Theranostics 2022; 12(16):7108-7131. doi:10.7150/thno.74820 This issue Cite


Near-infrared photoimmunotherapy: design and potential applications for cancer treatment and beyond

Danfeng Wei1,2,3*, Jinxin Qi1,2*, Michael R Hamblin4, Xiang Wen1, Xian Jiang1,2 Corresponding address, Hao Yang3,5 Corresponding address

1. Department of Dermatology and National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China.
2. Laboratory of Dermatology, Clinical Institute of Inflammation and Immunology, Frontiers Science Center for Disease-related Molecular Network West China Hospital, Sichuan University, Chengdu 610041, China.
3. NHC Key Lab of Transplant Engineering and Immunology, Organ Transplant Center, West China Hospital, Sichuan University, Chengdu, Chengdu 610041, China.
4. Laser Research Centre, Faculty of Health Science, University of Johannesburg, Doornfontein 2028, South Africa.
5. Sichuan Provincial Engineering Laboratory of Pathology in Clinical Application, West China Hospital, Sichuan University.
* These authors contributed equally to this work.

Wei D, Qi J, Hamblin MR, Wen X, Jiang X, Yang H. Near-infrared photoimmunotherapy: design and potential applications for cancer treatment and beyond. Theranostics 2022; 12(16):7108-7131. doi:10.7150/thno.74820.
Other styles

File import instruction


Graphic abstract

Near-infrared photoimmunotherapy (NIR-PIT) is a newly developed cancer treatment modality based on a target-specific photosensitizer conjugate (TSPC) composed of an NIR phthalocyanine photosensitizer and an antigen-specific recognition system. NIR-PIT has predominantly been used for targeted therapy of tumors via local irradiation with NIR light, following binding of TSPC to antigen-expressing cells. Physical stress-induced membrane damage is thought to be a major mechanism underlying NIR-PIT-triggered photokilling. Notably, NIR-PIT can rapidly induce immunogenic cell death and activate the adaptive immune response, thereby enabling its combination with immune checkpoint inhibitors. Furthermore, NIR-PIT-triggered “super-enhanced permeability and retention” effects can enhance drug delivery into tumors. Supported by its potential efficacy and safety, NIR-PIT is a rapidly developing therapeutic option for various cancers. Hence, this review seeks to provide an update on the (i) broad range of target molecules suitable for NIR-PIT, (ii) various types of receptor-selective ligands for designing the TSPC “magic bullet,” (iii) NIR light parameters, and (iv) strategies for enhancing the efficacy of NIR-PIT. Moreover, we review the potential application of NIR-PIT, including the specific design and efficacy in 19 different cancer types, and its clinical studies. Finally, we summarize possible NIR-PIT applications in noncancerous conditions, including infection, pain, itching, metabolic disease, autoimmune disease, and tissue engineering.

Keywords: near-infrared photoimmunotherapy, IRdye 700DX, target-specific photosensitizer conjugate, cancer-treatment, noncancerous diseases


Owing to its high morbidity and mortality, cancer remains a great threat to human health despite the enormous amount of associated research. Photodynamic therapy (PDT) is a promising treatment modality as it offers efficient antitumor destruction with limited adverse effects via localized activation of a photosensitizer (PS) by light irradiation. PDT depletes cancer cells through reactive oxygen species generation or physical stress, consequently inducing cellular apoptosis or necrosis [1]. Although PDT has shown many promising results, its clinical application is limited by poor PS solubility and lack of targeted delivery methods for most PSs.

A newly developed molecular-targeted antitumor phototherapy approach, called near-infrared photoimmunotherapy (NIR-PIT), has shown great potential in overcoming the challenges associated with PDT. NIR-PIT employs a tumor-specific binding ligand, such as a monoclonal antibody (mAb), mAb fragment, or small-molecule ligand that is conjugated to IRdye 700DX (IR700)—a silicon phthalocyanine dye—which is activated by NIR light to cause immunogenic cell death (ICD) [2, 3]. After in vivo administration, the target-specific photosensitizer conjugate (TSPC) distributes into the tumor and binds to the antigen-expressing cell membranes (Figure 1A-B). Following topical NIR light irradiation at the tumor site, photoinduced dissociation of hydrophilic axial ligands from IR700 leads to the aggregation of hydrophobic complexes on the cell membrane, which causes transmembrane damage and reduces cell membrane integrity (Figure 1C-D). The tumor cells then swell and burst, releasing intracellular contents due to the influx of water. As a result, NIR-PIT induces TSPC-bound tumor cell necrosis [4-6]. The rapid necrotic death of cancer cells results in release of damage-associated molecular patterns (DAMPs), including calreticulin, heat shock proteins (HSPs), ATP, HMGB1, and other antigens, which altogether activate dendritic cells (DCs) to stimulate the infiltration of CD8+ cytotoxic T lymphocytes (CTLs). The activated T cells then elicit a host immune response against residual tumor cells following direct killing by NIR-PIT. This is the process of NIR-PIT-induced ICD [3, 7-9]. In contrast, the normal bystander cells expressing minimal, or no, target antigens are spared. The remarkable cytotoxicity and specificity for tumors make NIR-PIT an attractive therapeutic modality that differs from conventional PDT. In fact, NIR-PIT-triggered ICD has the potential to augment the efficacy of various cancer immunotherapies, which will be discussed later in a subsection.

PS is a critical component of the “magic bullet” TSPC for NIR-triggered cytotoxicity. Among several PSs that have been investigated, the new-generation dye IR700 (Figure 1B) has many features that make it suitable for cancer treatment. First, the chemical modification-yielding hydrophilic feature makes IR700 superior for TSPC preparation and application compared to most conventional PSs that are typically hydrophobic. Moreover, its water-soluble property prevents IR700 aggregation or precipitation during the preparation of TSPC in buffered physiological solution, even at high dye-to-protein ratios. Upon intravenous injection, hydrophilic IR700 does not affect the tumor-homing ability or tissue distribution of antibodies, whereas hydrophobic PS-antibody conjugates may aggregate and accumulate in the liver where they are metabolized [2, 6, 7]. In addition, the hydrophilic property of IR700 prevents interactions with non-antigen-expressing cell membranes, leading to little nonspecific cytotoxicity. Second, NIR light irradiation can cleave the axial ligands—consisting of three sulfonic acids as water-soluble protecting groups—of the IR700 structure, resulting in TSPC-bound membrane destruction and cell necrosis [4, 6]. Thus, it is not necessary for IR700-based TSPCs to be internalized by cells, nor do they necessarily rely on reactive oxygen species for their anticancer activity [10]. In contrast, conventional PSs, which are generally hydrophobic, must be internalized by targeted cells, thus, hindering their target selectivity and antitumor efficacy [1, 11]. Third, IR700 molecules can be modified with an N-hydroxysuccinimide ester (NHS) group, which facilitates conjugation with antibodies or other tumor-homing species. Fourth, IR700 is activated by NIR light (690 nm), which allows for relatively deep tissue penetration. Conventional PSs typically absorb visible light with less penetration into deep tissues [2, 12, 13]. Fifth, similar to other phthalocyanine PSs, IR700 has a large molar extinction coefficient, moderate quantum yield, and relatively good photochemical stability under natural light [2, 14]. Sixth, IR700 can emit near-infrared fluorescence, enabling the fluorescent imaging of targeted tissues, which may facilitate more specific tumor irradiation. Moreover, a commercially-available indocyanine green-camera can monitor the therapeutic progress in real-time with decay of the IR700 fluorescence signal even during therapeutic exposure to NIR light [15, 16].

An effect called “super-enhanced permeability and retention” (SUPR) was observed after NIR-PIT. As NIR-PIT rapidly kills perivascular cancer cells, tumor vascular permeability greatly increases, leading to much higher (up to 24-fold) nanoparticle accumulation within the treated tumor [17]. Hence, nanoparticle-based drugs can show an improved antitumor effect with the help of NIR-PIT [18]. SUPR effects also facilitate the delivery of TSPC itself more deeply into the tumor after the initial NIR-PIT; repeated NIR exposures result in a better therapeutic response (Figure 2) [19-21]. In theory, repeated NIR-PIT should have no safety concerns [3, 4, 19]. That is, NIR light has almost no limitations on the cumulative irradiation dosage, as it is nonionizing and harmless to DNA or normal cells. IR700, whether intact or catabolized, is readily excreted into the urine without any long-term photosensitizing effects [2].

The ability of NIR-PIT to induce SUPR effects and trigger anticancer host immune responses has previously been reviewed. Its application in the local control of solid cancers, as well as certain molecular targets, antitumor mechanisms, and possible challenges in its implementation, have recently been discussed [3, 8, 22-25]. Here, in the first section, we provide an update on the broad spectrum of target molecules used for NIR-PIT, various types of receptor-selective ligands for the design of a TSPC “magic bullet” (Tables 1-4), NIR light parameters, and potential strategies for enhancing the antitumor effects of NIR-PIT. In the second section, we provide a comprehensive overview of NIR-PIT applications for treating 19 different types of cancers, arising from different human tissues and organs. Meanwhile, we summarize the clinical trials on NIR-PIT for tumor treatment. In the third section, we prospectively review the possible applications of NIR-PIT in non-malignant diseases, such as infection, pain, itching, metabolic diseases, autoimmune diseases, and tissue engineering, as well as the limitations and challenges encountered when employing NIR-PIT.

Selection of therapeutic targets

The expression level of therapeutic targets in tumors

The accurate selection of molecules to be targeted by ligands is critical for cancer therapy. For most cancer-targeted therapies, significantly higher expression of the target molecules on cancer cells relative to that on normal cells is crucial for reducing unwanted off-target effects. However, in the case of NIR-PIT, researchers have primarily considered the distribution and function of the targeted antigens as NIR irradiation can be spatially confined to the tumor, and normal tissue is generally not damaged when using TSPC-delivered PS. Therefore, NIR-PIT has a wider target pool than other methods using other payloads that do not require external activation.

 Figure 1 

Scheme of NIR-PIT based on TSPCs. (A) The systematically injected TSPCs can be locally activated following NIR irradiation at lesion sites. (B) Schematic illustration of TSPC. (C) TSPCs bind to specific target molecules on the cell surface followed by NIR irradiation. (D) Photoinduced dissociation of hydrophilic axial ligands from IR700 causes transmembrane damage through the remaining hydrophobic complexes on the cell membrane. NIR-PIT: near-infrared photoimmunotherapy; TSPC: target-specific photosensitizer conjugate.

Theranostics Image
 Figure 2 

SUPR effects induced by NIR-PIT. (A) Following injection, TSPCs are delivered through the vasculature and then bind to the tumor sites. (B) Tumor necrosis following NIR irradiation. (C) Super enhanced delivery of TSPCs into the treated tumor bed after the first NIR-PIT. (D) Repeated NIR irradiation enhances the therapeutic effect of NIR-PIT. SUPR: super enhanced permeability and retention.

Theranostics Image
 Figure 3 

Binding site barrier of TSPC in a tumor. The preferential accumulation of high-affinity ligands/antibodies in the immediate proximity of blood vessels might hinder the deep penetration of TSPCs into the tumor parenchyma.

Theranostics Image

Although the expression level of target molecules typically does not cause safety concerns in NIR-PIT, it does impact the therapeutic effect. That is, a higher expression level of the target will induce a more potent antitumor effect as more TSPCs can bind to the target and accumulate in the tumor site. However, using a high-affinity ligand/antibody recognizing a highly-expressed target molecule may not always prove beneficial. Deep penetration into the tumor parenchyma may be hampered by accumulation of high-affinity binding ligands/antibodies in the immediate proximity of blood vessels, a phenomenon known as the “binding site barrier” (Figure 3) [26]. Therefore, a more homogeneous distribution of TSPCs in the tumor parenchyma could be achieved by using an additional antibody with low antigen affinity, or an antibody that recognizes a low-expression antigen. Nakajima et al. developed a TSPC by employing the anti-epidermal growth factor receptor (EGFR) antibody panitumumab (Pan) plus the anti-CD25 antibody basiliximab (Bas) for targeted delivery of IR700 to the ATAC4 cell line, with high expression of EGFR and low expression of CD25. No significant difference was observed in the therapeutic response to NIR-PIT using Pan-IR700 or the Pan-IR700/Bas-IR700 mixture in vitro. However, the cocktail of Pan-IR700 + Bas-IR700 resulted in greater inhibition of tumor growth and longer survival in tumor-bearing mice than either Pan-IR700 or Bas-IR700 alone. Analysis of the TSPC microdistribution in the tumors showed that Pan-IR700 was more peripherally localized despite the high expression of EGFR on tumor cells, whereas Bas-IR700, targeting the low-expression CD25, was distributed uniformly throughout the tumor, including the center where Pan-IR700 was not found [27].

Therapeutic targets on cancer cells

Despite a wide range of options, due to their overexpression and wide distribution in multiple cancers, EGFR and human epidermal growth factor receptor type 2 (HER2) are currently the most frequently used targets in NIR-PIT [28-32]. The ability of NIR-PITs to eliminate EGFR- or HER2-expressing cancer cells have been used to treat multiple types of tumors, including brain [33], head and neck [34, 35], bladder [36-38], lung [39-42], ovarian [43-45], breast [46-50], esophageal [51], and gastrointestinal [52-56] cancers. Tumor-associated antigens (TAAs), such as carcinoembryonic antigen (CEA) for gastric, pancreatic, and colorectal [12, 57, 58] cancers, glypican-3 (GPC3) for hepatocellular carcinoma [59, 60], prostate-specific membrane antigen (PSMA) for prostate cancer [61-64], CD20 for B-cell lymphoma [65, 66] and CD146 for melanoma [67], are also attractive targets for NIR-PIT. Indeed, the efficacy of targeting some of these antigens has been assessed clinically for tumor imaging and treatment. Drug-resistance-related proteins are another important type of antigen that can be used for NIR-PIT. For example, P-glycoprotein (Pgp) mediates multidrug resistance in numerous cancers by pumping anticancer drugs out of cancer cells [68]. As such, Pgp is an attractive target for NIR-PIT due to its critical function and selective expression by cancer cells. In xenograft models of chemoresistant cancer, anti-Pgp antibodies and anti-Pgp Fab-mediated NIR-PIT elicit comparable spatially selective tumor ablation, and prolong the survival of treated mice [69, 70]. By contrast, Fab conjugates require a shorter time to obtain peak accumulation and are distributed more homogeneously in tumors, which is beneficial for clinical translation [70]. When combined with liposomal doxorubicin (Doxil), an FDA-approved cancer nanodrug, Pgp-targeted NIR-PIT improves the therapeutic effect of Doxil by depleting multidrug resistant (MDR) cancer cells and increasing the tumor penetration of Doxil [71]. Surface markers of cancer stem cells (CSCs), such as CD44 [72, 73] or CD133 [74], have been used in NIR-PIT to kill aggressive subpopulations of cells in the tumor, resulting in good therapeutic effects. The expression of immune evasion-related CD47 on tumor cells can serve as a “don't eat me” signal after binding to its receptor SIRPα expressed on phagocytes. Interestingly, anti-CD47 antibody-directed NIR-PIT was found to not only cause phototoxicity but also induce phagocytosis by blocking the CD47-SIRPa interaction [75].

 Table 1 

Therapeutic targets on cancer cells.

Target moleculeIR700 carrierTSPCCancerCombination treatmentTumor modelRef.
AC133/CD133AntibodyAC133-IR700GlioblastomaIn vitro and in vivo; ORT[74]
ꞵ-D-galactose receptorLigandGSA-IR700Peritoneal disseminated ovarian cancerIn vitro and in vivo; ORT[163]
Cadherin-17AntibodyARB102-IR700Pancreatic cancerIn vitro and in vivo; s.c.[171]
CB2RLigandmbc94-IR700Brain cancerIn vitro and in vivo; s.c.[118, 119]
CD146AntibodyYY146-IR700MelanomaIn vitro and in vivo; s.c.[67]
CD20AntibodyRituximab-IR700B-cell lymphomaIn vitro and in vivo; s.c.[65]
CD20AntibodyNuB2-IR700B-cell lymphomaIn vitro and in vivo; s.c.[66]
CD29AntibodyAnti-CD29-IR700MelanomaCTLA4 blockadeIn vitro and in vivo; s.c.[159]
CD276Fab fragment of antibodyαCD276/Fab-IR700Breast cancerαPD-L1 antibodyIn vitro and in vivo; s.c. and lung metastasis[90]
CD44AntibodyAnti-CD44-IR700Triple-negative breast cancerIn vitro and in vivo; ORT[72]
CD44AntibodyAnti-CD44-IR700Oral squamous cell carcinomaIn vitro and in vivo; s.c.[73]
CD44AntibodyAnti-CD44-IR700Colon cancer, lung carcinoma, oral carcinomaPD-1 blockadeIn vitro and in vivo; s.c.[102]
CD44AntibodyAnti-CD44-mAb-IR700Colon cancer, lung carcinoma, oral carcinomaCTLA4 blockadeIn vitro and in vivo; s.c.[103]
CD44Antibodyanti-CD44-mAb-IR700Colon cancer, lung carcinoma, oral carcinomaIL-15In vitro and in vivo; s.c.[104]
CD47AntibodyB6H12-IR700Bladder cancerIn vitro and in vivo; s.c.[75]
CEAAntibody45IR (C2-45 IR700)Gastric adenocarcin-omaIn vitro and in vivo; s.c.[12]
CEAAntibodyAnti-CEA-IR700pancreatic cancerIn vitro and in vivo; ORT[169]
CEAAntibodyAnti-CEA-IR700After bright light surgery in mouse models of pancreatic cancerIn vitro and in vivo; ORT[57]
CEAAntibodyAnti-CEA-IR700After bright light surgery in PDOX model of pancreatic cancerIn vitro and in vivo; ORT[170]
CEAAntibodyDTPA-hMN-14-IR700Colorectal cancerIn vitro and in vivo; s.c.[136]
CEAAntibodyM5A-700Colorectal cancerIn vitro and in vivo; ORT[58]
c-KITAntibody12A8-IR700Gastrointest-inal stromal tumorsIn vitro and in vivo; ORT[148]
CLAAntibodyCLA-IR700DXMycosis fungoidesIn vitro[161]
DLL3AntibodyRova-IR700Lung cancerIn vitro and in vivo; s.c.[151]
EGFRAntibodyCet-IR700Head and neck cancerIn vivo/clinical trial[109, 110]
EGFRAntibodyPan-IR700Head and neck cancerIn vitro and in vivo; s.c. (subtotal tumor resection model)[34]
EGFRAntibodyPan-IR700Bladder cancerIn vitro and in vivo; s.c.[36]
EGFRAntibodyCan225-IR700Bladder cancerIn vitro and in vivo; s.c.[38]
EGFRAntibodyCet-IR700 or pan-IR700Breast cancer, cutaneous squamous cell carcinomaIn vitro and in vivo; ORT[97]
EGFRAntibodyCet-IR700Triple-negative breast cancerIn vitro and in vivo; s.c.[46]
EGFRAntibodyPan-IR700Breast cancerIn vitro and in vivo; ORT[50, 100]
EGFRAntibodyPan-IR700Breast cancerCyEt-Pan-DuoIn vitro and in vivo; s.c.[47]
EGFRAntibodyPan-IR700Lung cancerIn vitro and in vivo; ORT[40]
EGFRNanobodyNanobody-IR700 (7D12, 7D12-9G8)Head and neck cancerIn vitro and in vivo; ORT[35]
EGFRAffibodyZEGFR:03115-IR700GlioblastomaIn vitro and in vivo; s.c.[33]
EGFRAffibodyZEGFR:1907-IR700Multidrug resistant colorectal cancerIn vitro and in vivo; s.c.[141]
GPC1AntibodyMiltuximab®-IR700Bladder, prostate, brain,ovarian cancer cell linesIn vitro[130]
GPC3Antibody and heavy-chain antibodyHN3-IR700, YP7-IR700Hepatocellu-lar carcinomaIn vitro and in vivo; s.c.[59]
GPC3AntibodyYP7-IR700Hepatocellu-lar carcinomaNanosized albumin-bound paclitaxelIn vitro and in vivo; s.c.[60]
GPA33ScFvA33scFv-R700Colorectal tumorsIn vitro and in vivo; s.c.[138]
HER2AntibodyTra-IR700Lung metastasesIn vitro and in vivo; ORT and lung metastasis[39, 41]
HER2AntibodyTra-IR700Pleural disseminated non-small cell lung carcinomaIn vitro and in vivo; ORT[42]
HER2AntibodyTra-IR700Disseminated peritoneal ovarian cancerIn vitro and in vivo; ORT[43]
HER2AntibodyTra-IR700 and
Gastric cancerIn vitro and in vivo; s.c.[53]
HER2AntibodyTra-IR700Peritoneal dissemination of gastric cancerIn vitro and in vivo; ORT[54, 55]
HER2AntibodyTra-IR700Gastric cancer5-FUIn vitro and in vivo; s.c.[52]
HER2Nanobody1D5-IR700, 1D5-18A12-IR700Breast cancerIn vitro and in vivo; ORT[88]
HER2AffibodyHER2 Affibody-IR700Breast CancerIn vitro[124]
HER2AffibodyZHER2:2395-IR700Ovarian cancerIn vitro and in vivo; s.c.[93]
HGFRNanobodyG2c-PSHepatocellu-lar, gastric cancer cell linesIn vitro[91]
HSPGVLPAU-011 (IR700)Primary uveal melanomaIn vitro and in vivo; s.c. (murine) and ORT (rabbit)[95]
MesothelinAntibodyHYP218-IR700Cutaneous squamous cell carcinoma stably expressing human mesothelinIn vitro and in vivo; s.c.[142]
PSMAAntibody, Diabody, MinibodyPSMA-Db-IR700, PSMA-Mb-IR700, PSMA-IgG-IR700Prostate cancerIn vitro and in vivo; s.c.[89]
PSMAAntibody111 In-DTPA-D2B-IR700Prostate cancerIn vitro and in vivo; s.c.[63]
PSMAAntibodyAnti-PSMA- IR700Prostate cancerIn vitro and in vivo; s.c.[62]
PSMAPeptidePSMA-1-Pc413, PSMA-1-IR700Prostate cancerIn vitro and in vivo; s.c.[64]
PSMASmall moleculeYC-9Prostate cancerIn vitro and in vivo; s.c.[61]
PD-1AntibodyAvelumab-IR700Papillary adenocarci-noma of lungIn vitro and in vivo; s.c.[154]
PDPNAntibodyNZ-1-IR700Malignant pleural mesotheliomaIn vitro and in vivo; s.c. and disseminated pleural models[155]
PgpAntibodyPab-IR700Multidrug-resistant cervical cancerIn vitro and in vivo; s.c.[69]
PgpAntibody, Fab fragmentFab-IR700, Pab-IR700Multidrug-resistant cervical cancerIn vitro and in vivo; s.c.[70]
TROP2AntibodyTROP2-IR700Biliary & pancreatic cancerIn vitro and in vivo; s.c.[134]
US28NanobodyVUN100-IR700GlioblastomaIn vitro (2D and 3D cultures)[92]

CEA: carcinoembryonic antigen; GSA: galactosyl serum albumin; HGFR: hepatocyte growth factor receptor, c-Met; PDOX: patient-derived orthotopic xenograft; TSPC: target-specific photosensitizer conjugate; VLP: virus-like particle; s.c, subcutaneous; ORT, orthotopic

 Table 2 

Therapeutic targets on non-tumor cells in the tumor microenvironment (TME).

Target moleculeIR700 carrierTSPCCancerCombination treatmentTumor modelRef.
CD25AntibodyAnti-CD25-IgG-IR700, Anti-CD25-F(ab′)2-IR700Colon cancerIn vitro and in vivo; s.c.[82]
CD25F(ab')2 fragmentsAnti-CD25-F(ab')2-IR700Colon cancer, lung carcinoma, Prostate cancerIn vitro and in vivo; s.c.[84]
CD73AntibodyαCD73-IR700Pancreatic ductal adenocarcinoma(subcutaneous), breast cancer (orthotopic)PD-1 blockadeIn vitro and in vivo; s.c. and ORT[77]
FAPAntibodyFAP-IR700Esophageal squamous cell carcinomaIn vitro and in vivo; s.c.[76]
FAPAntibodyFAP-IR700Esophageal cancer5-FUIn vitro and in vivo; s.c.[146]
Integrin αvβ3NanobodycRGD-PEG-HSA-IR700Ovarian tumor spheroidsIn vitro (in 3D cultures)[79]
Integrin αvβ3NanobodyIR700-PEG-PGlu-cRGDxGlioblastomaIn vitro and in vivo; s.c.[80]
Integrin αvβ3NanobodyRGD-8PEG-IR700Melanoma spheroidsIn vitro (in 3D cultures)[81]
PDGFRβAffibodyDimeric Z-PDGFRβ-IR700Colorectal cancerIn vitro and in vivo; s.c.[78]
VEGFR-2AntibodyDC101-IR700Gastric cancerIn vitro and in vivo; s.c.[56]
VISTAAntibodyanti-VISTA-IR700Colon cancer, lung carcinomaIn vitro and in vivo; s.c.[85]

s.c, subcutaneous; ORT, orthotopic

 Table 3 

Combined targeting of multiple targets or epitopes.

Target moleculeIR700 carrierTSPCCancerCombination treatmentTumor modelRef.
EGFR, HER2AntibodyPan-IR700,
Cholangiocarci-nomaIn vitro and in vivo; s.c.[135]
EGFR, HER2AntibodyPan-IR700,
Bladder cancerIn vitro and in vivo; s.c.[86]
EGFR, HER2AntibodyCet-IR700,
Esophageal adenocarcinomaTKIIn vitro[51]
EGFR, HER2AntibodyCet-IR700,
Cutaneous squamous cell carcinomaIn vitro and in vivo; s.c.[20]
EGFR, CD25AntibodyPan-IR700,
Cutaneous squamous cell carcinomaIn vitro and in vivo; s.c.[27]
EGFR, EpCAM and CSPG4ScFvScFv-425 (EGFR), EpCAM(scFv), CSPG4(scFv), -SNAP-IR700Triple-negative breast cancer cell linesIn vitro[49]
CD44, CD25AntibodyAnti-CD44-IR700, anti-CD25-IR700Colon cancer, lung carcinoma, oral carcinomaIn vitro and in vivo; s.c.[83]

TKI: tyrosine kinase inhibitor; s.c, subcutaneous

 Table 4 

NIR-PIT for non-malignant diseases.

Target moleculeIR700 carrierTSPCNoncancerous diseasesModelRef.
FAPAntibody28H1-700DXArthritisIn vitro and in vivo collagen-induced arthritis mouse model[188]
FAPAntibody28H1-IR700Systemic sclerosisIn vitro (2D and 3D cultures)[191]
GLP-1RLigand, peptideExendin-4-IR700Hyperinsulinemic hypoglycemiaIn vitro and in vivo; s.c.[185]
Gp41Antibody7B2-IR700HIVIn vitro[176]
IgYAntibodyCA-IgY-IR700Candida albicans infectionIn vitro and in vivo
CA-infected cutaneous ulcers mouse model
IL-31LigandIL-31K138A-SNAP-IR700ItchIn vitro and in vivo mouse models of atopic dermatitis, of cutaneous amyloidosis, and of psoriasis[182]
IsaAAntibody1D9-IR700Staphylococcus aureus infectionsIn vitro and in vivo Galleria mellonella larval infection model, human postmortem orthopedic implant infection model[173]
TrkA/ P75LigandNGFR121W-SNAP-IR700Acute, inflammatory, neuropathic, and joint painIn vitro and in vivo mouse models of pain[179]
TrkBLigandBDNFSNAP-IR700Mechanical pain hypersensitivityIn vitro and in vivo mouse pain models[177]

s.c, subcutaneous

Therapeutic targets on non-tumor cells in the tumor microenvironment (TME)

NIR-PIT can be designed to damage components of the TME, including vascular cells, cancer-associated fibroblasts (CAFs), and tumor-infiltrating immune cells, such as regulatory T cells (Tregs) and unwanted phagocytes [75-77]. NIR-PIT targeting tumor blood vessels can mediate tumor cell death by blocking the supply of nutrients and oxygen [56, 78-81]. However, severe damage to tumor blood vessels induced by NIR-PIT might also hinder further drug delivery into the tumor parenchyma. Nevertheless, whether NIR-PIT-induced neovasculature damage inhibits immune activation, and the SUPR effects, remain to be investigated.

Unlike tumor/vasculature-specific antibody-mediated NIR-PIT, CD25-targeted NIR-PIT has been used to selectively eradicate tumor-infiltrating Tregs—a type of immunosuppressive cell with high CD25 expression—to treat a broad range of cancers in murine models, including lung, colon, and prostate cancers [82-84]. The antitumor effect of CD25-targeted NIR-PIT involves activation of effector CD8+ T cells and NK cells, which are present in the TME but suppressed by Tregs. Hence, by selective depletion of Tregs, CD25-targeted NIR-PIT not only induces regression of irradiated tumors, but also elicits a therapeutic response against distant non-treated tumors in a tumor-antigen-specific manner. Additionally, repeated applications of NIR-PIT are feasible, which may not be the case for ionizing radiation. Despite CD25 upregulation in activated CD8+ T and NK cells, CD25 expression on these effector cells is downregulated after repeated NIR-PIT following a 4-day interval [84]. However, it is worth noting that CD25-targeted NIR-PIT could be less effective in cancers where Tregs do not play a major role. Meanwhile, CD73 (extracellular-5′-nucleotidase) is overexpressed in many cancer cells as well as TME-related cells, such as Tregs, myeloid-derived suppressor cells (MDSCs), and tumor-associated macrophages, however, is absent in effector CD8+ T cells, NK cells and DCs. Thus, by locally depleting multiple immunosuppressor cell types and significantly increasing CD8+ effector T cells in tumors, the CD73-targeted NIR-PIT + anti-PD1 antibody combination treatment could overcome acquired resistance to PD-1 blockade, and eradicate advanced tumor xenografts in mice, irrespective of CD73 expression levels in tumor cells [77]. Another immunotherapy-related target molecule is V-domain immunoglobulin suppressor of T cell activation (VISTA), which is an immune checkpoint molecule of the B7 ligand family. VISTA is broadly expressed on Tregs and MDSCs of tumor-infiltrating lymphocytes, as well as various primary human tumors. VISTA-targeted NIR-PIT inhibits tumor growth and prolongs survival in murine tumor models by activating CTLs and DCs in the TME. Taken together, by editing the tumor microenvironment, NIR-PIT can dramatically activate systemic antitumor immunity against both the treated and the untreated distant tumors [85].

Combined targeting of multiple targets or epitopes

TSPCs recognizing different antigens can be combined in NIR-PIT treatment to enhance the therapeutic effects [49, 53, 86]. Due to tumor heterogeneity, NIR-PIT against a single target may not eradicate all tumor cells and may not consistently achieve therapeutic effects in different patients. Thus, NIR-PIT against multiple molecular targets, such as the combination of EGFR with HER2 for bladder cancer therapy [86], as well as a cocktail of EGFR, epithelial cell adhesion molecule (EpCAM), and chondroitin sulfate proteoglycan 4 (CSPG4) for the treatment of triple-negative breast cancer (TNBC) [49], exhibit therapeutic effects superior to NIR-PIT against a single target. Moreover, antigens on different cell populations in the TME can be targeted simultaneously in NIR-PIT to improve therapeutic efficacy. NIR-PIT exploiting anti-CD44 and anti-CD25 antibodies significantly inhibits tumor growth and prolongs survival in several tumor animal models compared with NIR-PIT based on a single target, owing to the depletion of both cancer cells and immunosuppressive cells in the TME [83]. A combination of nanobody-mediated NIR-PIT, which targets vascular endothelial growth factor receptor 2 (VEGFR2) on tumor endothelial cells, and EGFR on tumor cells, has also shown improved efficacy in a coculture model of endothelial and cancer cells [87]. In addition to targeting different antigens, NIR-PIT can act on different epitopes of the same antigen. For instance, the HER2-targeted antibodies trastuzumab and pertuzumab increased the specific delivery of IR700 to HER2-positive tumor cells with no competition for antigen binding, thus leading to a stronger anti-tumor response after NIR exposure [53]. Similarly, a bispecific nanobody designed by engineering monovalent nanobodies into a biparatopic construct could recognize two non-overlapping epitopes on the HER2 molecule. NIR-PIT using this nanobody not only enhances therapeutic effects compared with NIR-PIT targeting individual epitopes, but also eradicates trastuzumab-resistant HER2-positive breast cancer, providing a promising new approach for future clinical practice [88]. Therefore, it is likely that multiple TSPCs targeting different antigens or epitopes will achieve an augmented antitumor effect.

Construction of target-specific PSs

TSPCs with different sizes, affinities, and pharmacokinetics can influence the therapeutic effects of NIR-PIT. An intact antibody has a relatively large size, and thus exhibits poor penetration in solid tumors, limiting the therapeutic efficacy. In this regard, genetically engineered small antibody fragments may retain a high affinity for the antigen while improving the intratumoral delivery of TSPCs. Moreover, the smaller size can enable rapid accumulation of TSPC in the tumor along with rapid systemic clearance. Generation of TSPCs comprising with a diabody (Db), minibody (Mb) [89], Fab [70, 82, 90], nanobody [35, 91, 92], affibody [78, 93], peptide [79, 80], small molecule [63, 94], or recombinant virus-like particles (VLPs) [95], exhibit antitumor effects similar to, or better than, those shown by NIR-PIT with a full-size IgG antibody. However, TSPCs based on whole antibodies are also likely to exhibit a higher affinity for antigens and superior tumor retention compared with their fragments, and could be more suitable for repeated light exposure to optimize therapeutic efficacy without increasing side effects [62].

Shirasu et al. reported an interesting universal TSPC construction, expanding the applicability of NIR-PIT. They conjugated IR700 with NeutrAvidin, which specifically binds to a range of biotinylated tumor-homing molecules. This TSPC indirectly binds to targeted cells with the help of anti-CEA or anti-EpCAM biotinylated antibodies and exhibits cytotoxicity levels, after light irradiation, similar to those elicited by other NIR-PITs, which TSPCs bind directly to the cell membrane [96].

As discussed previously, the affinity of TSPC can affect the antitumor response of NIR-PIT due to the “binding site barrier” [26]. Hence, when constructing TSPCs, it is important to consider the balance between the affinity and tumor penetration to enhance therapeutic efficacy. For example, in a study on nanobody-based NIR-PIT, the researchers altered the affinity of their TSPCs by varying the number of arginine-glycine-aspartic acid (RGD) peptides that could specifically bind to integrins on the cell membrane. They revealed that a polymer containing cyclic 15-tandem repeats of RGD peptides in one construct permitted preferential accumulation in the tumor without compromising penetration to deeper areas, when compared with 5-repeat RGD peptides [80].

The pharmacokinetics of TSPC also affect NIR-PIT efficacy. A previous study compared TSPCs constructed using different anti-EGFR antibodies. Those TSPCs with two antibodies, namely, cetuximab (cet) and panitumumab (Pan), exhibited equal affinity and identical phototoxicity in vitro. However, Pan-IR700 exhibited an antitumor response superior to that of cet-IR700 in vivo. Examination of the biodistribution revealed that cet-IR700 had lower tumor accumulation and more rapid hepatic catabolism than Pan-IR700 [97]. Hence, they concluded that both agents could be useful in certain situations. For instance, the shorter half-life of cet-IR700 might lead to fewer safety concerns, while the longer retention of Pan-IR700 in the circulation could achieve better penetration into the tumor when repeated NIR-PIT is applied.

Notably, when TSPCs specifically bind to their targeted receptors, they may transiently block receptor function, thereby, altering therapeutic efficacy. In a study that eradicated Tregs using anti-CD25-targeted NIR-PIT, an IgG-directed TSPC was reported as less effective than an F(ab')2-guided TSPC. F(ab')2-mediated NIR-PIT recruits a higher number of infiltrating lymphocytes to the tumor. The slower clearance of anti-CD25-IgG-IR700 might account for its lower efficacy, as the sustained circulation of anti-CD25-IgG-IR700 can impair the binding of activated effector T cells to IL-2, a cytokine that promotes T cell proliferation [82]. Other factors, such as solvent pH and conjugation ratio, can also impact the stability and efficacy of TSPCs [98].

 Figure 4 

An overview of NIR-PIT for treating 19 different types of cancers, including the clinical applications or ongoing clinical trials for treatment of head and neck squamous cell carcinoma, melanoma, and cutaneous squamous cell carcinoma.

Theranostics Image

NIR light irradiation

NIR light is an important component of NIR-PIT. While the light dose in NIR-PIT is typically between 50 and 150 J/cm2 [18, 50, 99], increasing the light dose leads to more potent cytotoxicity. However, the power density (mW/cm2) of the light source is not as critical to the effectiveness of NIR-PIT as the total light dose (J/cm2) [100]. When comparing light sources, LEDs have proven less effective than lasers [101].

Researchers are currently seeking improved strategies to deliver NIR light. In addition to delivering NIR irradiation from outside the body, endoscopic delivery is also being explored [54, 55]. In particular, this approach could achieve better efficacy against tumor growth and its dissemination to the lungs [39] or body cavities, such as the bladder, abdominal cavity, or pleural cavity. Meanwhile, applying NIR-PIT during surgery allows for direct exposure of the tumor bed to NIR light as well as removal of any residual tumor. Implantable wireless-powered LEDs have also been developed to treat tumors in deep tissues. However, to address safety concerns, the power supply is separated from the light system. In fact, a newly developed wireless light system can emit NIR light up to 20 cm away from the power supply, thus covering most lesions in the human body. Moreover, this LED system can irradiate tumors over an extended period of time [99].

Combinational strategies for enhancing the antitumor effect of NIR-PIT

A commonly used strategy to improve the antitumor effect is combining NIR-PIT with other therapeutic agents, such as nanodrugs [80], chemotherapeutic drugs [52], NIR light-responsive drugs (e.g., CyEt-panitumab-duocarmycin) [47], and immune checkpoint inhibitors [90, 102]. The SUPR effects or the synergistic antitumor mechanisms may enhance the antitumor effects of these combinations. CD276 is a transmembrane glycoprotein overexpressed in both tumor cells and the tumor vasculature. Interestingly, CD276-mediated NIR-PIT increases the activation and maturation of DCs, and markedly increases the level of programmed cell death protein 1 (PD-L1) in tumors. When combined with αPD-L1 treatment, NIR-PIT enhances therapeutic efficacy by generating local and systemic antitumor immunity [90]. Maruoka et al. combined CD44-targeted NIR-PIT with a PD-1/PD-L1 axis blockade [102], cytotoxic T lymphocyte-associated antigen-4 (CTLA4) blockade [103], or IL-15 treatment [104]. These three combinations showed enhanced antitumor effects compared with each therapy alone. Notably, CD44-targeted NIR-PIT combined with CTLA4 or PD-1/PD-L1 blockade showed varying antitumor responses in different tumor models. For instance, in MOC1 tumors, 44% complete remission (CR) was achieved with NIR-PIT and CTLA4 blockade, compared with only 8% when using NIR-PIT and PD-1 blockade. However, in MC38-luc tumors, a CR of only 11% was achieved with NIR-PIT and CTLA4 blockade, while a CR of 70% occurred with NIR-PIT and PD-1 blockade [103]. Given the heterogeneity of immune cell populations in different tumors, the most appropriate immune checkpoint inhibitors to be combined with NIR-PIT could be selected based on the expression level of specific immune checkpoint molecules in the respective tumors. For instance, Hartmans et al. combined tyrosine kinase inhibitors (TKIs) and EGFR/HER2-targeted NIR-PIT and found that TKIs upregulate EGFR expression, which contributes to the improved antitumor effect [51]. In addition to the combinational strategy, cytotoxic drugs can be directly incorporated into TSPCs by constructing antibody-drug conjugates (ADCs). Trastuzumab emtansine (T-DM1) is an ADC comprising the anti-HER2 monoclonal antibody trastuzumab conjugated with the cytotoxic drug maytansinoid DM1 [48]. In this way, a T-DM1-IR700 conjugate with two different cell-killing payloads was developed that enhances cytotoxicity in vitro and antitumor effects against large tumor xenografts (~200 mm3) in vivo.

Potential application of NIR-PIT for cancer treatment

In this section, we review the application of NIR-PIT in cancer treatment (Figure 4 and Tables 1-3). The targets, efficacy, and NIR-PIT approach are described for each cancer type. To assist in locating each cancer type, they have been arranged alphabetically.

B-cell lymphoma

CD20 is a B-cell specific antigen highly expressed in many B cell lymphomas (BLs) and was, therefore, selected as an important target for treating BL [105]. The lack of shedding or internalization makes CD20 an excellent candidate for NIR-PIT. Anti-CD20 NIR-PIT has shown efficacy in both indolent and aggressive BLs. In vitro studies suggest that aggressive BL is more sensitive to NIR-PIT than indolent BL. In animal models using two human aggressive BL cell lines (Daudi and Ramos), NIR-PIT significantly inhibits tumor growth and prolongs survival compared with control groups [65]. For aggressive BL, NIR-PIT elicits a strong therapeutic response than radioimmunotherapy, which has been proven effective in mice bearing Ramos tumor grafts. [66, 106]. However, NIR-PIT is difficult to implement for treatment of other hematological malignancies without formation of a local mass.

Bladder cancer

EGFR is highly expressed in 74% of bladder cancer tissue specimens [28]. NIR-PIT using the anti-EGFR antibody panitumumab led to inhibition of tumor growth in a mouse model and completely shrank the tumor size in three out of ten xenograft mice. Moreover, the outcome of NIR-PIT correlates with the level of EGFR expression on tumor cells [36].

Researchers have also made efforts to develop a larger animal model for translational studies and to investigate cystoscopy for NIR light delivery. As spontaneous bladder cancer in dogs closely mimics human invasive bladder cancer [107], a canine version of the anti-EGFR antibody cetuximab, can225IgG linked to IR700 (can225-IR700), was tested in a canine tumor-bearing xenograft mouse model. A single injection of can225-IR700 with two rounds of light exposure led to remarkable therapeutic response [38].

HER2 is a member of the tyrosine kinase EGFR family that promotes cell proliferation, differentiation, and motility. It is commonly overexpressed on various types of cancer cells, and has been identified in 40% of bladder cancer specimens [108]. Moreover, a cocktail of NIR-PIT performed significantly better targeting EGFR and HER2 together, than when targeting either alone, both in vitro and in vivo. However, 4/11 mice died within 48 h following irradiation with the higher dose light (100 J/cm2) in the combination group. Indeed, excessive light exposure or TSPC dosage beyond the maximum tolerated doses (MTDs) can result in death of mice. Thus, the potential treatment toxicity with drug dose-escalation must be considered to achieve the optimal antitumor response. Subsequent toxicity assays indicate that tumor lysis syndrome (TLS), caused by massive tumor necrosis, provoked a strong systemic inflammatory response in the mice that died [86]. However, in clinical trials, no incidence of TLS was observed in cancer patients treated with NIR-PIT [109, 110]. Moreover, aggressive intravenous hydration and administration of hypouricemic agents significantly decrease the incidence of poor outcomes with TLS [111]. Nevertheless, a balance must be achieved between optimum antitumor effects and minimal toxicity to healthy tissues prior to clinical application.

CD47—a ligand that transmits inhibitory signals for macrophage phagocytosis—is overexpressed on bladder cancer cells, however, is absent in the normal luminal urothelium [112]. Anti-CD47 NIR-PIT causes cell necrosis in patient-derived bladder cancer cells and human bladder cancer cell lines, and significantly inhibits tumor growth in a xenograft model [75]. Furthermore, NIR-PIT does not compromise anti-CD47 antibody binding and has the potential to enhance phagocytosis by increasing the surface expression of calreticulin, which can counterbalance the inhibitory signal of CD47 [75, 113].

Bone cancer and bone metastasis

Nakamura et al. chose an explanted bovine rib as a model to determine whether NIR-PIT can effectively kill cancer cells within bones. Their ex vivo experiments showed that although the transmittance of NIR light was only 4.52%, this therapy could potently destroy cancer cells behind the rib [114]. Meanwhile, given that human bone samples have been reported to have a markedly lower density than bovine ribs, NIR-PIT should theoretically perform better in human bone cancer [115].

Brain cancer

Considering that EGFR is overexpressed in approximately 50% of primary glioblastomas (GBMs), it represents an interesting target for GBM therapy [29]. Burley et al. developed a low-molecular-weight (~7 kDa) EGFR-specific affibody to be conjugated with IR700 (ZEGFR:03115-IR700DX). This conjugate showed high specificity for binding to EGFR and significant therapeutic efficacy after light irradiation, both in vitro and in a xenograft model of GBM [33].

G protein-coupled receptors (GPCRs), including US28 and type 2 cannabinoid receptor (CB2R), are abnormally overexpressed in multiple tumors, including brain tumors [116, 117]. Meanwhile, a nanobody specifically binding to the extracellular region of US28 was developed to be conjugated with IR700, which potently mediated NIR-PIT killing of US28-expressing glioblastoma cells both in 2D and 3D cultures after NIR exposure [92]. NIR-PIT treatment with mbc94, a CB2R-specific molecule, was efficient and target-selective in delaying the growth of brain tumor cells both in vitro and in a xenograft model [118, 119].

CSCs are undifferentiated tumor cells with high self-renewal capacity, limited differentiation capability, and high motility [120, 121]. CD133 is a CSC marker in many tumor types, including GBM [122]. Hence, NIR-PIT with anti-CD133-IR700 was tested in a mouse xenograft model using orthotopic gliomas initiated from patient-derived CSCs. The treatment was highly effective and prolonged survival in the orthotopic model, although cerebral edema was observed several hours after NIR-PIT, which may have been due to tumor necrosis [74].

Breast cancer

HER2 is highly expressed in approximately 20% of human breast cancers and is generally associated with poor outcomes [123]. Thus, HER2-targeted NIR-PIT may prove to be a promising option for breast cancer treatment. Compared with the intact antibody, the small affibody (6-7 kDa) exhibits superior tumor penetration and rapid clearance. Meanwhile, the HER2 affibody-IR700 conjugate leads to necrotic cell death only in HER2-overexpression cancer cells following NIR light irradiation, without causing collateral damage to HER2 low-expressing cancer cells [93, 124]. In addition, combination of the HER2 affibody-based conjugate and the trastuzumab-IR700 conjugate induces a strong therapeutic effect against HER2-positive cancer cells, including HER2 low-expressing cells, trastuzumab-resistant cells, and brain metastatic breast cancer cells [125].

Anti-EGFR-based NIR-PIT has also been investigated for the treatment of breast cancer. In an orthotopic breast tumor model, panitumumab conjugated with IR700 for NIR-PIT immediately induced the killing of >90% cells in vivo after irradiation at a high light dose (>100 J/cm2) [50]. Nani et al. used an NIR photocaged-group-based ADC, CyEt-panitumumab-duocarmycin, to selectively trigger the release of the cytotoxic drug duocarmycin after NIR-PIT to enhance tumor-specific therapeutic effects [126]. In this study, NIR-triggered drug release was achieved after panitumab-mediated NIR-PIT, and the SUPR effects contributed to the increased ADC tumor accumulation and high tumor-background ratio [47].

TNBC is defined by the lack of expression of estrogen receptor (ER), progesterone receptor (PR), and HER2, and is associated with shorter relapse-free intervals and lower overall survival than other subtypes. Moreover, no targeted therapy is currently available for TNBC, in contrast to ER-positive or HER2-positive breast tumors. Fortunately, 50-70% of TNBCs express EGFR [127] and the anti-EGFR antibody cetuximab (cet)-IR700-based NIR-PIT has demonstrated the ability to suppress tumor growth and significantly prolong patient survival regardless of the EGFR expression level in the tumors, although higher expression of EGFR induces increased cell death. Moreover, the therapeutic effect can be further enhanced by splitting the TSPC dose and repeating the NIR exposure [46].

A cocktail of NIR-PIT targeting three different receptors has also been investigated for the treatment of TNBC. These receptors, including EGFR, EpCAM, and chondroitin sulfate proteoglycan 4 (CSPG4), are abundantly expressed in TNBC, but only at low levels in normal tissues [128-130]. The combination of all three scFv-IR700 conjugates increases phototoxicity by up to 40% compared with each photoimmunotherapy agent in vitro [49].

The high recurrence and metastasis rates of TNBC are due in part to the presence of CSCs, which are resistant to conventional therapy [129]. Moreover, CD44 is a CSC marker in many cancer types, including breast cancer [131], and its overexpression results in a more aggressive phenotype [132]. Hence, the conjugation of anti-CD44 antibody and IR700 induces marked tumor shrinkage and significant cell death in CD44-positive breast cancer cell populations following light exposure [72]. However, hypoxia can upregulate CD44 expression [133] and induce cancer cell resistance to antitumor therapies, such as radiation and chemotherapy. Nevertheless, hypoxia does not compromise NIR-PIT efficacy, which destroys the cell membrane without the need for oxygen [72]. Indeed, NIR-PIT using T-DM1-IR700 (an ADC-based TSPC) exhibits superior antitumor effects in HER2-positive large tumor models even when NIR light could not sufficiently irradiate the whole large tumor [48].

Cholangiocarcinoma (CCA)

Type I transmembrane protein tumor-associated calcium signal transducer 2 (TROP2) has been tested as a therapeutic target in pancreatic carcinoma (PC) and CCA. NIR-PIT with an anti‐TROP2 antibody significantly inhibits PC and CC xenograft tumor growth. In the same study, TROP2 was identified in 40% of PC samples and 46% of CCA samples, indicating that half of the patients could be covered [134]. However, light delivery may be hindered in CCA due to its location within the body. Accordingly, Hirata et al. developed an NIR-LED catheter intended for use within the bile duct, equipped with a temperature sensor to prevent thermal burns. NIR-PIT, using the novel catheter device inserted under subcutaneous tumor xenografts in mice, exhibits therapeutic effects comparable to those of the external irradiation device. However, this device has not been assessed in the murine bile duct for CCA treatment due to the small size of murine bile ducts [135].

Colorectal cancer (CRC)

Elekonawo et al. evaluated the effects of CEA-targeted NIR-PIT in nine CRC cell lines expressing different levels of CEA. Their results indicate that the antitumor efficacy of NIR-PIT is proportional to CEA expression level [136]. Another group investigated CEA-targeted NIR-PIT in an orthotopic colon cancer mouse model and observed clear inhibition of tumor growth [58].

Moreover, glycoprotein A33 antigen (GPA33), which is overexpressed in human CRC, has also been investigated as a target for NIR-PIT [137]. The single-chain variable fragment of an antibody against GPA33 (A33scFv) conjugated to IR700 (A33scFv-IR700) exhibits rapid and specific killing of GPA33-positive colorectal tumor cells after NIR laser light irradiation in vitro. In mice bearing GPA33-positive tumor xenografts, A33scFv-IR700-mediated NIR-PIT exhibits significant antitumor effects after a single NIR exposure [138]. Platelet-derived growth factor receptor β (PDGFRβ) can be used in NIR-PIT for colon cancer as it is abundantly expressed on the pericytes of various types of tumors [139]. Although PDGFRβ is also expressed in normal tissues, it is safe to use as a target as endothelial cells completely cover the pericytes in normal blood vessels. Meanwhile, endothelial cells irregularly cover the vascular wall only in tumor blood vessels, permitting the delivery of PS to exposed pericytes [140]. Moreover, PDGFRβ-specific dimeric affibody is cheaper and smaller than a full length-antibody. NIR-PIT using this affibody successfully damages tumor blood vessels, leading to thrombosis or even vessel occlusion. In addition, the resulting intensified tissue hypoxia helps destroy the tumor [78].

Since EGFR is also highly expressed in most CRC cells, an EGFR-targeting affibody was utilized as an NIR-PIT agent combined with long-acting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) to treat chemotherapy MDR and TRAIL resistance in CRC. In this study, EGFR-targeted NIR-PIT significantly increased the sensitivity of MDR cells to TRAIL, and the combination therapy efficiently eradicated large CRC xenografts (~150 mm3) with both chemotherapy MDR and TRAIL resistance [141].

Cutaneous squamous cell carcinoma (CSCC)

NIR-PIT is suitable for the treatment of skin disorders as the light can be directly irradiated. In the case of CSCC, EGFR [19, 20, 27, 45, 97] and mesothelin [142] have been used as targets for TSPCs, which specifically recognize and kill A431 cells after exposure to NIR light, both in vitro and in xenograft-bearing mice. Moreover, an open-label clinical trial administering cetuximab-mediated NIR-PIT combined with PD1 blockade to patients with advanced or metastatic CSCC is currently underway (NCT04305795).

Esophageal cancer (EC)

Growth factor receptors, including EGFR and HER2, can act as targets of NIR-PIT in EC. TSPCs targeting these growth receptors showed good phototoxicity in vitro. Researchers have also found that the therapeutic effects of NIR-PIT can be further enhanced using a TKI to upregulate the expression of growth factor receptors [51]. Many studies have reported that CAFs are associated with therapeutic resistance in EC. In fact, CAFs have been identified as a crucial component of the TME that contributes to tumor progression [143, 144]. Meanwhile, anti-FAP NIR-PIT showed safe and promising inhibitory effects on fibroblast activation protein (FAP, a specific marker of CAFs [145])-positive CAFs in vitro and in vivo [76]. Another study showed that the coculture of EC cells and CAFs results in a CAF-driven resistance to chemotherapy. Moreover, a combination of 5-FU with CAF-targeted NIR-PIT can result in a 70.9% drug-resistant tumor shrinkage, whereas treatment with 5-fluorouracil (5-FU) alone only elicits 13.3% tumor shrinkage [146]. These results suggest that NIR-PIT-targeting CAFs has the potential to overcome resistance to conventional chemotherapy.

Gastric cancer

Several studies have tested NIR-PIT to treat peritoneal dissemination of gastric cancer because NIR light can be distributed to multiple cancer sites simultaneously in the peritoneal cavity via endoscopy. Given that approximately 20% of gastric cancers express HER2, these studies utilized anti-HER2 NIR-PIT [30, 31]. NIR-PIT using a fiber optic diffuser showed good therapeutic effects in a mouse model of disseminated peritoneal gastric cancer. Meanwhile, histological analysis revealed diffuse cancer cell death in treated tumor tissues, and prolonged survival following repeated NIR-PIT [54, 55]. This strategy might be easier to execute in human patients than mouse models due to the greater flexibility associated with performing endoscopies in the larger peritoneal space of humans. Moreover, compared with NIR-PIT alone, the addition of 5-FU to HER2-targeted NIR-PIT elicits significant cytotoxicity in both short-term and long-term follow up. The enhanced and prolonged tumor growth inhibition in vivo can be attributed to the difference in cell killing mechanisms, namely, necrotic membrane damage and apoptotic cell death [52].

Gastrointestinal stromal tumors (GISTs) are the most common type of submucosal tumors of the gastrointestinal tract, with up to 90% of GISTs overexpressing c-KIT (a receptor tyrosine kinase also known as CD117) [147]. Thus, anti-c-KIT-based NIR-PIT has been used for the imaging and treatment of GISTs. Tumor-specific accumulation of IR700-conjugated anti-c-KIT antibodies has been observed in mice bearing subcutaneous GIST xenografts, while GISTs have been visualized using fluorophore AF48-conjugated anti-c-KIT antibody through the normal mucosa of an orthotopic rat model. Additionally, effective cell killing has been reported in vitro and in mouse models [148]. Thus, the IR700-conjugated anti-c-KIT antibody could be a promising theranostic agent for GISTs.

VEGFR-2 expressed on the vascular endothelium has also been evaluated as a potential target of NIR-PIT for gastric cancer. In fact, anti-VEGFR2-mediated NIR-PIT induces damage in the tumor neovasculature of xenograft tumors. Compared with anti-HER2 NIR-PIT, VEGFR2-targeted NIR-PIT has a stronger antitumor effect, although this approach leads to a lower amount of TSPC inside the tumor. However, vessel destruction may fail to induce SUPR effects and the subsequent immune response achieved with anti-HER2 NIR-PIT. Moreover, although VEGFR2 is also expressed in normal tissues, NIR light can be applied locally, thus limiting normal tissue toxicity [56].

Head and neck cancer

Due in part to the complex topography of the regional anatomy and the abundance of vital structures, the recurrence rate of squamous cell carcinoma of the head and neck (SCCHN) remains high even after administration of the gold standard treatment, i.e., surgical resection plus adjuvant radiotherapy, or chemoradiation [149]. Meanwhile, EGFR-targeted NIR-PIT has the potential to eliminate microscopic tumor remnants in a postsurgical setting. In a study performed by Moore et al., NIR-PIT using pan-IR700 was investigated in vitro, and in an in vivo model of residual disease after incomplete resection. Mice that received surgical treatment plus adjuvant NIR-PIT showed a significant (P < 0.001) reduction in tumor regrowth 30 days post-NIR-PIT in both the 50% and 90% subtotal resection groups. However, tumor recurrence ultimately occurred in all treated mice. Hence, a more potent NIR-PIT regimen, such as repeated light exposure, may be needed [34]. In addition to the full antibody, 7D12, a small nanobody targeting EGFR has been used in NIR-PIT for head and neck cancer. The 7D12 nanobody has a shorter half-life and deeper tumor penetration than the intact antibody. 7D12-mediated NIR-PIT resulted in significant tumor necrosis and infiltration of immune cells within 1 h of NIR-PIT, however, showed no toxicity to normal tissues [35]. CD44 is a CSC marker that is selected for treatment of oral cavity squamous cell carcinoma. A single injection of the CD44-targeted TSPCs followed by light exposure induced a potent antitumor effect in vivo [73].

Hepatocellular carcinoma (HCC)

Glypican-3 (GPC3) is an attractive antigen for treating HCC as its expression in HCC is higher than that in normal liver [150]. Hanaoka et al. compared NIR-PIT using the whole IgG antibody anti-GPC3 YP7 with that using the human heavy-chain antibody anti-GPC3 HN3 and found that both groups showed significant inhibition of tumor growth in vivo. However, the HN3-IR700 conjugate provided a deeper, and more homogeneous, distribution in tumors thanYP7-IR700, without compromising the affinity of HN3 for GPC3. Moreover, HN3-IR700 retained its capacity to induce antibody-dependent cell-mediated cytotoxicity (ADCC). Hence, researchers may prefer to use HN3 rather than YP7 in NIR-PIT for HCC treatment. Considering that light delivery can be problematic in HCC, light probes placed through vessels or inserted directly through the skin into the liver (such as conventional interventional angiography or focal laser ablation) could be necessary for effective NIR-PIT [59]. Additionally, YP7-mediated NIR-PIT in combination with albumin nanoparticle-bound paclitaxel has been shown to increase the antitumor efficacy via SUPR effects [60].

Lung cancer

The lungs are considered suitable for light therapy due to the relative ease of light penetration into deep air-pockets without significant light attenuation. In a transgenic model of spontaneous lung cancer, tumor growth was significantly inhibited in the anti-EGFR NIR-PIT group compared with the control group (P < 0.01 at all time points) [40]. In another mouse model of pleural disseminated non-small-cell lung carcinoma, anti-HER2 NIR-PIT led to a significant reduction in tumor volume (P = 0.002 vs. TSPC) and prolonged survival (P < 0.0001) [42]. Delta-like protein 3 (DLL3) was also selected as a potential target for small cell lung cancer (SCLC). Moreover, anti-DLL3 NIR-PIT led to extensive cell death in vitro and a remarkable decrease in tumor volume in a xenograft model [151]. PD-L1 is highly expressed in many cancers, leading to suppression of T-cell activation and unimpeded tumor growth [152]. In addition, PD-L1 overexpression is associated with poor prognosis [153]. Hence, the anti-PD-L1 antibody has also been used as an NIR-PIT carrier. PD-L1-targeted NIR-PIT induces significant inhibition of tumor growth and prolonged survival in a PD-L1-expressing lung papillary adenocarcinoma model, even with a single treatment [154].

Considering that the lungs are among the most common sites for developing metastases, NIR-PIT has also been used to treat lung metastases. In fact, in a murine model, anti-HER2 trastuzumab-based NIR-PIT demonstrated a significant reduction in early-stage lung metastatic disease (P < 0.0001) without damaging non-targeted tumors or normal lung tissues [41]. Moreover, a significant reduction in tumor size and prolonged survival was achieved using NIR-PIT in a mouse model with large lung metastases of ~100 mm3 in volume [39]. When translated into humans, bronchoscopy or direct thoracoscopy may be required to deliver NIR light to reach the entire lung volume. Moreover, as pulmonary metastasis is a systemic disease, NIR-PIT could be applied as a palliative treatment to relieve symptoms, which may lead to longer survival and a better quality of life.

Malignant pleural mesothelioma (MPM)

The poor prognosis and few available therapeutic approaches for MPM have attracted interest in the possible application of NIR-PIT in MPM. Podoplanin (PDPN), a type I transmembrane glycoprotein, is used as a specific pathological diagnostic marker for MPM. Anti-PDPN antibody NZ-1-mediated NIR-PIT induces light-dose-dependent cytotoxicity in vitro and good therapeutic effects in vivo, in both flank and orthotopic xenograft models [155]. However, NZ-1 also binds to normal lymphatic endothelial cells and can potentially harm normal cells. Hence, a novel mAb (CasMab) has been developed to distinguish cancer cells from normal cells by recognizing differential expression of glycosylated PDPN [156]. Thus, NIR-PIT employing CasMab may achieve a superior antitumor effect in the treatment of MPM.


Melanoma is one of the most fatal types of skin cancer. Given that CD146 is upregulated in melanomas and is associated with progression and metastasis [157], anti-CD146 NIR-PIT has been applied to xenograft melanoma tumors of varying sizes. Compared with the control group, after treatment with anti-CD146 NIR-PIT, significant suppression of tumor growth was observed in small xenografts (86.48 ± 14.80 mm3), whereas no significant difference was detected in large xenografts (330.80 ± 36.12 mm3) [67]. The poor therapeutic response elicited by NIR-PIT for the treatment of large melanomas may be due to the finite TSPC delivery and limited NIR light penetration into tumors [158]. Irradiation of large tumors can be performed using optical fibers inserted through needle catheters, which have been applied to treat head and neck squamous cell carcinoma (HNSCC) patients in clinical trials [109]. Moreover, repeated NIR exposure can improve the delivery of TSPC into the tumor bed via SUPR effects [24]. Additionally, combinatorial therapies comprising NIR-PIT and conventional immunotherapy may achieve a synergistic suppressive effect on tumor growth for melanomas of larger volumes [8].

Furusawa et al. investigated the efficacy of CD29-targeted NIR-PIT in the highly pigmented melanoma model. CD29 (also named integrin beta-1, ITGβ1) is a cell surface glycoprotein that is expressed on both melanoma and cytotoxic T cells. CD29-targeted NIR-PIT efficiently suppresses tumor growth in the pigmented melanoma model. More importantly, NIR-PIT does not cause damage to immune cells at the treatment site while preferentially killing proliferative cancer cells. Moreover, the combination of an anti-CTLA4 antibody and CD29-targeted NIR-PIT increases the infiltration of CD8+ T cells, resulting in greater tumor growth inhibition with prolonged survival [159].

Virus-like particles (VLPs) and IR700 have been employed to construct a TSPC (AU-011) for the treatment of primary uveal melanoma, leading to significant dose-dependent tumor necrosis after NIR irradiation. According to Kines et al., VLPs were prepared based on the HPV capsid protein. VLP attachment and internalization by targeted cells is mediated by heparan sulfate proteoglycan (HSPG) expressed on multiple tumor cell types. VLPs have two advantages over antibodies for NIR-PIT treatment. First, they exhibit multivalent binding, which enables stronger interaction with target cells. Second, a large number of IR700 dye molecules can be readily conjugated to a single VLP, leading to a higher concentration of PS on the cell surface [95].

Mycosis fungoides (MF)

MF is a low-grade T-cell lymphoma, accounting for approximately 53% of primary cutaneous lymphomas [160]. Few therapies are available for MF treatment; therefore, new therapeutic approaches are necessary. A study by Silic-Benussi et al. investigated anti-cutaneous lymphocyte antigen (CLA)-targeted NIR-PIT, which effectively destroyed MF-derived My-La CD4+ cells in vitro [161].

Ovarian cancer

Patients with intraperitoneal tumor dissemination of ovarian carcinoma, commonly present at an advanced stage and have a poor prognosis [162]. However, the antitumor effect of anti-HER2 NIR-PIT was investigated in a mouse model of disseminated peritoneal luciferase-expressing ovarian cancer [43, 163] and was found to induce destruction of a large fraction of the tumor cells (NIR-PIT vs. control at day 7, P = 0.0037) [43]. Galactosyl serum albumin (GSA) is a surface lectin that can bind weakly to beta-D-galactose receptors overexpressed in ovarian cancer [164]. Although GAS-mediated NIR-PIT has been evaluated in disseminated peritoneal ovarian cancer, it appears to be less potent than antibody-mediated therapy. Two possible reasons for this might be the weak binding affinity of GSA to cancer cell membranes and the massive internalization of GSA-IR700 by cells. However, when no specific antibody is available, GSA may be a useful alternative target molecule. Moreover, repeated anti-GSA NIR-PIT treatment could help improve tumor suppression by promoting deeper penetration of GSA-IR700 into tumors following the initial treatment [163].

Integrins are transmembrane cell adhesion proteins that can act as signaling receptor proteins to regulate cancer progression by binding to extracellular matrix (ECM) components. Additionally, some integrins are overexpressed in ovarian cancer, neovasculature, and on immunosuppressive lymphocytes [165]. Approximately half of integrins bind to the ECM and cytoskeleton by recognizing the RGD sequence. Thus, RGD-containing peptides are used as carriers for targeting cancers [166]. In fact, an RGD-based NIR-PIT targeting integrins exhibits strong phototoxicity in a 3D model of ovarian cancer cells [79]. Since immunosuppressive lymphocytes contribute to a poor prognosis, NIR-PIT might further improve the therapeutic response by eliminating these immunosuppressive cells within the TME [167].

Pancreatic cancer

Pancreatic cancer cells express high levels of CEA [168]. CEA-targeted NIR-PIT induces extensive cell death and a significant reduction in tumor size and weight in an orthotopic mouse xenograft model. However, the tumor eventually recurred in all treated mice, likely due to the administration only a single round of treatment [169]. CEA-mediated NIR-PIT has also been used as a postsurgical treatment for the prevention of local and metastatic recurrence. In an orthotopic mouse model with a human pancreatic cancer cell line, bright light surgery (BLS) plus NIR-PIT decreased the local and metastatic recurrence rate to 1/7 mice (P = 0.001) and 2/7 mice (P = 0.03), compared with 7/7 mice and 6/7 mice respectively, in the BLS alone group. Significant differences in the tumor growth rate, tumor size, and tumor weight between the two groups persisted even several weeks after surgery [57]. NIR-PIT was also effective when applied in a patient-derived orthotopic xenograft (PDOX) mouse model. The local recurrence rate was reduced from 85.7% in the BLS group to 28.6% in the BLS and NIR-PIT combination group (P = 0.05). Moreover, the average weight of recurrent tumors was significantly lower in BLS+PIT-treated mice than in the BLS alone group (P = 0.015) [170].

Cadherin-17 (CDH17 or CA17), a calcium-dependent transmembrane glycoprotein, is highly expressed in many gastrointestinal cancers including pancreatic adenocarcinoma. CDH17-targeted NIR-PIT significantly inhibits tumor growth in a pancreatic adenocarcinoma xenograft model after three cycles of treatment [171].

Prostate cancer (PCa)

Prostate-specific membrane antigen (PSMA) is an attractive target for the treatment of PCa using NIR-PIT as it is highly expressed in prostate cancer cells and in the neovasculature. PCa can be illuminated with NIR light either directly or via a transurethral catheter [62]. Different tumor-homing ligands have been used to construct TSPCs for PCa treatment, including antibodies, Db, Mb, and small-molecule agents. Whole IgG-mediated NIR-PIT has been shown to strongly inhibit tumor growth and improve overall survival in a xenograft mouse model [62, 63, 89]. Meanwhile, Db, Mb, and whole IgG-based TSPCs display similar antitumor effects at the same molar concentration. However, the smaller Dbs allow the shortest interval between TSPC injection and light exposure, which may have advantages in the clinical translation of photoimmunotherapy [89]. Moreover, a PMSA-targeted TSPC, i.e., YC-9, was constructed by conjugating IR700 to the PMSA-binding small molecule Lys-Glu-urea through a lysine-suberate linker. YC-9 exhibits PSMA-specific cell killing, resulting in delayed tumor growth [61]. Moreover, to apply NIR-PIT in surgery, Derks et al. developed a novel agent by incorporating radionuclide indium-111 into Lys-Glu-urea-IR700 TSPC to optimize the multimodal image-guided PCa surgery via combination with targeted NIR-PIT. This novel design could assist in locating both distant tumor lesions and metastatic lymph nodes, while also facilitating the removal of residual tumors without damaging important structures, such as the nerves controlling the urinary bladder [94].

NIR-PIT clinical studies/trials in humans

The potential efficacy and safety of NIR-PIT make it a promising modality for cancer therapy, leading to its evaluation in various clinical trials worldwide. More specifically, in the United States (US), a phase I/IIa clinical trial of NIR-PIT based on a conjugate comprising cetuximab and IR700 (cetuximab-IR700, RM-1929) has recently concluded. Results indicate that a manageable safety profile and substantial antitumor responses were obtained in locoregional, recurrent HNSCC patients who had previously exhibited unsatisfactory therapeutic responses to traditional clinical therapy [109]. In Japan, a phase I, open‑label, single‑center study of NIR-PIT (RM‑1929) in patients with recurrent HNSCC showed a similar safety profile and promising antitumor activity to that observed in the American phase I/IIa study [110]. Given these clinically meaningful results, a global phase III trial including 275 patients with recurrent or second primary HNSCC is currently underway (NCT03769506). In September 2020, cetuximab-IR700 was approved by the Japanese government for the treatment of unresectable locally advanced or recurrent HNSCC [172]. In December 2020, an open-label, phase I/II tumor immunotherapy trial was initiated using RM‑1929-mediated NIR-PIT in combination with anti-programmed cell death protein 1 (PD1) immune checkpoint inhibitors in patients with recurrent or metastatic HNSCC, or advanced or metastatic CSCC (NCT04305795). Furthermore, based on encouraging results in animal models, a phase Ib/II clinical trial is currently underway in the US to investigate the safety, immunogenicity, and efficacy of AU-011-mediated NIR-PIT in patients with small primary choroidal melanomas (NCT03052127).

NIR-PIT for non-malignant conditions


The increasing prevalence of antimicrobial-resistant bacteria necessitates the development of alternative antimicrobial strategies, including targeted antimicrobial photodynamic therapy (aPDT). Bispo et al. used an antibody against the immunodominant staphylococcal antigen A (IsaA) for IR700 conjugation, which successfully targets and destroys methicillin-resistant Staphylococcus aureus (MRSA) cells after light irradiation. With the help of the enhancing agent potassium iodide, the anti-IsaA NIR-PIT can eliminate a high MRSA titer, regardless of the strong antioxidant activity of plasma [173]. Moreover, Yasui et al. used low-cost polyclonal chicken egg yolk IgY antibodies for the construction of a TSPC to kill Candida albicans. In a cutaneous infected mouse ulcer model, TSPC led to skin recovery after NIR irradiation with minimal damage to the uninfected epithelium [174]. Reessing et al. described a general strategy for the conjugation of IRdye800CW/IR700 with the small-molecule antibiotics vancomycin and amphotericin B. Vancomycin-IRdye800CW conjugates can specifically bind to gram-positive bacteria, not gram-negative bacteria. The vancomycin-IR700 conjugates retain the ability of IR700 to produce reactive oxygen species, facilitating targeted action against invading pathogens [175]. Meanwhile, as an antiviral treatment, Sadraeian et al. used NIR-PIT to kill HIV-infected cells by targeting the Gp41 glycoprotein, a component of the HIV envelope spike (Env). This anti-Gp41 therapy elicits cytotoxicity in HIV-env-expressing cells [176]. However, in general, microbial growth is greater than that of cancer cells. In this regard, the amount of TSPC and the NIR light energy may need to be increased to achieve better therapeutic efficacy.


Innocuous touching provokes severe pain in patients with mechanical allodynia, a major symptom of neuropathic pain. Peripheral sensory neurons expressing receptor tyrosine kinase B (TrkB) can sense the lightest touch under basal conditions and have been identified as necessary for mechanical allodynia in mice after nerve injury. In models of neuropathic pain, brain-derived neurotrophic factor (BDNF, a ligand for TrkB) selectively mediated NIR-PIT and effectively eliminated TrkB-positive sensory fibers, thus reversing mechanical allodynia in multiple types of neuropathic pain [177]. Another attractive target for reducing pain is nerve growth factor (NGF) as this ligand, and its receptor TrkA/p75, play critical roles in the development and function of peripheral nociceptive neurons [178]. Accordingly, NGF derivatives conjugated with IR700 selectively photoablate TrkA-positive nociceptors and substantially reduce the response to point-based nociceptive mechanical stimuli after irradiation of the injected area. Moreover, photoablation using NGF derivatives also abolishes long-term mechanical hypersensitivity in mouse models of inflammatory, osteoarthritic, and neuropathic pain. Notably, the behavioral responses to dynamic mechanical stimuli are not altered by NGF-mediated photoablation, as these dynamic stimuli are transduced by TrkB-positive mechanoreceptors after nerve injury [179].

In summary, NIR-PIT can directly attack cells to bypass the complex signaling pathways underlying pain responses. Moreover, the TSPC dosage, light intensity, and frequency of application are adjustable, thus allowing for personalized therapeutic regimens. Indeed, the long-lasting efficacy of a single photoablation treatment regimen has the potential to reduce toxicity compared with other therapies.


Chronic itch causes an urgent desire for scratching and subsequent skin damage, which is not only difficult to treat, but also impacts the quality of life to the same extent as pain [180]. The cytokine interleukin (IL)-31 has been identified as a key trigger in mediating the itch response [181]. In fact, a single treatment with IL-31 receptor-targeted NIR-PIT can induce long-lasting relief of itch-associated behavior in mouse models of atopic dermatitis and familial primary localized cutaneous amyloidosis (FPLCA), thus facilitating skin healing. The advantages and underlying mechanisms of NIR-PIT for treating itch are similar to those summarized above for pain; the mechanism is reportedly associated with the retraction of itch-sensing neurons from the epidermis [182].

Hyperinsulinemic hypoglycemia

Uncontrolled production of insulin by pancreatic β-cells can cause hyperinsulinemic hypoglycemia [183]. The GLP-1 receptor (GLP-1R) is expressed in pancreatic β-cells and in virtually all benign insulinomas [184]. As expected, NIR-PIT employing an analog of GLP-1 can specifically bind and kill GLP-1R-positive cells, providing a new, effective, and minimally invasive approach to normalize blood glucose levels in patients with hyperinsulinemic hypoglycemia [185].

Rheumatoid arthritis (RA)

Fibroblast-like synoviocytes (SFs) are a unique cell type that distinguishes RA from other inflammatory diseases affecting the joints [186]. FAP is a surface marker for activated SFs. The upregulation of FAP on activated SFs reportedly correlates with the long-term progression of RA [187]. Dorst et al. applied NIR-PIT to target FAP in a mouse model of RA and reported that the development of arthritis was delayed by selectively depleting FAP-positive fibroblasts in mice with collagen-induced arthritis [188]. In addition, FAP-based NIR-PIT was found to kill FAP-positive fibroblasts within a population of primary synovial fibroblasts, and biopsies from human RA synovial tissue. [189]. This novel FAP-mediated approach may have potential for treating RA, as an alternative to immunosuppressive drugs.

Systemic sclerosis (SSc)

SSc, also called scleroderma, is a complex autoimmune disease characterized by inflammation, vasculopathy, and fibrosis of the skin and internal organs. This fibrosis is partially caused by the presence of activated myofibroblasts (myo) in the tissues [190]. Similar to RA, FAP is specifically expressed on activated fibroblasts in SSc; thus, FAP-targeted NIR-PIT has the potential to eliminate pathological fibroblasts in SSc skin. In fact, FAP-mediated NIR-PIT could deplete primary skin fibroblasts from SSc patients in 2D/3D cultures and fully prevented the contraction of SSc fibroblasts in 3D cultures, which is critical for tissue stiffness and fibrosis [191].

Elimination of target cells from mixed cell cultures or tissues

It is desirable, yet difficult, to eliminate a specific type of cell from mixed cell cultures or tissues without damaging adjacent cells. By employing targeted antibodies and spatially selective irradiation, NIR-PIT could eliminate specific cells from mixed 2D/3D cell cultures or tissues ex vivo, and from mixed populations in tumor models without damaging non-targeted cells [192, 193]. This technique could remove potential interference and contamination by unwanted cells.

Limitations and challenges of NIR-PIT

Despite the very promising therapeutic effects, a few potential limitations and challenges remain when considering NIR-PIT implementation. First, light exposure can cause potential toxicity to organs located in proximity to the tumor. In fact, mild peri-tumor dermal edema is one of the most common treatment emergent adverse events (TEAE) reported in patients treated with NIR-PIT. Meanwhile, low-grade, reversible skin photosensitivity was observed in a small proportion of treated patients [109, 110]. Moreover, exposure of liver or kidney metabolic organs to light might also lead to damage. Second, as the penetration light depth is limited and illumination is localized, it is difficult for NIR-PIT to treat all disseminated sites or those located in deep organs or tissues where the light irradiation is not accessible. However, since IR700 emits near-infrared fluorescence, optical imaging can help to map and irradiate the primary and metastatic tumors. Meanwhile, NIR-PIT using an endoscopic fiber optic diffuser is a promising candidate for the treatment of disseminated peritoneal metastases [54, 55]. Moreover, the combination of NIR-PIT and immune-checkpoint inhibitors/cytokines might prove effective for the treatment of distant tumors by enhancing antitumor immunity [8]. Notably, a majority of preclinical studies performed using immunodeficient mice, such as athymic nude mice, which prevented the ICD effects and a more robust anti-tumor immune response when combined with immunomodulators. However, in mice with intact immune systems, in addition to the local antitumor immune response, NIR-PIT might induce systemic cytokine storms, which may lead to potential adverse effects [84]. Third, issues have been reported regarding the selection of target molecules, development of tumor lysis syndrome, and the high-cost of TSPCs. However, potential solutions are being investigated, and new strategies and techniques are being developed [22, 23].


NIR-PIT has been widely studied in various types of cancer, and noncancerous diseases, both in vitro and in vivo animal models, showing promising therapeutic effects and leading to its application in clinical trials. A wide range of targets can be selected for binding by NIR-PIT agents. Often, tumor-associated cell-surface antigens or glycoproteins, such as EGFR, HER2, or PSMA, are used as tumor-homing targets, given that they are overexpressed on many tumors. In the TME, targets expressed on the non-tumoral cells that support and maintain the tumor have also been investigated. Moreover, multiple antigens can be targeted simultaneously using a cocktail of TSPCs in an NIR-PIT setting.

Antigen-specific recognition agents, including antibodies and their fragments, peptides, protein ligands, and small molecules have been employed in the construction of TSPCs. Each has different advantages and can be selected depending on the function and cellular distribution of the antigen.

NIR light is also a critical factor in NIR-PIT. Light dose, power density, and source influence the therapeutic efficacy. Light delivery systems are of great importance for NIR-PIT. Although NIR light is most commonly delivered by an external light source, endoscopy and wireless implantable light systems have also been used. NIR-PIT, as an adjunctive therapy, could eliminate residual tumor cells after BLS, which is also feasible to reduce metastasis and local recurrence. NIR-PIT can be combined with other modalities to achieve improved antitumor effects. The advantages of these combinations can be ascribed to SUPR effects and other synergistic antitumor mechanisms, such as enhancing antitumor immunity by combination with an immune checkpoint blockade. Recent studies have reported only minimal adverse events associated with NIR-PIT. However, the safe range of TSPC doses and light doses should be further explored. Overall, NIR-PIT holds great promise for clinical applications to treat cancer and other non-malignant diseases with targetable antigens/receptors. However, the toxicity and safety of NIR-PIT must be fully investigated before it can be translated into clinical settings.


ADC: antibody-drug conjugate; ADCC: antibody-dependent cell-mediated cytotoxicity; aPDT: antimicrobial photodynamic therapy; BL: B cell lymphoma; BLS: bright light surgery; CAF: cancer-associated fibroblast; CCA: cholangiocarcinoma; CEA: carcinoembryonic antigen; CR: complete remission; CRC: colorectal cancer; CSC: cancer stem cell; CSCC: cutaneous squamous cell carcinoma; DAMP: damage-associated molecular pattern; EC: esophageal cancer; ECM: extracellular matrix; FPLCA: familial primary localized cutaneous amyloidosis; GBM: glioblastoma; GIST: gastrointestinal stromal tumor; GPCR: G protein-coupled receptors; GSA: galactosyl serum albumin; HCC: hepatocellular carcinoma; HNSCC: head and neck squamous cell carcinoma; ICD: immunogenic cell death; MDR: multidrug resistant; MDSC: myeloid-derived suppressor cell; MF: mycosis fungoides; MPM: malignant pleural mesothelioma; NHS: N-hydroxysuccinimide ester; NIR-PIT: near-infrared photoimmunotherapy; PC: pancreatic carcinoma; PCa: prostate cancer; PDOX: patient-derived orthotopic xenograft; PDT: photodynamic therapy; PS: photosensitizer; RA: rheumatoid arthritis; SCCHN: squamous cell carcinoma of the head and neck; SCLC: small cell lung cancer; SF: synoviocyte; SSc: systemic sclerosis; SUPR: superenhanced permeability and retention; TAA: tumor-associated antigen; TKI: tyrosine kinase inhibitor; TME: tumor microenvironment; TNBC: triple-negative breast cancer; TSPC: target-specific photosensitizer conjugate; VLP: virus-like particle.


This work was supported by China Postdoctoral Science Foundation (grant number 2020M673257); the 1.3.5 project for disciplines of excellence, West China Hospital, Sichuan University (grant number ZYJC21036); the Science and Technology Department of Sichuan Province (grant number 2022NSFSC1414); and the National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University (grant number Z20201014).

Competing Interests

MRH declares the following potential conflicts of interest. Scientific Advisory Boards: Transdermal Cap Inc, Cleveland, OH; Hologenix Inc. Santa Monica, CA; Vielight, Toronto, Canada; JOOVV Inc, Minneapolis-St. Paul MN; Consulting; USHIO Corp, Japan; Sanofi-Aventis Deutschland GmbH, Frankfurt am Main, Germany. Stockholding: Niraxx Light Therapeutics, Inc, Irvine CA; JelikaLite Corp, New York NY. The other authors declare no competing interests.


1. Hu T, Wang Z, Shen W, Liang R, Yan D, Wei M. Recent advances in innovative strategies for enhanced cancer photodynamic therapy. Theranostics. 2021;11:3278-300

2. Mitsunaga M, Ogawa M, Kosaka N, Rosenblum LT, Choyke PL, Kobayashi H. Cancer cell-selective in vivo near infrared photoimmunotherapy targeting specific membrane molecules. Nat Med. 2011;17:1685-91

3. Kobayashi H, Choyke PL. Near-infrared photoimmunotherapy of cancer. Acc Chem Res. 2019;52:2332-9

4. Sato K, Ando K, Okuyama S, Moriguchi S, Ogura T, Totoki S. et al. Photoinduced ligand release from a silicon phthalocyanine dye conjugated with monoclonal antibodies: A mechanism of cancer cell cytotoxicity after near-infrared photoimmunotherapy. ACS Cent Sci. 2018;4:1559-69

5. Kato T, Okada R, Goto Y, Furusawa A, Inagaki F, Wakiyama H. et al. Electron donors rather than reactive oxygen species needed for therapeutic photochemical reaction of near-infrared photoimmunotherapy. ACS Pharmacol Transl Sci. 2021;4:1689-701

6. Ogawa M, Takakura H. Photoimmunotherapy: A new cancer treatment using photochemical reactions. Bioorg Med Chem. 2021;43:116274

7. Ogawa M, Tomita Y, Nakamura Y, Lee MJ, Lee S, Tomita S. et al. Immunogenic cancer cell death selectively induced by near infrared photoimmunotherapy initiates host tumor immunity. Oncotarget. 2017;8:10425-36

8. Kobayashi H, Furusawa A, Rosenberg A, Choyke PL. Near-infrared photoimmunotherapy of cancer: a new approach that kills cancer cells and enhances anti-cancer host immunity. Int Immunol. 2021;33:7-15

9. Maruoka Y, Wakiyama H, Choyke PL, Kobayashi H. Near infrared photoimmunotherapy for cancers: A translational perspective. EBioMedicine. 2021;70:103501

10. Nakajima K, Takakura H, Shimizu Y, Ogawa M. Changes in plasma membrane damage inducing cell death after treatment with near-infrared photoimmunotherapy. Cancer Sci. 2018;109:2889-96

11. Rosenkranz AA, Jans DA, Sobolev AS. Targeted intracellular delivery of photosensitizers to enhance photodynamic efficiency. Immunol Cell Biol. 2000;78:452-64

12. Shirasu N, Yamada H, Shibaguchi H, Kuroki M, Kuroki M. Potent and specific antitumor effect of CEA-targeted photoimmunotherapy. Int J Cancer. 2014;135:2697-710

13. Lo PC, Rodriguez-Morgade MS, Pandey RK, Ng DKP, Torres T, Dumoulin F. The unique features and promises of phthalocyanines as advanced photosensitisers for photodynamic therapy of cancer. Chem Soc Rev. 2020;49:1041-56

14. Peng X, Draney DR, Volcheck WM, Bashford GR, Lamb DT, Grone DL. et al. Phthalocyanine dye as an extremely photostable and highly fluorescent near-infrared labeling reagent. In: Achilefu SI, Bornhop DJ, Raghavachari R, Eds. Proc. SPIE 6097, Optical Molecular Probes for Biomedical Applications. 2006:113-24

15. Inagaki FF, Fujimura D, Furusawa A, Okada R, Wakiyama H, Kato T. et al. Diagnostic imaging in near-infrared photoimmunotherapy using a commercially available camera for indocyanine green. Cancer Sci. 2021;112:1326-30

16. Okuyama S, Fujimura D, Inagaki F, Okada R, Maruoka Y, Wakiyama H. et al. Real-time IR700 fluorescence imaging during near-infrared photoimmunotherapy using a clinically-approved camera for indocyanine green. Cancer Diagn Progn. 2021;1:29-34

17. Sano K, Nakajima T, Choyke PL, Kobayashi H. Markedly enhanced permeability and retention effects induced by photo-immunotherapy of tumors. ACS Nano. 2013;7:717-24

18. Sano K, Nakajima T, Choyke PL, Kobayashi H. The effect of photoimmunotherapy followed by liposomal daunorubicin in a mixed tumor model: a demonstration of the super-enhanced permeability and retention effect after photoimmunotherapy. Mol Cancer Ther. 2014;13:426-32

19. Mitsunaga M, Nakajima T, Sano K, Choyke PL, Kobayashi H. Near-infrared theranostic photoimmunotherapy (PIT): repeated exposure of light enhances the effect of immunoconjugate. Bioconjug Chem. 2012;23:604-9

20. Nagaya T, Nakamura Y, Sato K, Harada T, Choyke PL, Kobayashi H. Improved micro-distribution of antibody-photon absorber conjugates after initial near infrared photoimmunotherapy (NIR-PIT). J Control Release. 2016;232:1-8

21. Ogata F, Nagaya T, Nakamura Y, Sato K, Okuyama S, Maruoka Y. et al. Near-infrared photoimmunotherapy: a comparison of light dosing schedules. Oncotarget. 2017;8:35069-75

22. Paraboschi I, Turnock S, Kramer-Marek G, Musleh L, Barisa M, Anderson J. et al. Near-infrared photoimmunotherapy (NIR-PIT) for the local control of solid cancers: challenges and potentials for human applications. Crit Rev Oncol Hematol. 2021;161:103325

23. Matsuoka K, Sato M, Sato K. Hurdles for the wide implementation of photoimmunotherapy. Immunotherapy. 2021;13:1427-38

24. Kobayashi H, Choyke PL. Super enhanced permeability and retention (SUPR) effects in tumors following near infrared photoimmunotherapy. Nanoscale. 2016;8:12504-9

25. Kato T, Wakiyama H, Furusawa A, Choyke PL, Kobayashi H. Near infrared photoimmunotherapy; A review of targets for cancer therapy. Cancers (Basel). 2021;13:2535

26. Juweid M, Neumann R, Paik C, Perez-Bacete MJ, Sato J, van Osdol W. et al. Micropharmacology of monoclonal antibodies in solid tumors: direct experimental evidence for a binding site barrier. Cancer Res. 1992;52:5144-53

27. Nakajima T, Sano K, Choyke PL, Kobayashi H. Improving the efficacy of photoimmunotherapy (PIT) using a cocktail of antibody conjugates in a multiple antigen tumor model. Theranostics. 2013;3:357-65

28. Chaux A, Cohen JS, Schultz L, Albadine R, Jadallah S, Murphy KM. et al. High epidermal growth factor receptor immunohistochemical expression in urothelial carcinoma of the bladder is not associated with EGFR mutations in exons 19 and 21: A study using formalin-fixed, paraffin-embedded archival tissues. Hum Pathol. 2012;43:1590-5

29. Furnari FB, Cloughesy TF, Cavenee WK, Mischel PS. Heterogeneity of epidermal growth factor receptor signalling networks in glioblastoma. Nat Rev Cancer. 2015;15:302-10

30. Bang YJ, Van Cutsem E, Feyereislova A, Chung HC, Shen L, Sawaki A. et al. Trastuzumab in combination with chemotherapy versus chemotherapy alone for treatment of HER2-positive advanced gastric or gastro-oesophageal junction cancer (ToGA): A phase 3, open-label, randomised controlled trial. Lancet. 2010;376:687-97

31. Park DI, Yun JW, Park JH, Oh SJ, Kim HJ, Cho YK. et al. HER-2/neu amplification is an independent prognostic factor in gastric cancer. Dig Dis Sci. 2006;51:1371-9

32. Grandis JR, Tweardy DJ. Elevated levels of transforming growth factor α and epidermal growth factor receptor messenger RNA are early markers of carcinogenesis in head and neck cancer. Cancer Res. 1993;53:3579-84

33. Burley TA, Mączyńska J, Shah A, Szopa W, Harrington KJ, Boult JKR. et al. Near-infrared photoimmunotherapy targeting EGFR-Shedding new light on glioblastoma treatment. Int J Cancer. 2018;142:2363-74

34. Moore LS, de Boer E, Warram JM, Tucker MD, Carroll WR, Korb ML. et al. Photoimmunotherapy of residual disease after incomplete surgical resection in head and neck cancer models. Cancer Med. 2016;5:1526-34

35. Van Driel PBAA, Boonstra MC, Slooter MD, Heukers R, Stammes MA, Snoeks TJA. et al. EGFR targeted nanobody-photosensitizer conjugates for photodynamic therapy in a pre-clinical model of head and neck cancer. J Control Release. 2016;229:93-105

36. Railkar R, Krane LS, Li QQ, Sanford T, Siddiqui MR, Haines D. et al. Epidermal growth factor receptor (EGFR)-targeted photoimmunotherapy (PIT) for the treatment of EGFR-expressing bladder cancer. Mol Cancer Ther. 2017;16:2201-14

37. Siddiqui MR, Railkar RS, Sanford T, Choyke PL, Kobayashi H, Agarwal PK. Targeting epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 2 expressing bladder cancer using combination photoimmunotherapy (PIT). J Clin Oncol. 2017;35:5

38. Nagaya T, Okuyama S, Ogata F, Maruoka Y, Knapp DW, Karagiannis SN. et al. Near infrared photoimmunotherapy targeting bladder cancer with a canine anti-epidermal growth factor receptor (EGFR) antibody. Oncotarget. 2018;9:19026-38

39. Sato K, Nagaya T, Mitsunaga M, Choyke PL, Kobayashi H. Near infrared photoimmunotherapy for lung metastases. Cancer Lett. 2015;365:112-21

40. Nakamura Y, Ohler ZW, Householder D, Nagaya T, Sato K, Okuyama S. et al. Near infrared photoimmunotherapy in a transgenic mouse model of spontaneous epidermal growth factor receptor (EGFR)-expressing lung cancer. Mol Cancer Ther. 2017;16:408-14

41. Sato K, Nagaya T, Nakamura Y, Harada T, Choyke PL, Kobayashi H. Near infrared photoimmunotherapy prevents lung cancer metastases in a murine model. Oncotarget. 2015;6:19747-58

42. Sato K, Nagaya T, Choyke PL, Kobayashi H. Near infrared photoimmunotherapy in the treatment of pleural disseminated NSCLC: preclinical experience. Theranostics. 2015;5:698-709

43. Sato K, Hanaoka H, Watanabe R, Nakajima T, Choyke PL, Kobayashi H. Near infrared photoimmunotherapy in the treatment of disseminated peritoneal ovarian cancer. Mol Cancer Ther. 2015;14:141-50

44. Bauerschlag D, Meinhold-Heerlein I, Maass N, Bleilevens A, Bräutigam K, Al Rawashdeh W. et al. Detection and specific elimination of EGFR+ ovarian cancer cells using a near infrared photoimmunotheranostic approach. Pharm Res. 2017;34:696-703

45. van Lith SAM, van den Brand D, Wallbrecher R, Wübbeke L, van Duijnhoven SMJ, Mäkinen PI. et al. The effect of subcellular localization on the efficiency of EGFR-targeted VHH photosensitizer conjugates. Eur J Pharm Biopharm. 2018;124:63-72

46. Nagaya T, Sato K, Harada T, Nakamura Y, Choyke PL, Kobayashi H. Near infrared photoimmunotherapy targeting EGFR positive triple negative breast cancer: optimizing the conjugate-light regimen. PLoS One. 2015;10:e0136829

47. Nagaya T, Gorka AP, Nani RR, Okuyama S, Ogata F, Maruoka Y. et al. Molecularly targeted cancer combination therapy with near-infrared photoimmunotherapy and near-infrared photorelease with duocarmycin-antibody conjugate. Mol Cancer Ther. 2018;17:661-70

48. Ito K, Mitsunaga M, Nishimura T, Saruta M, Iwamoto T, Kobayashi H. et al. Near-infrared photochemoimmunotherapy by photoactivatable bifunctional antibody-drug conjugates targeting human epidermal growth factor receptor 2 positive cancer. Bioconjug Chem. 2017;28:1458-69

49. Amoury M, Bauerschlag D, Zeppernick F, von Felbert V, Berges N, Di Fiore S. et al. Photoimmunotheranostic agents for triple-negative breast cancer diagnosis and therapy that can be activated on demand. Oncotarget. 2016;7:54925-36

50. Mitsunaga M, Nakajima T, Sano K, Kramer-Marek G, Choyke PL, Kobayashi H. Immediate in vivo target-specific cancer cell death after near infrared photoimmunotherapy. BMC Cancer. 2012;12:345

51. Hartmans E, Linssen MD, Sikkens C, Levens A, Witjes MJH, van Dam GM. et al. Tyrosine kinase inhibitor induced growth factor receptor upregulation enhances the efficacy of near-infrared targeted photodynamic therapy in esophageal adenocarcinoma cell lines. Oncotarget. 2017;8:29846-56

52. Ito K, Mitsunaga M, Arihiro S, Saruta M, Matsuoka M, Kobayashi H. et al. Molecular targeted photoimmunotherapy for HER2-positive human gastric cancer in combination with chemotherapy results in improved treatment outcomes through different cytotoxic mechanisms. BMC Cancer. 2016;16:37

53. Ito K, Mitsunaga M, Nishimura T, Kobayashi H, Tajiri H. Combination photoimmunotherapy with monoclonal antibodies recognizing different epitopes of human epidermal growth factor receptor 2: an assessment of phototherapeutic effect based on fluorescence molecular imaging. Oncotarget. 2016;7:14143-52

54. Nagaya T, Okuyama S, Ogata F, Maruoka Y, Choyke PL, Kobayashi H. Endoscopic near infrared photoimmunotherapy using a fiber optic diffuser for peritoneal dissemination of gastric cancer. Cancer Sci. 2018;109:1902-8

55. Nagaya T, Okuyama S, Ogata F, Maruoka Y, Choyke PL, Kobayashi H. Near infrared photoimmunotherapy using a fiber optic diffuser for treating peritoneal gastric cancer dissemination. Gastric Cancer. 2019;22:463-72

56. Nishimura T, Mitsunaga M, Ito K, Kobayashi H, Saruta M. Cancer neovasculature-targeted near-infrared photoimmunotherapy (NIR-PIT) for gastric cancer: different mechanisms of phototoxicity compared to cell membrane-targeted NIR-PIT. Gastric Cancer. 2020;23:82-94

57. Maawy AA, Hiroshima Y, Zhang Y, Garcia-Guzman M, Luiken GA, Kobayashi H. et al. Photoimmunotherapy lowers recurrence after pancreatic cancer surgery in orthotopic nude mouse models. J Surg Res. 2015;197:5-11

58. Hollandsworth HM, Amirfakhri S, Filemoni F, Molnar J, Hoffman RM, Yazaki P. et al. Near-infrared photoimmunotherapy is effective treatment for colorectal cancer in orthotopic nude-mouse models. PLoS One. 2020;15:e0234643

59. Hanaoka H, Nagaya T, Sato K, Nakamura Y, Watanabe R, Harada T. et al. Glypican-3 targeted human heavy chain antibody as a drug carrier for hepatocellular carcinoma therapy. Mol Pharm. 2015;12:2151-7

60. Hanaoka H, Nakajima T, Sato K, Watanabe R, Phung Y, Gao W. et al. Photoimmunotherapy of hepatocellular carcinoma-targeting Glypican-3 combined with nanosized albumin-bound paclitaxel. Nanomedicine (Lond). 2015;10:1139-47

61. Chen Y, Chatterjee S, Lisok A, Minn I, Pullambhatla M, Wharram B. et al. A PSMA-targeted theranostic agent for photodynamic therapy. J Photochem Photobiol B. 2017;167:111-6

62. Nagaya T, Nakamura Y, Okuyama S, Ogata F, Maruoka Y, Choyke PL. et al. Near-infrared photoimmunotherapy targeting prostate cancer with prostate-specific membrane antigen (PSMA) antibody. Mol Cancer Res. 2017;15:1153-62

63. Lütje S, Heskamp S, Franssen GM, Frielink C, Kip A, Hekman M. et al. Development and characterization of a theranostic multimodal anti-PSMA targeting agent for imaging, surgical guidance, and targeted photodynamic therapy of PSMA-expressing tumors. Theranostics. 2019;9:2924-38

64. Wang X, Tsui B, Ramamurthy G, Zhang P, Meyers J, Kenney ME. et al. Theranostic agents for photodynamic therapy of prostate cancer by targeting prostate-specific membrane antigen. Mol Cancer Ther. 2016;15:1834-44

65. Nagaya T, Nakamura Y, Sato K, Harada T, Choyke PL, Kobayashi H. Near infrared photoimmunotherapy of B-cell lymphoma. Mol Oncol. 2016;10:1404-14

66. Heryanto YD, Hanaoka H, Nakajima T, Yamaguchi A, Tsushima Y. Applying near-infrared photoimmunotherapy to B-cell lymphoma: comparative evaluation with radioimmunotherapy in tumor xenografts. Ann Nucl Med. 2017;31:669-77

67. Wei W, Jiang D, Ehlerding EB, Barnhart TE, Yang Y, Engle JW. et al. CD146-targeted multimodal image-guided photoimmunotherapy of melanoma. Adv Sci (Weinh). 2019;6:1801237

68. Li W, Zhang H, Assaraf YG, Zhao K, Xu X, Xie J. et al. Overcoming ABC transporter-mediated multidrug resistance: molecular mechanisms and novel therapeutic drug strategies. Drug Resist Updat. 2016;27:14-29

69. Mao C, Zhao Y, Li F, Li Z, Tian S, Debinski W. et al. P-glycoprotein targeted and near-infrared light-guided depletion of chemoresistant tumors. J Control Release. 2018;286:289-300

70. Mao C, Qu P, Miley MJ, Zhao Y, Li Z, Ming X. P-glycoprotein targeted photodynamic therapy of chemoresistant tumors using recombinant Fab fragment conjugates. Biomater Sci. 2018;6:3063-74

71. Mao CQ, Li F, Zhao Y, Debinski W, Ming X. P-glycoprotein-targeted photodynamic therapy boosts cancer nanomedicine by priming tumor microenvironment. Theranostics. 2018;8:6274-90

72. Jin J, Krishnamachary B, Mironchik Y, Kobayashi H, Bhujwalla ZM. Phototheranostics of CD44-positive cell populations in triple negative breast cancer. Sci Rep. 2016;6:27871

73. Nagaya T, Nakamura Y, Okuyama S, Ogata F, Maruoka Y, Choyke PL. et al. Syngeneic mouse models of oral cancer are effectively targeted by anti-CD44-Based NIR-PIT. Mol Cancer Res. 2017;15:1667-77

74. Jing H, Weidensteiner C, Reichardt W, Gaedicke S, Zhu X, Grosu AL. et al. Imaging and selective elimination of glioblastoma stem cells with theranostic near-infrared-labeled CD133-specific antibodies. Theranostics. 2016;6:862-74

75. Kiss B, van den Berg NS, Ertsey R, McKenna K, Mach KE, Zhang CA. et al. CD47-targeted near-infrared photoimmunotherapy for human bladder cancer. Clin Cancer Res. 2019;25:3561-71

76. Watanabe S, Noma K, Ohara T, Kashima H, Sato H, Kato T. et al. Photoimmunotherapy for cancer-associated fibroblasts targeting fibroblast activation protein in human esophageal squamous cell carcinoma. Cancer Biol Ther. 2019;20:1234-48

77. Xue G, Wang Z, Zheng N, Fang J, Mao C, Li X. et al. Elimination of acquired resistance to PD-1 blockade via the concurrent depletion of tumour cells and immunosuppressive cells. Nat Biomed Eng. 2021;5:1306-19

78. Shi Q, Tao Z, Yang H, Fan Q, Wei D, Wan L. et al. PDGFRbeta-specific Affibody-directed delivery of a photosensitizer, IR700, is efficient for vascular-targeted photodynamic therapy of colorectal cancer. Drug Deliv. 2017;24:1818-30

79. Li F, Zhao Y, Mao C, Kong Y, Ming X. RGD-Modified albumin nanoconjugates for targeted delivery of a porphyrin photosensitizer. Mol Pharm. 2017;14:2793-804

80. Dou XB, Nomoto T, Takemoto H, Matsui M, Tomoda K, Nishiyama N. Effect of multiple cyclic RGD peptides on tumor accumulation and intratumoral distribution of IRDye 700DX-conjugated polymers. Sci Rep. 2018;8:8126

81. Zhao Y, Li F, Mao CQ, Ming X. Multiarm nanoconjugates for cancer cell-targeted delivery of photosensitizers. Mol Pharm. 2018;15:2559-69

82. Okada R, Maruoka Y, Furusawa A, Inagaki F, Nagaya T, Fujimura D. et al. The Effect of antibody fragments on CD25 targeted regulatory T cell near-infrared photoimmunotherapy. Bioconjug Chem. 2019;30:2624-33

83. Maruoka Y, Furusawa A, Okada R, Inagaki F, Fujimura D, Wakiyama H. et al. Combined CD44- and CD25-targeted near-infrared photoimmunotherapy selectively kills cancer and regulatory T cells in syngeneic mouse cancer models. Cancer Immunol Res. 2020;8:345-55

84. Sato K, Sato N, Xu BY, Nakamura Y, Nagaya T, Choyke PL. et al. Spatially selective depletion of tumor-associated regulatory T cells with near-infrared photoimmunotherapy. Sci Transl Med. 2016;8:352ra110

85. Wakiyama H, Furusawa A, Okada R, Inagaki F, Kato T, Furumoto H. et al. Opening up new VISTAs: V-domain immunoglobulin suppressor of T cell activation (VISTA) targeted near-infrared photoimmunotherapy (NIR-PIT) for enhancing host immunity against cancers. Cancer Immunol Immunother. 2022 doi: 10.1007/s00262-022-03205-5

86. Siddiqui MR, Railkar R, Sanford T, Crooks DR, Eckhaus MA, Haines D. et al. Targeting epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 2 (HER2) expressing bladder cancer using combination photoimmunotherapy (PIT). Sci Rep. 2019;9:2084

87. Mashayekhi V, Xenaki KT, van Bergen En Henegouwen PMP, Oliveira S. Dual Targeting of endothelial and cancer cells potentiates in vitro nanobody-targeted photodynamic therapy. Cancers (Basel). 2020;12:2732

88. Deken MM, Kijanka MM, Beltrán Hernández I, Slooter MD, de Bruijn HS, van Diest PJ. et al. Nanobody-targeted photodynamic therapy induces significant tumor regression of trastuzumab-resistant HER2-positive breast cancer, after a single treatment session. J Control Release. 2020;323:269-81

89. Watanabe R, Hanaoka H, Sato K, Nagaya T, Harada T, Mitsunaga M. et al. Photoimmunotherapy targeting prostate-specific membrane antigen: are antibody fragments as effective as antibodies?. J Nucl Med. 2015;56:140-4

90. Bao R, Wang YP, Lai JH, Zhu H, Zhao Y, Li SP. et al. Enhancing anti-PD-1/PD-L1 immune checkpoint inhibitory cancer therapy by CD276-targeted photodynamic ablation of tumor cells and tumor vasculature. Mol Pharm. 2019;16:339-48

91. Heukers R, Mashayekhi V, Ramirez-Escudero M, de Haard H, Verrips TC, van Bergen En Henegouwen PMP. et al. VHH-photosensitizer conjugates for targeted photodynamic therapy of met-overexpressing tumor cells. Antibodies (Basel). 2019;8:26

92. De Groof TWM, Mashayekhi V, Fan TS, Bergkamp ND, Sastre Toraño J, van Senten JR. et al. Nanobody-targeted photodynamic therapy selectively kills viral GPCR-expressing glioblastoma cells. Mol Pharm. 2019;16:3145-56

93. Mączyńska J, Da Pieve C, Burley TA, Raes F, Shah A, Saczko J. et al. Immunomodulatory activity of IR700-labelled Affibody targeting HER2. Cell Death Dis. 2020;11:886

94. Derks YHW, Rijpkema M, Amatdjais-Groenen HIV, Kip A, Franssen GM, Sedelaar JPM. et al. Photosensitizer-based multimodal PSMA-targeting ligands for intraoperative detection of prostate cancer. Theranostics. 2021;11:1527-541

95. Kines RC, Varsavsky I, Choudhary S, Bhattacharya D, Spring S, McLaughlin R. et al. An infrared dye-conjugated virus-like particle for the treatment of primary uveal melanoma. Mol Cancer Ther. 2018;17:565-74

96. Shirasu N, Shibaguchi H, Yamada H, Kuroki M, Yasunaga S. Highly versatile cancer photoimmunotherapy using photosensitizer-conjugated avidin and biotin-conjugated targeting antibodies. Cancer Cell Int. 2019;19:299

97. Sato K, Watanabe R, Hanaoka H, Harada T, Nakajima T, Kim I. et al. Photoimmunotherapy: comparative effectiveness of two monoclonal antibodies targeting the epidermal growth factor receptor. Mol Oncol. 2014;8:620-32

98. Fujimura D, Inagaki F, Okada R, Rosenberg A, Furusawa A, Choyke PL. et al. Conjugation ratio, light dose, and pH affect the stability of panitumumab-IR700 for near-infrared photoimmunotherapy. ACS Med Chem Lett. 2020;11:1598-604

99. Nakajima K, Kimura T, Takakura H, Yoshikawa Y, Kameda A, Shindo T. et al. Implantable wireless powered light emitting diode (LED) for near-infrared photoimmunotherapy: device development and experimental assessment in vitro and in vivo. Oncotarget. 2018;9:20048-57

100. Nakajima T, Sato K, Hanaoka H, Watanabe R, Harada T, Choyke PL. et al. The effects of conjugate and light dose on photo-immunotherapy induced cytotoxicity. BMC Cancer. 2014;14:389

101. Sato K, Watanabe R, Hanaoka H, Nakajima T, Choyke PL, Kobayashi H. Comparative effectiveness of light emitting diodes (LEDs) and Lasers in near infrared photoimmunotherapy. Oncotarget. 2016;7:14324-35

102. Nagaya T, Friedman J, Maruoka Y, Ogata F, Okuyama S, Clavijo PE. et al. Host immunity following near-infrared photoimmunotherapy is enhanced with PD-1 checkpoint blockade to eradicate established antigenic tumors. Cancer Immunol Res. 2019;7:401-13

103. Maruoka Y, Furusawa A, Okada R, Inagaki F, Fujimura D, Wakiyama H. et al. Near-infrared photoimmunotherapy combined with ctla4 checkpoint blockade in syngeneic mouse cancer models. Vaccines (Basel). 2020;8:528

104. Maruoka Y, Furusawa A, Okada R, Inagaki F, Wakiyama H, Kato T. et al. Interleukin-15 after near-infrared photoimmunotherapy (NIR-PIT) enhances T cell response against syngeneic mouse tumors. Cancers (Basel). 2020;12:2575

105. Tedder TF, Engel P. CD20: a regulator of cell-cycle progression of B lymphocytes. Immunol Today. 1994;15:450-4

106. Read ED, Eu P, Little PJ, Piva TJ. The status of radioimmunotherapy in CD20+ non-Hodgkin's lymphoma. Target Oncol. 2015;10:15-26

107. Fulkerson CM, Dhawan D, Ratliff TL, Hahn NM, Knapp DW. Naturally occurring canine invasive urinary bladder cancer: A complementary animal model to improve the success rate in human clinical trials of new cancer drugs. Int J Genomics. 2017;2017:6589529

108. Røtterud R, Nesland JM, Berner A, Fosså SD. Expression of the epidermal growth factor receptor family in normal and malignant urothelium. BJU Int. 2005;95:1344-50

109. Cognetti DM, Johnson JM, Curry JM, Kochuparambil ST, McDonald D, Mott F. et al. Phase 1/2a, open-label, multicenter study of RM-1929 photoimmunotherapy in patients with locoregional, recurrent head and neck squamous cell carcinoma. Head Neck. 2021;43:3875-87

110. Tahara M, Okano S, Enokida T, Ueda Y, Fujisawa T, Shinozaki T. et al. A phase I, single-center, open-label study of RM-1929 photoimmunotherapy in Japanese patients with recurrent head and neck squamous cell carcinoma. Int J Clin Oncol. 2021;26:1812-21

111. Barbar T, Jaffer Sathick I. Tumor lysis syndrome. Adv Chronic Kidney Dis. 2021;28:438-46.e1

112. Pan Y, Volkmer JP, Mach KE, Rouse RV, Liu JJ, Sahoo D. et al. Endoscopic molecular imaging of human bladder cancer using a CD47 antibody. Sci Transl Med. 2014;6:260ra148

113. Chao MP, Jaiswal S, Weissman-Tsukamoto R, Alizadeh AA, Gentles AJ, Volkmer J. et al. Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47. Sci Transl Med. 2010;2:63ra94

114. Nakamura YA, Okuyama S, Furusawa A, Nagaya T, Fujimura D, Okada R. et al. Near-infrared photoimmunotherapy through bone. Cancer Sci. 2019;110:3689-94

115. Aerssens J, Boonen S, Lowet G, Dequeker J. Interspecies differences in bone composition, density, and quality: potential implications for in vivo bone research. Endocrinology. 1998;139:663-70

116. Soroceanu L, Matlaf L, Bezrookove V, Harkins L, Martinez R, Greene M. et al. Human cytomegalovirus US28 found in glioblastoma promotes an invasive and angiogenic phenotype. Cancer Res. 2011;71:6643-53

117. Sexton M, Woodruff G, Horne EA, Lin YH, Muccioli GG, Bai M. et al. NIR-mbc94, a fluorescent ligand that binds to endogenous CB (2) receptors and is amenable to high-throughput screening. Chem Biol. 2011;18:563-8

118. Jia N, Zhang S, Shao P, Bagia C, Janjic JM, Ding Y. et al. Cannabinoid CB2 receptor as a new phototherapy target for the inhibition of tumor growth. Mol Pharm. 2014;11:1919-29

119. Zhang SJ, Jia NY, Shao P, Tong Q, Xie XQ, Bai MF. Target-selective phototherapy using a ligand-based photosensitizer for type 2 cannabinoid receptor. Chem Biol. 2014;21:338-44

120. Hermann PC, Huber SL, Herrler T, Aicher A, Ellwart JW, Guba M. et al. Distinct populations of cancer stem cells determine tumor growth and metastatic activity in human pancreatic cancer. Cell Stem Cell. 2007;1:313-23

121. Liu S, Cong Y, Wang D, Sun Y, Deng L, Liu Y. et al. Breast cancer stem cells transition between epithelial and mesenchymal states reflective of their normal counterparts. Stem Cell Rep. 2014;2:78-91

122. Singh SK, Hawkins C, Clarke ID, Squire JA, Bayani J, Hide T. et al. Identification of human brain tumour initiating cells. Nature. 2004;432:396-401

123. Ross JS, Slodkowska EA, Symmans WF, Pusztai L, Ravdin PM, Hortobagyi GN. The HER-2 receptor and breast cancer: ten years of targeted anti-HER-2 therapy and personalized medicine. Oncologist. 2009;14:320-68

124. Yamaguchi H, Pantarat N, Suzuki T, Evdokiou A. Near-infrared photoimmunotherapy using a small protein mimetic for HER2-overexpressing breast cancer. Int J Mol Sci. 2019;20:5835

125. Yamaguchi H, On J, Morita T, Suzuki T, Okada Y, Ono J. et al. Combination of near-infrared photoimmunotherapy using trastuzumab and small protein mimetic for HER2-positive breast cancer. Int J Mol Sci. 2021;22:12213

126. Nani RR, Gorka AP, Nagaya T, Yamamoto T, Ivanic J, Kobayashi H. et al. In vivo activation of duocarmycin-antibody conjugates by near-infrared light. ACS Cent Sci. 2017;3:329-37

127. Bertucci F, Finetti P, Birnbaum D. Basal breast cancer: a complex and deadly molecular subtype. Curr Mol Med. 2012;12:96-110

128. Wang X, Osada T, Wang Y, Yu L, Sakakura K, Katayama A. et al. CSPG4 protein as a new target for the antibody-based immunotherapy of triple-negative breast cancer. J Natl Cancer Inst. 2010;102:1496-512

129. Stratford AL, Reipas K, Maxwell C, Dunn SE. Targeting tumour-initiating cells to improve the cure rates for triple-negative breast cancer. Expert Rev Mol Med. 2010;12:e22

130. Polikarpov DM, Campbell DH, Lund ME, Lu Y, Lu Y, Wu J. et al. The feasibility of Miltuximab®-IRDye700DX-mediated photoimmunotherapy of solid tumors. Photodiagn Photodyn Ther. 2020;32:102064

131. Al-Hajj M, Wicha MS, Benito-Hernandez A, Morrison SJ, Clarke MF. Prospective identification of tumorigenic breast cancer cells. Proc Natl Acad Sci U S A. 2003;100:3983-8

132. Paulis YW, Huijbers EJ, van der Schaft DW, Soetekouw PM, Pauwels P, Tjan-Heijnen VC. et al. CD44 enhances tumor aggressiveness by promoting tumor cell plasticity. Oncotarget. 2015;6:19634-46

133. Krishnamachary B, Penet MF, Nimmagadda S, Mironchik Y, Raman V, Solaiyappan M. et al. Hypoxia regulates CD44 and its variant isoforms through HIF-1alpha in triple negative breast cancer. PLoS One. 2012;7:e44078

134. Nishimura T, Mitsunaga M, Sawada R, Saruta M, Kobayashi H, Matsumoto N. et al. Photoimmunotherapy targeting biliary-pancreatic cancer with humanized anti-TROP2 antibody. Cancer Med. 2019;8:7781-92

135. Hirata H, Kuwatani M, Nakajima K, Kodama Y, Yoshikawa Y, Ogawa M. et al. Near-infrared photoimmunotherapy (NIR-PIT) on cholangiocarcinoma using a novel catheter device with light emitting diodes. Cancer Sci. 2021;112:828-38

136. Elekonawo FMK, Bos DL, Goldenberg DM, Boerman OC, Rijpkema M. Carcinoembryonic antigen-targeted photodynamic therapy in colorectal cancer models. EJNMMI Res. 2019;9:108

137. Wong NACS, Adamczyk LA, Evans S, Cullen J, Oniscu A, Oien KA. A33 shows similar sensitivity to but is more specific than CDX2 as an immunomarker of colorectal carcinoma. Histopathology. 2017;71:34-41

138. Wei DF, Tao Z, Shi QX, Wang LJ, Liu L, She TS. et al. Selective photokilling of colorectal tumors by near-infrared photoimmunotherapy with a GPA33-targeted single-chain antibody variable fragment conjugate. Mol Pharm. 2020;17:2508-17

139. Paulsson J, Sjöblom T, Micke P, Pontén F, Landberg G, Heldin CH. et al. Prognostic significance of stromal platelet-derived growth factor β-receptor expression in human breast cancer. Am J Clin Pathol. 2009;175:334-41

140. Geevarghese A, Herman IM. Pericyte-endothelial crosstalk: implications and opportunities for advanced cellular therapies. Transl Res. 2014;163:296-306

141. She T, Shi Q, Li Z, Feng Y, Yang H, Tao Z. et al. Combination of long-acting TRAIL and tumor cell-targeted photodynamic therapy as a novel strategy to overcome chemotherapeutic multidrug resistance and TRAIL resistance of colorectal cancer. Theranostics. 2021;11:4281-97

142. Nagaya T, Nakamura Y, Sato K, Zhang YF, Ni M, Choyke PL. et al. Near infrared photoimmunotherapy with an anti-mesothelin antibody. Oncotarget. 2016;7:23361-9

143. Loeffler M, Krüger JA, Niethammer AG, Reisfeld RA. Targeting tumor-associated fibroblasts improves cancer chemotherapy by increasing intratumoral drug uptake. J Clin Invest. 2006;116:1955-62

144. Murata T, Mekada E, Hoffman RM. Reconstitution of a metastatic-resistant tumor microenvironment with cancer-associated fibroblasts enables metastasis. Cell Cycle. 2017;16:533-5

145. Borriello L, Nakata R, Sheard MA, Fernandez GE, Sposto R, Malvar J. et al. Cancer-associated fibroblasts share characteristics and protumorigenic activity with mesenchymal stromal cells. Cancer Res. 2017;77:5142-57

146. Katsube R, Noma K, Ohara T, Nishiwaki N, Kobayashi T, Komoto S. et al. Fibroblast activation protein targeted near infrared photoimmunotherapy (NIR PIT) overcomes therapeutic resistance in human esophageal cancer. Sci Rep. 2021;11:1693

147. Nishida T, Takahashi T, Nakajima K, Tsujinaka T, Hirota S. KIT and PDGFRA mutations of gastrointestinal stromal tumor. J Clin Oncol. 2009;27:10560

148. Fujimoto S, Muguruma N, Okamoto K, Kurihara T, Sato Y, Miyamoto Y. et al. A novel theranostic combination of near-infrared fluorescence imaging and laser irradiation targeting c-KIT for gastrointestinal stromal tumors. Theranostics. 2018;8:2313-28

149. McMahon J, O'Brien CJ, Pathak I, Hamill R, McNeil E, Hammersley N. et al. Influence of condition of surgical margins on local recurrence and disease-specific survival in oral and oropharyngeal cancer. Br J Oral Macillofac Surg. 2003;41:224-31

150. Baumhoer D, Tornillo L, Stadlmann S, Roncalli M, Diamantis EK, Terracciano LM. Glypican 3 expression in human nonneoplastic, preneoplastic, and neoplastic tissues: a tissue microarray analysis of 4,387 tissue samples. Am J Clin Pathol. 2008;129:899-906

151. Isobe Y, Sato K, Nishinaga Y, Takahashi K, Taki S, Yasui H. et al. Near infrared photoimmunotherapy targeting DLL3 for small cell lung cancer. EBiomedicine. 2020;52:102632

152. Sharpe AH, Wherry EJ, Ahmed R, Freeman GJ. The function of programmed cell death 1 and its ligands in regulating autoimmunity and infection. Nat Immunol. 2007;8:239-45

153. Wang A, Wang HY, Liu Y, Zhao MC, Zhang HJ, Lu ZY. et al. The prognostic value of PD-L1 expression for non-small cell lung cancer patients: a meta-analysis. Eur J Surg Oncol. 2015;41:450-6

154. Nagaya T, Nakamura Y, Sato K, Harada T, Choyke PL, Hodge JW. et al. Near infrared photoimmunotherapy with avelumab, an anti-programmed death-ligand 1 (PD-L1) antibody. Oncotarget. 2017;8:8807-17

155. Nishinaga Y, Sato K, Yasui H, Taki S, Takahashi K, Shimizu M. et al. Targeted phototherapy for malignant pleural mesothelioma: near-infrared photoimmunotherapy targeting podoplanin. Cells. 2020;9:1019

156. Kato Y, Kaneko MK. A cancer-specific monoclonal antibody recognizes the aberrantly glycosylated podoplanin. Sci Rep. 2014;4:5924

157. Melnikova VO, Balasubramanian K, Villares GJ, Dobroff AS, Zigler M, Wang H. et al. Crosstalk between protease-activated receptor 1 and platelet-activating factor receptor regulates melanoma cell adhesion molecule (MCAM/MUC18) expression and melanoma metastasis. J Biol Chem. 2009;284:28845-55

158. Mallidi S, Anbil S, Bulin AL, Obaid G, Ichikawa M, Hasan T. Beyond the barriers of light penetration: strategies, perspectives and possibilities for photodynamic therapy. Theranostics. 2016;6:2458-87

159. Furusawa A, Okada R, Inagaki F, Wakiyama H, Kato T, Furumoto H. et al. CD29 targeted near-infrared photoimmunotherapy (NIR-PIT) in the treatment of a pigmented melanoma model. Oncoimmunology. 2022;11:2019922

160. Jawed SI, Myskowski PL, Horwitz S, Moskowitz A, Querfeld C. Primary cutaneous T-cell lymphoma (mycosis fungoides and Sézary syndrome): part I. Diagnosis: clinical and histopathologic features and new molecular and biologic markers. J Am Acad Dermatol. 2014;70:205.e1-16

161. Silic-Benussi M, Saponeri A, Michelotto A, Russo I, Colombo A, Pelizzo MG. et al. Near infrared photoimmunotherapy targeting the cutaneous lymphocyte antigen for mycosis fungoides. Expert Opin Biol Ther. 2021;21:977-81

162. Ng JS, Low JJ, Ilancheran A. Epithelial ovarian cancer. Best Pract Res Clin Obstet Gynaecol. 2012;26:337-45

163. Harada T, Nakamura Y, Sato K, Nagaya T, Okuyama S, Ogata F. et al. Near-infrared photoimmunotherapy with galactosyl serum albumin in a model of diffuse peritoneal disseminated ovarian cancer. Oncotarget. 2016;7:79408-16

164. Gunn AJ, Hama Y, Koyama Y, Kohn EC, Choyke PL, Kobayashi H. Targeted optical fluorescence imaging of human ovarian adenocarcinoma using a galactosyl serum albumin-conjugated fluorophore. Cancer Sci. 2007;98:1727-33

165. Carduner L, Picot CR, Leroy-Dudal J, Blay L, Kellouche S, Carreiras F. Cell cycle arrest or survival signaling through αv integrins, activation of PKC and ERK1/2 lead to anoikis resistance of ovarian cancer spheroids. Exp Cell Res. 2014;320:329-42

166. Marelli UK, Rechenmacher F, Sobahi TRA, Mas-Moruno C, Kessler H. Tumor targeting via integrin ligands. Front Oncol. 2013;3:222

167. Gabrilovich DI, Ostrand-Rosenberg S, Bronte V. Coordinated regulation of myeloid cells by tumours. Nat Rev Immunol. 2012;12:253-68

168. Girgis MD, Olafsen T, Kenanova V, McCabe KE, Wu AM, Tomlinson JS. Targeting CEA in pancreas cancer xenografts with a mutated scFv-Fc antibody fragment. EJNMMI Res. 2011;1:24

169. Maawy AA, Hiroshima Y, Zhang Y, Heim R, Makings L, Garcia-Guzman M. et al. Near infra-red photoimmunotherapy with anti-CEA-IR700 results in extensive tumor lysis and a significant decrease in tumor burden in orthotopic mouse models of pancreatic cancer. PloS One. 2015;10:e0121989

170. Hiroshima Y, Maawy A, Zhang Y, Guzman MG, Heim R, Makings L. et al. Photoimmunotherapy inhibits tumor recurrence after surgical resection on a pancreatic cancer patient-derived orthotopic xenograft (PDOX) nude mouse model. Ann Surg Oncol. 2015;22:S1469-74

171. Lum YL, Luk JM, Staunton DE, Ng DKP, Fong WP. Cadherin-17 targeted near-infrared photoimmunotherapy for treatment of gastrointestinal cancer. Mol Pharm. 2020;17:3941-51

172. Gomes-da-Silva LC, Kepp O, Kroemer G. Regulatory approval of photoimmunotherapy: photodynamic therapy that induces immunogenic cell death. Oncoimmunology. 2020;9:1841393

173. Bispo M, Anaya-Sanchez A, Suhani S, Raineri EJM, López-Álvarez M, Heuker M. et al. Fighting Staphylococcus aureus infections with light and photoimmunoconjugates. JCI Insight. 2020;5:e139512

174. Yasui H, Takahashi K, Taki S, Shimizu M, Koike C, Umeda K. et al. Near infrared photo-antimicrobial targeting therapy for Candida albicans. Adv Ther. 2021;4:2000221

175. Reeßing F, Bispo M, López-Álvarez M, van Oosten M, Feringa BL, van Dijl JM. et al. A facile and reproducible synthesis of near-infrared fluorescent conjugates with small targeting molecules for microbial infection imaging. ACS Omega. 2020;5:22071-80

176. Sadraeian M, Bahou C, da Cruz EF, Janini LMR, Sobhie Diaz R, Boyle RW. et al. Photoimmunotherapy using cationic and anionic photosensitizer-antibody conjugates against HIV Env-expressing cells. Int J Mol Sci. 2020;21:9151

177. Dhandapani R, Arokiaraj CM, Taberner FJ, Pacifico P, Raja S, Nocchi L. et al. Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons. Nat Commun. 2018;9:1640

178. Chang DS, Hsu E, Hottinger DG, Cohen SP. Anti-nerve growth factor in pain management: current evidence. J Pain Res. 2016;9:373-83

179. Nocchi L, Portulano C, Franciosa F, Doleschall B, Panea M, Roy N. et al. Nerve growth factor-mediated photoablation of nociceptors reduces pain behavior in mice. Pain. 2019;160:2305-15

180. Cevikbas F, Lerner EA. Physiology and pathophysiology of itch. Physiol Rev. 2020;100:945-82

181. Dillon SR, Sprecher C, Hammond A, Bilsborough J, Rosenfeld-Franklin M, Presnell SR. et al. Interleukin 31, a cytokine produced by activated T cells, induces dermatitis in mice. Nat Immunol. 2004;5:752-60

182. Nocchi L, Roy N, D'Attilia M, Dhandapani R, Maffei M, Traista A. et al. Interleukin-31-mediated photoablation of pruritogenic epidermal neurons reduces itch-associated behaviours in mice. Nat Biomed Eng. 2019;3:114-25

183. Kinova MK. Diagnostics and treatment of insulinoma. Neoplasma. 2015;62:692-704

184. Reubi JC, Waser B. Concomitant expression of several peptide receptors in neuroendocrine tumours: molecular basis for in vivo multireceptor tumour targeting. Eur J Nucl Med Mol Imaging. 2003;30:781-93

185. Boss M, Bos D, Frielink C, Sandker G, Bronkhorst P, van Lith SAM. et al. Receptor-targeted photodynamic therapy of glucagon-like peptide 1 receptor-positive lesions. J Nucl Med. 2020;61:1588-93

186. Huber LC, Distler O, Tarner I, Gay RE, Gay S, Pap T. Synovial fibroblasts: key players in rheumatoid arthritis. Rheumatology (Oxford, U. K.). 2006;45:669-75

187. Laverman P, van der Geest T, Terry SY, Gerrits D, Walgreen B, Helsen MM. et al. Immuno-PET and immuno-SPECT of rheumatoid arthritis with radiolabeled anti-fibroblast activation protein antibody correlates with severity of arthritis. J Nucl Med. 2015;56:778-83

188. Dorst DN, Rijpkema M, Boss M, Walgreen B, Helsen MMA, Bos DL. et al. Targeted photodynamic therapy selectively kills activated fibroblasts in experimental arthritis. Rheumatology (Oxford, U. K.). 2020;59:3952-60

189. Dorst DN, Rijpkema M, Buitinga M, Walgreen B, Helsen MMA, Brennan E. et al. Targeting of fibroblast activation protein in rheumatoid arthritis patients: imaging and ex vivo photodynamic therapy. Rheumatology (Oxford, U. K.). 2022;61:2999-3009

190. van Caam A, Vonk M, van den Hoogen F, van Lent P, van der Kraan P. Unraveling SSc pathophysiology; the myofibroblast. Front Immunol. 2018;9:2452

191. Dorst DN, van Caam APM, Vitters EL, Walgreen B, Helsen MMA, Klein C. et al. Fibroblast activation protein targeted photodynamic therapy selectively kills activated skin fibroblasts from systemic sclerosis patients and prevents tissue contraction. Int J Mol Sci. 2021;22:12681

192. Sato K, Nakajima T, Choyke PL, Kobayashi H. Selective cell elimination in vitro and in vivo from tissues and tumors using antibodies conjugated with a near infrared phthalocyanine. RSC Adv. 2015;5:25105-14

193. Sato K, Choyke PL, Hisataka K. Selective cell elimination from mixed 3D culture using a near infrared photoimmunotherapy technique. J Vis Exp. 2016;109:53633

Author contact

Corresponding address Corresponding authors: Hao Yang, PhD., NHC Key Lab of Transplant Engineering and Immunology, West China Hospital, Sichuan University, No. 1 KeYuan 4th Road, Chengdu, 610041, China. Tel: +86-28-85164031, Fax: +86-28-85164031, Email:; Xian Jiang, PhD., Department of Dermatology, West China Hospital, Sichuan University, No. 37 Guo Xue Alley, Chengdu, 610041, China. Tel: +86-28-85422560, Fax: +86-28-85422560, Email:

Received 2022-5-6
Accepted 2022-9-28
Published 2022-10-9

Citation styles

Wei, D., Qi, J., Hamblin, M.R., Wen, X., Jiang, X., Yang, H. (2022). Near-infrared photoimmunotherapy: design and potential applications for cancer treatment and beyond. Theranostics, 12(16), 7108-7131.

Wei, D.; Qi, J.; Hamblin, M.R.; Wen, X.; Jiang, X.; Yang, H. Near-infrared photoimmunotherapy: design and potential applications for cancer treatment and beyond. Theranostics 2022, 12 (16), 7108-7131. DOI: 10.7150/thno.74820.

Wei D, Qi J, Hamblin MR, Wen X, Jiang X, Yang H. Near-infrared photoimmunotherapy: design and potential applications for cancer treatment and beyond. Theranostics 2022; 12(16):7108-7131. doi:10.7150/thno.74820.

Wei D, Qi J, Hamblin MR, Wen X, Jiang X, Yang H. 2022. Near-infrared photoimmunotherapy: design and potential applications for cancer treatment and beyond. Theranostics. 12(16):7108-7131.

This is an open access article distributed under the terms of the Creative Commons Attribution License ( See for full terms and conditions.
Popup Image