Full Text | PDF

Share on tweeters Share on facebook Share on linkedin Share on googleplus

Theranostics 2020; 10(26):12060-12071. doi:10.7150/thno.48918 This issue Cite

Review

Circulating tumor cells in hepatocellular carcinoma: single-cell based analysis, preclinical models, and clinical applications

Qian Zhang#, Yuan Rong#, Kezhen Yi, Lanxiang Huang, Ming Chen, Fubing Wang✉

Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, P. R. China.
#Equal contributions to this work.

✉ Corresponding author: Fu-Bing Wang, Department of Laboratory Medicine, Zhongnan Hospital of Wuhan University, No.169 Donghu Road, Wuchang District, Wuhan 430071, P.R. China. Phone: +86-27-67813517; E-mail: wfb20042002com.

Citation:
Zhang Q, Rong Y, Yi K, Huang L, Chen M, Wang F. Circulating tumor cells in hepatocellular carcinoma: single-cell based analysis, preclinical models, and clinical applications. Theranostics 2020; 10(26):12060-12071. doi:10.7150/thno.48918. https://www.thno.org/v10p12060.htm
Other styles

File import instruction

Abstract

Graphic abstract

Circulating tumor cells (CTCs) are shed into the bloodstream from primary tumors and metastatic lesions and provide significant information about tumor progression and metastasis. CTCs contribute to tumor metastasis through the epithelial-to-mesenchymal transition (EMT). CTC clusters and stem-like phenotypes lead to a more aggressive and metastatic potential. CTCs retain the heterogeneity and imitate the nature of corresponding primary tumors. Therefore, it is important to use single-cell based analysis to obtain information on tumor heterogeneity and biology. CTCs are also good candidates for building preclinical models (especially 3D organoid cultures) for drug screening, disease modeling, genome editing, tumor immunity research, and organ-like biobank establishment. In this article, we summarize the current CTC capture technology, dissect the phenotypes associated with CTC metastasis, and review the progress in single-cell based analysis and preclinical modeling of the pattern and kinetics of CTCs. In particular, we discuss the use of CTCs to assess the progression of hepatocellular carcinoma (HCC).

Keywords: circulating tumor cells, single-cell based analysis, preclinical models, hepatocellular carcinoma, clinical application

Citation styles

APA
Zhang, Q., Rong, Y., Yi, K., Huang, L., Chen, M., Wang, F. (2020). Circulating tumor cells in hepatocellular carcinoma: single-cell based analysis, preclinical models, and clinical applications. Theranostics, 10(26), 12060-12071. https://doi.org/10.7150/thno.48918.

ACS
Zhang, Q.; Rong, Y.; Yi, K.; Huang, L.; Chen, M.; Wang, F. Circulating tumor cells in hepatocellular carcinoma: single-cell based analysis, preclinical models, and clinical applications. Theranostics 2020, 10 (26), 12060-12071. DOI: 10.7150/thno.48918.

NLM
Zhang Q, Rong Y, Yi K, Huang L, Chen M, Wang F. Circulating tumor cells in hepatocellular carcinoma: single-cell based analysis, preclinical models, and clinical applications. Theranostics 2020; 10(26):12060-12071. doi:10.7150/thno.48918. https://www.thno.org/v10p12060.htm

CSE
Zhang Q, Rong Y, Yi K, Huang L, Chen M, Wang F. 2020. Circulating tumor cells in hepatocellular carcinoma: single-cell based analysis, preclinical models, and clinical applications. Theranostics. 10(26):12060-12071.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Popup Image

©2024 Ivyspring International Publisher. Terms of use