Theranostics 2021; 11(11):5197-5213. doi:10.7150/thno.58433 This issue Cite

Research Paper

Single-cell transcriptomic profiling provides insights into the toxic effects of Zearalenone exposure on primordial follicle assembly

Yu Tian1, Ming-Yu Zhang1, Ai-Hong Zhao2, Li Kong1, Jun-Jie Wang1, Wei Shen1, Lan Li1✉

1. College of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao 266109, China.
2. Qingdao Academy of Agricultural Sciences, Qingdao 266100, China.

Citation:
Tian Y, Zhang MY, Zhao AH, Kong L, Wang JJ, Shen W, Li L. Single-cell transcriptomic profiling provides insights into the toxic effects of Zearalenone exposure on primordial follicle assembly. Theranostics 2021; 11(11):5197-5213. doi:10.7150/thno.58433. https://www.thno.org/v11p5197.htm
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Abstract

Graphic abstract

Rationale: Zearalenone (ZEN), a pollutant in our daily diet, seriously threatens the reproductive health of humans and animals. The primordial follicle (PF) assembly in the mouse occurs during the perinatal period, which determines the whole ovarian reserve in reproductive lifespan. In the current investigation, we administered ZEN orally to perinatal mice from 16.5 days post coitum (dpc) to postnatal day 3 (PD3), and single-cell RNA sequencing (scRNA-seq) was performed on PD0 and PD3 ovarian tissues in the offspring to check ZEN toxic to primordial follicle formation at the single cell level.

Methods: Ovarian tissues (in vivo) were examined by single cell RNA sequencing analysis, Immunostaining, and Western blotting. Ovarian tissues (in vitro) were examined by qRT-PCR, Immunostaining, and Western blotting.

Results: We found that ZEN exposure altered the developmental trajectory of both germ cells and granulosa cells. Furthermore, after establishing the cell-cell communication network between germ cells and granulosa cells, we found that this was perturbed by ZEN exposure, especially during the Hippo signaling pathway.

Conclusions: This study showed that ZEN affected the status of germ cells and granulosa cells through the Hippo signaling pathway and blocked the assembly of PFs. This research contributes to our deeper understanding of the mechanisms of toxicity in different cell types and the disruption of normal intercellular signaling by ZEN exposure.

Keywords: Zearalenone, single-cell RNA-seq, primordial follicle assembly, oocytes, granulosa cells


Citation styles

APA
Tian, Y., Zhang, M.Y., Zhao, A.H., Kong, L., Wang, J.J., Shen, W., Li, L. (2021). Single-cell transcriptomic profiling provides insights into the toxic effects of Zearalenone exposure on primordial follicle assembly. Theranostics, 11(11), 5197-5213. https://doi.org/10.7150/thno.58433.

ACS
Tian, Y.; Zhang, M.Y.; Zhao, A.H.; Kong, L.; Wang, J.J.; Shen, W.; Li, L. Single-cell transcriptomic profiling provides insights into the toxic effects of Zearalenone exposure on primordial follicle assembly. Theranostics 2021, 11 (11), 5197-5213. DOI: 10.7150/thno.58433.

NLM
Tian Y, Zhang MY, Zhao AH, Kong L, Wang JJ, Shen W, Li L. Single-cell transcriptomic profiling provides insights into the toxic effects of Zearalenone exposure on primordial follicle assembly. Theranostics 2021; 11(11):5197-5213. doi:10.7150/thno.58433. https://www.thno.org/v11p5197.htm

CSE
Tian Y, Zhang MY, Zhao AH, Kong L, Wang JJ, Shen W, Li L. 2021. Single-cell transcriptomic profiling provides insights into the toxic effects of Zearalenone exposure on primordial follicle assembly. Theranostics. 11(11):5197-5213.

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