Theranostics 2023; 13(5):1632-1648. doi:10.7150/thno.81718 This issue Cite

Research Paper

Light-triggered photosynthetic engineered bacteria for enhanced-photodynamic therapy by relieving tumor hypoxic microenvironment

Chenyang Yin1*, ZeKun Wang1*, Chunxue Dai1, Bangjia Yang1, Weiyun Wang1, Endong Yang1, Feng Guo1, Cundong Fan2✉, Pu Zhang3✉, Jikui Sun4✉, Dongdong Sun1✉

1. School of Life Sciences, Anhui Agricultural University, Hefei 230036, China.
2. Shandong Key Laboratory of TCM Multi-Target Intervention and Disease Control, the Second Affiliated Hospital of Shandong First Medical University, Taian, Shandong 271000, China.
3. Department of Cardiology, Affiliated Taian City Central Hospital of Qingdao University, Taian, Shandong, 271000, China.
4. Department of Neurosurgery, the First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China.
*These authors contributed equally to this work.

Citation:
Yin C, Wang Z, Dai C, Yang B, Wang W, Yang E, Guo F, Fan C, Zhang P, Sun J, Sun D. Light-triggered photosynthetic engineered bacteria for enhanced-photodynamic therapy by relieving tumor hypoxic microenvironment. Theranostics 2023; 13(5):1632-1648. doi:10.7150/thno.81718. https://www.thno.org/v13p1632.htm
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Abstract

Graphic abstract

Background: Singlet oxygen (1O2) has received considerable research attention in photodynamic therapy (PDT) due to its cytotoxic solid features. However, the inherent hypoxic state of the tumor microenvironment (TME) leads to the meager 1O2 quantum yield of inorganic PDT reagents, and their application in vivo remains elusive.

Methods: We developed a novel strategy to fabricate active photosynthetic bacteria/photosensitizer/photothermal agent hybrids for photosynthetic tumor oxygenation and PDT and PTT tumor therapy under different laser irradiation sources. Photosynthetic bacteria combined with Ce6 photosensitizer and Au NPs photothermal agent, the obtained Bac@Au-Ce6 effectively targets tumor tissues and further enhances the tumor accumulation of Au-Ce6.

Results: The results showed that the Au-Ce6-loaded engineered bacteria (Bac@Au-Ce6) maintained the photosynthetic properties of Syne. After i.v. injection, Bac@Au-Ce6 efficiently aggregates at tumor sites due to the tumor-targeting ability of active Syne. With 660 nm laser irradiation at the tumor site, the photoautotrophic Syne undergoes sustained photosynthetic O2 release and immediately activates O2 to 1O2 via a loaded photosensitizer. PTT was subsequently imparted by 808 laser irradiations to enhance tumor killing further.

Conclusions: This work provides a new platform for engineering bacteria-mediated photosynthesis to promote PDT combined with PTT multi-faceted anti-tumor.

Keywords: singlet oxygen, photodynamic therapy, tumor hypoxic microenvironment, photosynthetic bacteria, photothermal therapy.


Citation styles

APA
Yin, C., Wang, Z., Dai, C., Yang, B., Wang, W., Yang, E., Guo, F., Fan, C., Zhang, P., Sun, J., Sun, D. (2023). Light-triggered photosynthetic engineered bacteria for enhanced-photodynamic therapy by relieving tumor hypoxic microenvironment. Theranostics, 13(5), 1632-1648. https://doi.org/10.7150/thno.81718.

ACS
Yin, C.; Wang, Z.; Dai, C.; Yang, B.; Wang, W.; Yang, E.; Guo, F.; Fan, C.; Zhang, P.; Sun, J.; Sun, D. Light-triggered photosynthetic engineered bacteria for enhanced-photodynamic therapy by relieving tumor hypoxic microenvironment. Theranostics 2023, 13 (5), 1632-1648. DOI: 10.7150/thno.81718.

NLM
Yin C, Wang Z, Dai C, Yang B, Wang W, Yang E, Guo F, Fan C, Zhang P, Sun J, Sun D. Light-triggered photosynthetic engineered bacteria for enhanced-photodynamic therapy by relieving tumor hypoxic microenvironment. Theranostics 2023; 13(5):1632-1648. doi:10.7150/thno.81718. https://www.thno.org/v13p1632.htm

CSE
Yin C, Wang Z, Dai C, Yang B, Wang W, Yang E, Guo F, Fan C, Zhang P, Sun J, Sun D. 2023. Light-triggered photosynthetic engineered bacteria for enhanced-photodynamic therapy by relieving tumor hypoxic microenvironment. Theranostics. 13(5):1632-1648.

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