-type TNF conjugate. Indeed, RGD-TNF-V29 displayed considerably lower cytotoxicity in normal endothelial cells, while culturing in tumor-conditioned medium restored the TNF-responsiveness of endothelial cells to the level of wild type TNF-α [76]. RGD-TNF-V29 also showed an increased tumor selectivity of up to 460-fold, whereas wild-type TNF displayed only a 4-fold difference in tumor-endothelial selectivity. In in vivo studies, RGD-TNF-V29 demonstrated a 2-fold improved therapeutic index (LD50/MED = 4.8), which may be contributed by the improved accumulation in the tumor tissue [73]. In another example [77], RGD4C peptide was conjugated with a TNF-α fusion protein, mouse-TNF-α (mTNF-α). Because mTNF is a compact homotrimeric protein, RGD4C-TNF actually contains three binding domains per protein. Due to this multivalent effect, RGD4C-TNF showed a 10-fold higher affinity than free RGD4C peptide for integrin αvβ3 in the endothelial cell line EA.hy926. It was also found that subnanogram doses of RGD4C-TNF are sufficient to induce antitumor effects in tumor-bearing mice as combined with melphalan, a chemotherapeutic drug. Cell adhesion assays and competitive binding experiments with anti-integrin antibodies further demonstrated that showed that the RGD moiety interacts with integrin αvβ3. In addition, RGD4C-TNF conjugate induced cytotoxic effects in standard cytolytic assays, implying that RGD4C-TNF conjugate can also bind TNF receptors and trigger death signals. These results suggested that coupling TNF with RGD4C improves its anti-neoplastic activity. We recently developed an RGD4C-TNF fusion protein for tumor-specific delivery of TNF [78-79]. RGD4C-TNF was significantly more potent than TNF in inhibiting orthotopic MDA-MB-435 tumor growth. Intramuscular administration of plasmid DNA encoding RGD4C-TNF also was found to inhibit the growth of melanomas and lymphomas implanted at sites distant from the plasmid injection site [80]. Tumor necrosis factor (TNF)-related apoptosis inducing ligand (TRAIL) is an apoptosis-inducing member of the TNF gene family, which acts as a homotrimer interacting with five cognate receptors: TRAIL-R1, TRAIL-R2, TRAIL-R3, TRAIL-R4, and osteoprotegerin (OPG). Unlike the other TNF family members TNF-α and CD95L, TRAIL has the unique ability to elicit apoptotic cell death in a variety of cancer cells, with minimal cytoxicity for normal cells and tissues. The conjugate of the fusion of TRAIL with the RGD-based peptide has been reported [81]. The results showed that RGD4C led to enhanced apoptosis inducing activity (caspase-3 and caspase-8 activation) in integrin αvβ3 and αvβ5-positive cancer cells.

Tissue Factor (TF) is a cell membrane receptor protein that is the initiator of the extrinsic pathway of the blood coagulation cascade and normally released from damaged tissues [82]. It is expected that this potent thrombogenic protein in its truncated form (tTF) can be targeted to the tumor, occlude the tumor's blood supply and, thus cause rapid tumor destruction. To test this hypothesis, three fusion proteins, chTNT-3/tTF, chTV-1/tTF, and RGD/tTF, which target DNA exposed in degenerative areas of tumors, fibronectin on the tumor vascular basement membrane, and integrin αvβ3 on the luminal side of tumor vessels, respectively, were developed and tested for their antitumor effects [82]. In vitro, all fusion proteins retained similar thrombotic activity. In MAD109 mouse lung tumor model, RGD-tTF was found to be localized mainly in capillaries and small vessels of the tumor. In vivo, daily injections of RGD-tTF resulted in thrombosis of about 40% of the tumor blood vessels, but no significant inhibition of tumor growth was observed. In contrast, the other two fusion proteins showed thrombosis in up to 80% of the scored blood vessels, leading to massive tumor necrosis and more than 50% reduction in tumor volume versus the RGD-tTF group. Similar results were obtained in the C26 colon carcinoma model. The data implied that these thrombogenic agents had to occlude medium and large vessels within the tumor in order to attain a significant antitumor effect. Interestingly, most impressive tumor suppression was observed for the combination therapy of all three-fusion proteins, suggesting the delivery of tTF to all available targets produced an additive thrombotic effect.

Interleukin 12 (IL-12) plays an important role in the activities of natural killer (NK) cells and T lymphocytes. It mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. However, systemic administration of IL-12 was associated with dose-limiting toxicity, thus preventing IL-12 from attaining its full therapeutic potential [83]. A fusion protein was synthesized by conjugating mouse IL-12 with RGD4C [84]. The results showed that RGD4C-IL-12 retained the immunostimulatory activity of IL-12. In corneal angiogenesis assay, RGD4C-IL-12 demonstrated excellent inhibition of bFGF-induced vessel growth, whereas native mIL-12 only partially inhibited neovascularization. In addition, in a neuroblastoma model (NXS2 model), RGD4C-IL-12 showed an improved antitumor effect, whereas native IL-12 was not effective. Moreover, RGD4C-mediated targeting prevented IL-12 induced hepatic necrosis, which was observed after continuous subcutaneous infusion for two weeks via surgically implanted osmotic pumps. While RGD-IL-12 was tested in knockout mice lacking the IL-12 receptor, RGD4C-IL-12 showed the neovascularization inhibition for up to 25%, whereas mIL-12 was completely ineffective. The enhanced antiangiogenic effect of mrIL-12vp may involve several mechanisms, including increased IL-12 concentrations delivered directly to angiogenic endothelial cells, activation of immune cells within the angiogenic site, and contribution of RGD4C in suppressing endothelial cell survival pathways.

The various conjugates of RGD-peptides and therapeutic peptides and proteins [67,69,73,77-78,80-82,84-88] are summarized in Table 2.

 Table 2 

Integrin targeted delivery of therapeutic peptides and proteins

Therapeutic agentTargeting motifExperimental modelResultsaRef.
In VitroIn Vivo
(KLAKLAK)2RGD4CMDA-MB-435 mammary carcinoma++[67]
TachyplesinCRGDCTSU prostate cancer cell lines and B16 melanoma++[69]
Truncated tissue factor (tTF)RGD4CMAD109 and COLON 26++[82]
mIL12RGD4CNXS2 neuroblastoma-+[84]
EndostatinRGDSLS174T colon carcinoma++[85]
Fc fragment of IgGRGD4CDU145 prostate carcinomaND+[86]
Hu-TNFαRGD4CB16F1 melanomaND+[80]
Hu-TNFα mutant selective for TNFR1 (V29)RGDSDMeth A fibrosarcoma and Mqnu-1 lung cancer-+[73]
TRAILc(RGDfK)-PEGJurkat T cells and HUVEC=ND[87]
TRAILRGDCOLO-205 and HT-29 human colon carcinoma++[81]

a Efficacy compared to non-targeted chemotherapeutic agent: Improved efficacy (+), comparable efficacy (=), less efficacy (-), and not determined (ND).

Integrin targeted delivery of nanoparticle-carried chemotherapeutic agents

The third approach is to employ a nanoparticle-carried delivery system. Integrin targeted ligands, such as RGD peptides, can be coated on the surface of nanoparticle carrier system to selectively deliver chemotherapeutic agents to cancer cells or diseased tissues. There are several advantages for the nanoparticle carrier system over conventional drug delivery system. First, multivalent ligands can be conjugated on the surface of the nanoparticle. Multivalency not only significantly improves the binding affinity to the integrin, but also facilitates internalization. Carrier systems, such as liposomes and nanoparticles, after bearing multiple RGD-peptides, can be more readily internalized via receptor-mediated endocytosis. As a result, larger amount of chemotherapeutic agents can be delivered through internalizing receptor-targeted ligand. In addition, high molecular weight of nanoparticle carriers may lead to passive retention in a tumor. Leaky blood vessels and poor lymphatic drainage are commonly presented in tumor. Nanoparticles can penetrate and accumulate in the tumor via those leaky vessels by the enhanced penetration and retention (EPR) effect [89]. In order to protect nanoparticles from protein absorption and prolong the circulation time after administration, polyethylene glycol (PEG) is normally incorporated into the delivery system. The most commonly used nanoparticulate delivery systems include liposomes, polymeric nanosphere, micelles, and polymersomes [90].

The RGD-containing peptide-decorated nanoparticulate delivery system has been extensively investigated. For example, RGD-peptides were coupled to the distal end of poly(ethylene glycol)-coated liposomes (LCL) to obtain a stable long-circulating drug delivery system functioning as a platform for multivalent interaction with integrin αvβ3 [91]. The results showed that RGD-peptide-modified LCL exhibited increased binding to endothelial cells in vitro. Moreover, intravital microscopy demonstrated a specific interaction of these liposomes with tumor vasculature, a characteristic not observed for LCL. In in vivo study, RGD-LCL encapsulating doxorubicin inhibited tumor growth in a doxorubicin-insensitive murine C26 colon carcinoma model, whereas doxorubicin in LCL failed to decelerate tumor growth. Overall, RGD-LCL containing doxorubicin showed superior efficacy over non-targeted LCL in inhibiting C26 doxorubicin-insensitive tumor outgrowth. Likely, these RGD-LCL-doxorubicin antitumor effects are brought about through direct effects on tumor endothelial cells [91]. Recently, cRGDfK peptide was coupled to PEGylated liposome encapsulating anticancer drug (doxorubicin) to target integrin αvβ3-expressing tumor vasculature [92]. The results showed that delivery of targeted nanoparticles inhibited the growth of metastases while eliminating the toxicity and weight loss associated with systemic administration of doxorubicin. The delivery resulted in a 15-fold improvement in tumor and anti-metastatic activity when compared with administration of the free drug. The preferential activity of these nanoparticles on metastases implies that growing metastatic tumors may have a greater dependence on angiogenic vessels and thus could be more susceptible to integrin αvβ3-targeted therapy [93]. In another example, Xiong et al. achieved high tumor accumulation and intercellular delivery of doxorubicin after conjugating synthetic RGD mimic compound with the drug-loading liposome in syngeneic B16 melanoma mouse model. Administration of RGD mimic-modified nanoparticle resulted in retarded tumor growth and prolonged lifespan compared with the non-modified one [94-95]. Similar RGD liposome modification strategies have also been used to deliver other anticancer drugs, such as 5-fluorouracil (5-FU) and paclitaxel, to malignant tumor-bearing animals. The significant anti-primary tumor and antimetastatic activities can also be achieved [96, 52, 97]. The liposomal delivery of a new snake venom disintegrin, contortrostatin (CN) has been reported in an orthotopic human breast tumor xenograft model [98]. This disintegrin modulates its interaction with integrins on tumor cells and angiogenic vascular endothelial cells.

RGD-modified liposomes have also been applied for other disease treatments in addition to cancer therapy. The effect of RGD-modified liposomes on platelet activation and aggregation was investigated in vitro, with and without platelet agonists [99]. RGD-liposomes were found to bind activated platelets at levels significantly greater than the control RGE-liposomes. The RGD-liposomes did not exhibit any statistically significant effect on platelet activation or aggregation. The results demonstrate the ability of the RGD-modified liposomes to target and bind activated platelets without causing significant platelet aggregation and suggests a feasible way for the development of a platelet-targeted anti-thrombogenic drug delivery system. In another study, RGD-coated liposomes were developed for selective cell targeting in cardiovascular drug delivery [100].

Except for using liposome as a carried system, biodegradable polymeric micelles, a type of self-assembled nanoparticle from amphiphilic copolymers, were utilized as a carrier system for integrin targeted doxorubicin delivery [101]. The results showed that attachment of the cyclic RGD ligand greatly enhanced internalization of the micelles in tumor endothelial cells that overexpress integrin αvβ3, apparently through receptor-mediated endocytosis. Biodegradable nanosphere is another attractive carrier system for drug delivery. A RGD-PEG-PLGA nanosphere delivery system was developed for intracellular delivery of doxorubicin to different malignant cancer cells [102]. The results showed this new nanosphere can alleviate the burst drug release effect commonly associated with PLGA nanosphere systems. Recently, self-assembled glycol chitosan nanoparticles were developed to explore whether this construct might function as a prolonged and sustained drug delivery system for RGD peptide [103]. In vitro work demonstrated that RGD-HGC can prolong and sustain release of RGD, lasting for 1 week. RGD-HGC also inhibited HUVEC adhesion to a beta ig-h3 protein-coated surface, indicating an antiangiogenic effect of the RGD peptide in the HGC nanoparticles. In an in vivo study, the antiangiogenic peptide drug formulation of RGD-HGC markedly inhibited bFGF-induced angiogenesis and decreased hemoglobin content in Matrigel plugs. Intratumoral administration of RGD-HGC significantly decreased tumor growth and microvessel density compared to native RGD peptide.

The various conjugates of RGD-peptides and nanoparticle-carried chemotherapeutic agents [52,91-92,94-96,104-105] are summarized in Table 3.

 Table 3 

Integrin targeted delivery of nanoparticle-carried chemotherapeutic agents

NanocarriersTherapeutic agentTargeting motifExperimental modelResultsaRef.
In VitroIn Vivo
LiposomeDoxorubicinc(RGDfK)-PEGC26 murine colon carcinomaND+[91]
LiposomeDoxorubicinRGD10C26 murine colon carcinomaND+[52]
LiposomeDoxorubicinRGD-PEGB16 murine melanoma-+[94,95]
NanoparticleDoxorubicinc(RGDfC)-PEGCl-66 mammary carcinomaNDND[104]
LiposomeDoxorubicinc(RGDfK)-PEGOrthotopic mouse R40P pancreatic carcinoma, human M21L-GFP melanoma, and human SN12C-RFP renal carcinoma++[92]
Liposome5-Fluorouracilc(RGDfK)-PEGB16F10 murine melanoma++[96]
NanoparticleFumagillinIntergrin αvβ3 peptidomimetic antagonistVx-2 rabbit adenocarcinomaND+[105]

a Efficacy compared to non-targeted chemotherapeutic agent: Improved efficacy (+), comparable efficacy (=), less efficacy (-), and not determined (ND).


Development of molecular devices endowed with disease-targeting functions and carrying cytotoxic components enables the specific delivery of chemotherapeutic agents to aberrant tissues, thus increasing their local efficacy while limiting their peripheral toxicity. A large number of studies demonstrated that integrin receptor, in particular integrin αvβ3, is an ideal target of specific delivery of chemotherapeutic agents, because not only integrin αvβ3 can be internalized by cells on activation with anchoring ligands, but also integrin αvβ3 is overexpressed on angiogenetic endothelial cells and absent in pre-existing endothelial cells and normal tissues. Therefore, integrin targeted delivery of chemotherapeutic agents represents a valuable approach and the numerous results obtained to date using different systems open up interesting prospect to future chemotherapy in treatment of various diseases.

Approaches based on multivalent presentation of the RGD ligand will likely continue to receive attention in the future since they provide therapeutic agents with higher binding affinity, prolonged half-life, and passive retention in diseased tissues and are compulsory for cellular uptake. In this regard, the employment of nanoparticle will certainly represents a promising area of continuing investigation. However, the toxicity of novel nanoparticle carriers will have to be clearly addressed in future clinical application. In addition, since integrin αvβ3 expression is not homogeneous all over diseased tissues, strategies that combine multiple biomarkers, such as vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), epidermal growth factor (EGF), and other integrin receptors are expected to generate significantly greater benefits for optimal treatment. Moreover, in order to maximize the therapeutic index, therapeutic multi-modalities, the combination of chemotherapy, radiotherapy, and gene therapy, will be likely more effective than single modality alone. However, the specific synergistic interactions among different modalities have to be investigated and clearly understood. Finally, non-invasive imaging technologies provide precise ways to visualize biological processes of disease at the cellular and molecular level in real time. Thus, imaging-guided therapy should be routinely applied in clinical settings to improve the overall patient outcome and help further steps toward personalized medicine.


The authors would like to acknowledge the Intramural Research Program at the National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH) for financial support.

Conflict of Interest

The authors have declared that no conflict of interest exists.


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Author contact

Corresponding address Corresponding author: Dr. Xiaoyuan Chen, Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), 31 Center Dr, 31/1C22, Bethesda, MD 20892, USA; Tel: 301-451-4246; Email: shawn.chengov; Dr. Kai Chen, Molecular Imaging Center, Department of Radiology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA; Tel: 323-442-3582; Email: chenkaiedu

Published 2011-2-17

Citation styles

Chen, K., Chen, X. (2011). Integrin Targeted Delivery of Chemotherapeutics. Theranostics, 1, 189-200.

Chen, K.; Chen, X. Integrin Targeted Delivery of Chemotherapeutics. Theranostics 2011, 1, 189-200. DOI: 10.7150/thno/v01p0189.

Chen K, Chen X. Integrin Targeted Delivery of Chemotherapeutics. Theranostics 2011; 1:189-200. doi:10.7150/thno/v01p0189.

Chen K, Chen X. 2011. Integrin Targeted Delivery of Chemotherapeutics. Theranostics. 1:189-200.

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