Theranostics 2016; 6(7):969-985. doi:10.7150/thno.14856 This issue


Click Chemistry-Mediated Nanosensors for Biochemical Assays

Yiping Chen*, Yunlei Xianyu*, Jing Wu, Binfeng Yin, Xingyu Jiang

Beijing Engineering Research Center for BioNanotechnology and CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology, Beijing 100190, China.
*These authors equally contribute to this work.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See for full terms and conditions.
Chen Y, Xianyu Y, Wu J, Yin B, Jiang X. Click Chemistry-Mediated Nanosensors for Biochemical Assays. Theranostics 2016; 6(7):969-985. doi:10.7150/thno.14856. Available from

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Click chemistry combined with functional nanoparticles have drawn increasing attention in biochemical assays because they are promising in developing biosensors with effective signal transformation/amplification and straightforward signal readout for clinical diagnostic assays. In this review, we focus on the latest advances of biochemical assays based on Cu (I)-catalyzed 1, 3-dipolar cycloaddition of azides and alkynes (CuAAC)-mediated nanosensors, as well as the functionalization of nanoprobes based on click chemistry. Nanoprobes including gold nanoparticles, quantum dots, magnetic nanoparticles and carbon nanomaterials are covered. We discuss the advantages of click chemistry-mediated nanosensors for biochemical assays, and give perspectives on the development of click chemistry-mediated approaches for clinical diagnosis and other biomedical applications.

Keywords: Click chemistry, nanosensor, bio-conjugation, signal amplification system.