Theranostics 2017; 7(10):2620-2633. doi:10.7150/thno.18096
A High-Affinity Repebody for Molecular Imaging of EGFR-Expressing Malignant Tumors
1. Department of Nuclear Medicine, Chonnam National University Medical School and Hwasun Hospital, Jeonnam, Republic of Korea
2. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea
3. Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon, Republic of Korea
4. Department of Forensic Medicine, Chonnam National University Medical School, Gwangju, Republic of Korea
5. Department of Molecular Medicine (BK21plus), Chonnam National University Graduate School, Gwangju, Republic of Korea
6. Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju, Republic of Korea
7. School of Mechanical Engineering, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea
8. Department of Microbiology, Chonnam National University Medical School, Gwangju, Republic of Korea
9. Department of Radiology, Chonnam National University Hwasun Hospital, Jeonnam, Republic of Korea
10. Department of Biology, Kyung Hee University College of Science, Seoul, Republic of Korea
11. Division of RI-Convergence Research, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea
12. Department of Gastroenterology, Chonnam National University Hwasun Hospital, Jeonnam, Republic of Korea
* These authors contributed equally to this research.
Yun M, Kim DY, Lee Jj, Kim HS, Kim HS, Pyo A, Ryu Y, Kim TY, Zheng JH, Yoo SW, Hyun H, Oh G, Jeong J, Moon M, Min JH, Kwon SY, Kim JY, Chung E, Hong Y, Lee W, Kim HS, Min JJ. A High-Affinity Repebody for Molecular Imaging of EGFR-Expressing Malignant Tumors. Theranostics 2017; 7(10):2620-2633. doi:10.7150/thno.18096. Available from https://www.thno.org/v07p2620.htm
The accurate detection of disease-related biomarkers is crucial for the early diagnosis and management of disease in personalized medicine. Here, we present a molecular imaging of human epidermal growth factor receptor (EGFR)-expressing malignant tumors using an EGFR-specific repebody composed of leucine-rich repeat (LRR) modules. The repebody was labeled with either a fluorescent dye or radioisotope, and used for imaging of EGFR-expressing malignant tumors using an optical method and positron emission tomography. Our approach enabled visualization of the status of EGFR expression, allowing quantitative evaluation in whole tumors, which correlated well with the EGFR expression levels in mouse or patients-derived colon cancers. The present approach can be effectively used for the accurate detection of EGFR-expressing cancers, assisting in the development of a tool for detecting other disease biomarkers.
Keywords: Repebody, Epidermal growth factor receptor, Colon cancer, Molecular imaging, PET, Optical imaging.