Theranostics 2018; 8(1):124-140. doi:10.7150/thno.19441
Engineered Microenvironments for Maturation of Stem Cell Derived Cardiac Myocytes
1. Department of Biomedical Engineering, University of Miami;
2. Department of Pathology & Laboratory Medicine, University of Miami;
3. DJTMF Biomedical Nanotechnology Institute, University of Miami.
Besser RR, Ishahak M, Mayo V, Carbonero D, Claure I, Agarwal A. Engineered Microenvironments for Maturation of Stem Cell Derived Cardiac Myocytes. Theranostics 2018; 8(1):124-140. doi:10.7150/thno.19441. Available from https://www.thno.org/v08p0124.htm
Through the use of stem cell-derived cardiac myocytes, tissue-engineered human myocardial constructs are poised for modeling normal and diseased physiology of the heart, as well as discovery of novel drugs and therapeutic targets in a human relevant manner. This review highlights the recent bioengineering efforts to recapitulate microenvironmental cues to further the maturation state of newly differentiated cardiac myocytes. These techniques include long-term culture, co-culture, exposure to mechanical stimuli, 3D culture, cell-matrix interactions, and electrical stimulation. Each of these methods has produced various degrees of maturation; however, a standardized measure for cardiomyocyte maturation is not yet widely accepted by the scientific community.
Keywords: Stem cells, Cardiac Myocytes, Differentiation, Maturation, Tissue Engineering.