Full Text | PDF

Share on tweeters Share on facebook Share on linkedin Share on googleplus

Theranostics 2021; 11(9):4078-4089. doi:10.7150/thno.52574 This issue Cite

Research Paper

Electroacupuncture ameliorates intestinal inflammation by activating α7nAChR-mediated JAK2/STAT3 signaling pathway in postoperative ileus

Na-Na Yang1, Jing-Wen Yang1, Yang Ye2, Jin Huang1, Lu Wang1, Yu Wang1, Xin-Tong Su1, Ying Lin1, Fang-Ting Yu1, Si-Ming Ma1, Ling-Yu Qi1, Lu-Lu Lin1, Li-Qiong Wang1, Guang-Xia Shi1, Hong-Ping Li1, Cun-Zhi Liu1✉

1. International Acupuncture and Moxibustion Innovation Institute, Beijing University of Chinese Medicine.
2. Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China.

✉ Corresponding author: Cun-Zhi Liu, PhD, International Acupuncture and Moxibustion Innovation Institute, Beijing University of Chinese Medicine, No. 11 Bei San Huan Dong Lu, Chaoyang District, Beijing 100029, China. E-mail: lcz623780 com.

Citation:
Yang NN, Yang JW, Ye Y, Huang J, Wang L, Wang Y, Su XT, Lin Y, Yu FT, Ma SM, Qi LY, Lin LL, Wang LQ, Shi GX, Li HP, Liu CZ. Electroacupuncture ameliorates intestinal inflammation by activating α7nAChR-mediated JAK2/STAT3 signaling pathway in postoperative ileus. Theranostics 2021; 11(9):4078-4089. doi:10.7150/thno.52574. https://www.thno.org/v11p4078.htm
Other styles

File import instruction

Abstract

Graphic abstract

Inflammatory cytokines produced by muscularis macrophages largely contribute to the pathological signs of postoperative ileus (POI). Electroacupuncture (EA) can suppress inflammation, mainly or partly via activation of vagal efferent. The goal of this study was to investigate the mechanisms by which EA stimulation at an hindlimb region ameliorates inflammation in POI.

Methods: Intestinal motility and inflammation were examined after 24 h after intestinal manipulation (IM)-induced POI in mice. Local immune response in the intestinal muscularis, expression of macrophages, α7 nicotinic acetylcholine receptor (α7nAChR), Janus kinase 2 (JAK2) and signal transducer and activator of transcription 3 (STAT3) were determined by flow cytometry, Western Blot, qPCR and immunofluorescence. The effects of α7nAChR antagonists (methyllycaconitine and α-bungarotoxin) and JAK2/STAT3 inhibitors (AG490 and WP1066) were also administered in a subset of mice prior to EA. In the parasympathetic pathways, intestinal motility and inflammation were determined after cervical vagotomy and sub-diaphragmatic vagotomy. The expression of gamma absorptiometry aminobutyric acid (GABAA) receptor in dorsal motor nucleus of vagal (DMV) cholinergic neurons was assessed by immunofluorescence and the response to DMV microinjection of bicuculine (antagonist of GABAA receptor) or muscimol (agonist of GABAA receptor) were assessed.

Results: EA suppressed intestinal inflammation and promoted gastrointestinal motility. Mechanistically, EA activated the α7nAChR-mediated JAK2/STAT3 signaling pathway in macrophages which reduced the production of inflammatory cytokines. Furthermore, we also demonstrated that hindlimb region stimulation drove vagal efferent output by inhibiting the expression of GABAA receptor in DMV to ameliorate inflammation.

Conclusions: The present study revealed that EA of hindlimb regions inhibited the expression of GABAA receptor in DMV neurons, whose excited vagal nerve, in turn suppressed IM-induced inflammation via activation of α7nAChR-mediated JAK2/STAT3 signaling pathway.

Keywords: gastrointestinal motility, macrophages, α7nAChR, JAK2/STAT3 signaling pathway, GABAA receptor

Citation styles

APA
Yang, N.N., Yang, J.W., Ye, Y., Huang, J., Wang, L., Wang, Y., Su, X.T., Lin, Y., Yu, F.T., Ma, S.M., Qi, L.Y., Lin, L.L., Wang, L.Q., Shi, G.X., Li, H.P., Liu, C.Z. (2021). Electroacupuncture ameliorates intestinal inflammation by activating α7nAChR-mediated JAK2/STAT3 signaling pathway in postoperative ileus. Theranostics, 11(9), 4078-4089. https://doi.org/10.7150/thno.52574.

ACS
Yang, N.N.; Yang, J.W.; Ye, Y.; Huang, J.; Wang, L.; Wang, Y.; Su, X.T.; Lin, Y.; Yu, F.T.; Ma, S.M.; Qi, L.Y.; Lin, L.L.; Wang, L.Q.; Shi, G.X.; Li, H.P.; Liu, C.Z. Electroacupuncture ameliorates intestinal inflammation by activating α7nAChR-mediated JAK2/STAT3 signaling pathway in postoperative ileus. Theranostics 2021, 11 (9), 4078-4089. DOI: 10.7150/thno.52574.

NLM
Yang NN, Yang JW, Ye Y, Huang J, Wang L, Wang Y, Su XT, Lin Y, Yu FT, Ma SM, Qi LY, Lin LL, Wang LQ, Shi GX, Li HP, Liu CZ. Electroacupuncture ameliorates intestinal inflammation by activating α7nAChR-mediated JAK2/STAT3 signaling pathway in postoperative ileus. Theranostics 2021; 11(9):4078-4089. doi:10.7150/thno.52574. https://www.thno.org/v11p4078.htm

CSE
Yang NN, Yang JW, Ye Y, Huang J, Wang L, Wang Y, Su XT, Lin Y, Yu FT, Ma SM, Qi LY, Lin LL, Wang LQ, Shi GX, Li HP, Liu CZ. 2021. Electroacupuncture ameliorates intestinal inflammation by activating α7nAChR-mediated JAK2/STAT3 signaling pathway in postoperative ileus. Theranostics. 11(9):4078-4089.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Popup Image

©2024 Ivyspring International Publisher. Terms of use