Theranostics 2016; 6(10):1732-1739. doi:10.7150/thno.16129 This issue Cite

Research Paper

Metal-linked Immunosorbent Assay (MeLISA): the Enzyme-Free Alternative to ELISA for Biomarker Detection in Serum

Ru-Jia Yu1†, Wei Ma1†, Xiao-Yuan Liu1, Hong-Ying Jin1, Huan-Xing Han2, Hong-Yang Wang3, He Tian1✉, Yi-Tao Long1✉

1. Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, P. R. China.
2. Translational Medicine Center, Changzheng Hospital, The Second Military Medical University, P. R. China.
3. Laboratory of Signal Transduction, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, P. R. China.
†These authors contributed equally.

Citation:
Yu RJ, Ma W, Liu XY, Jin HY, Han HX, Wang HY, Tian H, Long YT. Metal-linked Immunosorbent Assay (MeLISA): the Enzyme-Free Alternative to ELISA for Biomarker Detection in Serum. Theranostics 2016; 6(10):1732-1739. doi:10.7150/thno.16129. https://www.thno.org/v06p1732.htm
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Abstract

Graphic abstract

Determination of disease biomarkers in clinical samples is of crucial significance for disease monitoring and public health. The dominating format is enzyme-linked immunosorbent assay (ELISA), which subtly exploits both the antigen-antibody reaction and biocatalytic property of enzymes. Although enzymes play an important role in this platform, they generally suffer from inferior stability and less tolerant of temperature, pH condition compared with general chemical product. Here, we demonstrate a metal-linked immunosorbent assay (MeLISA) based on a robust signal amplification mechanism that faithfully replaces the essential element of the enzyme. As an enzyme-free alternative to ELISA, this methodology works by the detection of α-fetoprotein (AFP), prostatic specific antigen (PSA) and C-reactive protein (CRP) at concentrations of 0.1 ng mL-1, 0.1 ng mL-1 and 1 ng mL-1 respectively. It exhibits approximately two magnitudes higher sensitivity and is 4 times faster for chromogenic reaction than ELISA. The detection of AFP and PSA was further confirmed by over a hundred serum samples from hepatocellular carcinoma (HCC) and prostate cancer patients respectively.

Keywords: MeLISA


Citation styles

APA
Yu, R.J., Ma, W., Liu, X.Y., Jin, H.Y., Han, H.X., Wang, H.Y., Tian, H., Long, Y.T. (2016). Metal-linked Immunosorbent Assay (MeLISA): the Enzyme-Free Alternative to ELISA for Biomarker Detection in Serum. Theranostics, 6(10), 1732-1739. https://doi.org/10.7150/thno.16129.

ACS
Yu, R.J.; Ma, W.; Liu, X.Y.; Jin, H.Y.; Han, H.X.; Wang, H.Y.; Tian, H.; Long, Y.T. Metal-linked Immunosorbent Assay (MeLISA): the Enzyme-Free Alternative to ELISA for Biomarker Detection in Serum. Theranostics 2016, 6 (10), 1732-1739. DOI: 10.7150/thno.16129.

NLM
Yu RJ, Ma W, Liu XY, Jin HY, Han HX, Wang HY, Tian H, Long YT. Metal-linked Immunosorbent Assay (MeLISA): the Enzyme-Free Alternative to ELISA for Biomarker Detection in Serum. Theranostics 2016; 6(10):1732-1739. doi:10.7150/thno.16129. https://www.thno.org/v06p1732.htm

CSE
Yu RJ, Ma W, Liu XY, Jin HY, Han HX, Wang HY, Tian H, Long YT. 2016. Metal-linked Immunosorbent Assay (MeLISA): the Enzyme-Free Alternative to ELISA for Biomarker Detection in Serum. Theranostics. 6(10):1732-1739.

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