Full Text | PDF

Share on tweeters Share on facebook Share on linkedin Share on googleplus

Theranostics 2023; 13(3):1165-1179. doi:10.7150/thno.81583 This issue Cite

Research Paper

Colonization with two different Blastocystis subtypes in DSS-induced colitis mice is associated with strikingly different microbiome and pathological features

Lei Deng1, Lukasz Wojciech2, Chin Wen Png2, Dorinda Yan Qin Kioh3, Yuxiang Gu3, Thet Tun Aung2, Benoit Malleret2, Eric Chun Yong Chan3, Guangneng Peng4, Yongliang Zhang2, Nicholas Robert John Gascoigne2, Kevin Shyong Wei Tan1✉

1. Laboratory of Molecular and Cellular Parasitology, Department of Microbiology and Immunology, Healthy Longevity Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117545, Singapore
2. Department of Microbiology and Immunology, Immunology Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117545, Singapore
3. Department of Pharmacy, Faculty of Science, National University of Singapore, 18 Science Drive 4, Singapore 117559, Singapore
4. The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China

✉ Corresponding author: Prof. Kevin Tan. Email: mictankedu.sg

Citation:
Deng L, Wojciech L, Png CW, Kioh DYQ, Gu Y, Aung TT, Malleret B, Chan ECY, Peng G, Zhang Y, Gascoigne NRJ, Tan KSW. Colonization with two different Blastocystis subtypes in DSS-induced colitis mice is associated with strikingly different microbiome and pathological features. Theranostics 2023; 13(3):1165-1179. doi:10.7150/thno.81583. https://www.thno.org/v13p1165.htm
Other styles

File import instruction

Abstract

Graphic abstract

Rationale: The gut microbiota plays a significant role in the pathogenesis of inflammatory bowel disease (IBD). However, the role of Blastocystis infection and Blastocystis-altered gut microbiota in the development of inflammatory diseases and their underlying mechanisms are not well understood.

Methods: We investigated the effect of Blastocystis ST4 and ST7 infection on the intestinal microbiota, metabolism, and host immune responses, and then explored the role of Blastocystis-altered gut microbiome in the development of dextran sulfate sodium (DSS)-induced colitis in mice.

Results: This study showed that prior colonization with ST4 conferred protection from DSS-induced colitis through elevating the abundance of beneficial bacteria, short-chain fatty acid (SCFA) production and the proportion of Foxp3+ and IL-10-producing CD4+ T cells. Conversely, prior ST7 infection exacerbated the severity of colitis by increasing the proportion of pathogenic bacteria and inducing pro-inflammatory IL-17A and TNF-α-producing CD4+ T cells. Furthermore, transplantation of ST4- and ST7-altered microbiota resulted in similar phenotypes.

Conclusions: Our data showed that ST4 and ST7 infection exert strikingly differential effects on the gut microbiota, and these could influence the susceptibility to colitis. ST4 colonization prevented DSS-induced colitis in mice and may be considered as a novel therapeutic strategy against immunological diseases in the future, while ST7 infection is a potential risk factor for the development of experimentally induced colitis that warrants attention.

Keywords: Blastocystis, Gut microbiota, IBD, DSS-induced colitis, Short-chain fatty acids

Citation styles

APA
Deng, L., Wojciech, L., Png, C.W., Kioh, D.Y.Q., Gu, Y., Aung, T.T., Malleret, B., Chan, E.C.Y., Peng, G., Zhang, Y., Gascoigne, N.R.J., Tan, K.S.W. (2023). Colonization with two different Blastocystis subtypes in DSS-induced colitis mice is associated with strikingly different microbiome and pathological features. Theranostics, 13(3), 1165-1179. https://doi.org/10.7150/thno.81583.

ACS
Deng, L.; Wojciech, L.; Png, C.W.; Kioh, D.Y.Q.; Gu, Y.; Aung, T.T.; Malleret, B.; Chan, E.C.Y.; Peng, G.; Zhang, Y.; Gascoigne, N.R.J.; Tan, K.S.W. Colonization with two different Blastocystis subtypes in DSS-induced colitis mice is associated with strikingly different microbiome and pathological features. Theranostics 2023, 13 (3), 1165-1179. DOI: 10.7150/thno.81583.

NLM
Deng L, Wojciech L, Png CW, Kioh DYQ, Gu Y, Aung TT, Malleret B, Chan ECY, Peng G, Zhang Y, Gascoigne NRJ, Tan KSW. Colonization with two different Blastocystis subtypes in DSS-induced colitis mice is associated with strikingly different microbiome and pathological features. Theranostics 2023; 13(3):1165-1179. doi:10.7150/thno.81583. https://www.thno.org/v13p1165.htm

CSE
Deng L, Wojciech L, Png CW, Kioh DYQ, Gu Y, Aung TT, Malleret B, Chan ECY, Peng G, Zhang Y, Gascoigne NRJ, Tan KSW. 2023. Colonization with two different Blastocystis subtypes in DSS-induced colitis mice is associated with strikingly different microbiome and pathological features. Theranostics. 13(3):1165-1179.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Popup Image

©2024 Ivyspring International Publisher. Terms of use