Theranostics 2021; 11(11):5160-5173. doi:10.7150/thno.54864 This issue Cite

Research Paper

3D-bioprinting ready-to-implant anisotropic menisci recapitulate healthy meniscus phenotype and prevent secondary joint degeneration

Ye Sun1,2,3✉#, Yuxin Zhang4#, Qiang Wu2#, Feng Gao1#, Yongzhong Wei1#, Yimin Ma1, Wenbo Jiang2, Kerong Dai2,3

1. Department of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, 210029, China.
2. Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
3. Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
4. Department of Rehabilitation Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
#These authors contributed equally to the article.

Citation:
Sun Y, Zhang Y, Wu Q, Gao F, Wei Y, Ma Y, Jiang W, Dai K. 3D-bioprinting ready-to-implant anisotropic menisci recapitulate healthy meniscus phenotype and prevent secondary joint degeneration. Theranostics 2021; 11(11):5160-5173. doi:10.7150/thno.54864. https://www.thno.org/v11p5160.htm
Other styles

File import instruction

Abstract

Graphic abstract

Objectives: Disruption of anisotropic phenotypes of the meniscus would contribute to OA progression. Exploring phenotype changes of the anisotropic meniscus in joint degeneration would help understand the biologic interaction between the meniscus and OA, and further facilitate the therapeutic strategies of meniscus injury-related joint degeneration. Meanwhile, engineering biomimetic meniscal tissue mimicking the anisotropy of the healthy meniscus remains a challenge.

Methods & Results: Meniscal disruption of phenotype anisotropy (PBV growth, cellular phenotype and ECM depositions) was confirmed in OA patient samples. To recapitulate healthy meniscus phenotypes, 3D-bioprinted anisotropic TCM meniscus constructs with PBV growth and regional differential cell and ECM depositions were generated. Transplanted 3D-bioprinted meniscus into rabbit knees recapitulated phenotypes of native healthy meniscus and conferred long-term protection against secondary joint degeneration.

Conclusion: 3D-bioprinted TCM meniscus not only restored the anisotropy of native healthy meniscus with PBV infiltration and better shape retention, but better maintained joint function and prevented secondary joint degeneration, which provided a new strategy for the clinical treatment of meniscus injury-related joint degenerative diseases.

Keywords: meniscus, 3d-print, bio-print, hydrogel, tissue engineering, chondrocyte, vascularization


Citation styles

APA
Sun, Y., Zhang, Y., Wu, Q., Gao, F., Wei, Y., Ma, Y., Jiang, W., Dai, K. (2021). 3D-bioprinting ready-to-implant anisotropic menisci recapitulate healthy meniscus phenotype and prevent secondary joint degeneration. Theranostics, 11(11), 5160-5173. https://doi.org/10.7150/thno.54864.

ACS
Sun, Y.; Zhang, Y.; Wu, Q.; Gao, F.; Wei, Y.; Ma, Y.; Jiang, W.; Dai, K. 3D-bioprinting ready-to-implant anisotropic menisci recapitulate healthy meniscus phenotype and prevent secondary joint degeneration. Theranostics 2021, 11 (11), 5160-5173. DOI: 10.7150/thno.54864.

NLM
Sun Y, Zhang Y, Wu Q, Gao F, Wei Y, Ma Y, Jiang W, Dai K. 3D-bioprinting ready-to-implant anisotropic menisci recapitulate healthy meniscus phenotype and prevent secondary joint degeneration. Theranostics 2021; 11(11):5160-5173. doi:10.7150/thno.54864. https://www.thno.org/v11p5160.htm

CSE
Sun Y, Zhang Y, Wu Q, Gao F, Wei Y, Ma Y, Jiang W, Dai K. 2021. 3D-bioprinting ready-to-implant anisotropic menisci recapitulate healthy meniscus phenotype and prevent secondary joint degeneration. Theranostics. 11(11):5160-5173.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Popup Image