Theranostics 2012; 2(3):251-257. doi:10.7150/thno.3726
Carbon Nanotube Thin Film Biosensors for Sensitive and Reproducible Whole Virus Detection
Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.
Mandal HS, Su Z, Ward A, Tang XS. Carbon Nanotube Thin Film Biosensors for Sensitive and Reproducible Whole Virus Detection. Theranostics 2012; 2(3):251-257. doi:10.7150/thno.3726. Available from http://www.thno.org/v02p0251.htm
Here, we report the label-free, sensitive, and real-time electrical detection of whole viruses using carbon nanotube thin film (CNT-TF) field effect devices. Selective detection of approximately 550 model viruses, M13-bacteriophage, is demonstrated using a simple two-terminal (no gate electrode) configuration. Chemical gating through specific antibody-virus binding on CNT surface is proposed to be the sensing mechanism. Compared to electrical impedance sensors with identical microelectrode dimensions (no CNT), the CNT-TF sensors exhibit sensitivity 5 orders higher. We believe the reported approach could lead to a reproducible and cost-effective solution for rapid viral identification.
Keywords: carbon nanotube, thin film, virus detection, electrochemical impedance sensor, microelectrodes, M13-pIII antibody, M13 bacteriophage, ELISA.