Theranostics 2021; 11(20):9821-9832. doi:10.7150/thno.62187 This issue Cite

Research Paper

CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis

Xiaoxun Li1,2*, Yajun Liang2,*, Cheng Lian2,*, Fangli Peng2, Yansen Xiao2, Yunfei He2, Chengxin Ma2, Yuan Wang2, Peiyuan Zhang2, Yuhan Deng1, Yunpeng Su4, Cheng Luo5, Xiangyin Kong2, Qingcheng Yang3, Tong Liu1,✉, Guohong Hu2,3✉

1. Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, China.
2. CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
3. Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
4. Novomab Biopharmaceuticals Inc., Nanjing, China.
5. Drug Discovery and Design Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
*These authors contributed equally to this work.

Citation:
Li X, Liang Y, Lian C, Peng F, Xiao Y, He Y, Ma C, Wang Y, Zhang P, Deng Y, Su Y, Luo C, Kong X, Yang Q, Liu T, Hu G. CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis. Theranostics 2021; 11(20):9821-9832. doi:10.7150/thno.62187. https://www.thno.org/v11p9821.htm
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Abstract

Graphic abstract

Background: Bone metastasis is a frequent symptom of breast cancer and current targeted therapy has limited efficacy. Osteoclasts play critical roles to drive osteolysis and metastatic outgrowth of tumor cells in bone. Previously we identified CST6 as a secretory protein significantly downregulated in bone-metastatic breast cancer cells. Functional analysis showed that CST6 suppresses breast-to-bone metastasis in animal models. However, the functional mechanism and therapeutic potential of CST6 in bone metastasis is unknown.

Methods: Using in vitro osteoclastogenesis and in vivo metastasis assays, we studied the effect and mechanism of extracellular CST6 protein in suppressing osteoclastic niches and bone metastasis of breast cancer. A number of peptides containing the functional domain of CST6 were screened to inhibit bone metastasis. The efficacy, stability and toxicity of CST6 recombinant protein and peptides were evaluated in preclinical metastasis models.

Results: We show here that CST6 inhibits osteolytic bone metastasis by inhibiting osteoclastogenesis. Cancer cell-derived CST6 enters osteoclasts by endocytosis and suppresses the cysteine protease CTSB, leading to up-regulation of the CTSB hydrolytic substrate SPHK1. SPHK1 suppresses osteoclast maturation by inhibiting the RANKL-induced p38 activation. Importantly, recombinant CST6 protein effectively suppresses bone metastasis in vitro and in vivo. We further identified several peptides mimicking the function of CST6 to suppress cancer cell-induced osteoclastogenesis and bone metastasis. Pre-clinical analyses of CTS6 recombinant protein and peptides demonstrated their potentials in treatment of breast cancer bone metastasis.

Conclusion: These findings reveal the CST6-CTSB-SPHK1 signaling axis in osteoclast differentiation and provide a promising approach to treat bone diseases with CST6-based peptides.

Keywords: Breast cancer, Bone metastasis, Osteoclastogenesis, CST6, Peptide drug


Citation styles

APA
Li, X., Liang, Y., Lian, C., Peng, F., Xiao, Y., He, Y., Ma, C., Wang, Y., Zhang, P., Deng, Y., Su, Y., Luo, C., Kong, X., Yang, Q., Liu, T., Hu, G. (2021). CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis. Theranostics, 11(20), 9821-9832. https://doi.org/10.7150/thno.62187.

ACS
Li, X.; Liang, Y.; Lian, C.; Peng, F.; Xiao, Y.; He, Y.; Ma, C.; Wang, Y.; Zhang, P.; Deng, Y.; Su, Y.; Luo, C.; Kong, X.; Yang, Q.; Liu, T.; Hu, G. CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis. Theranostics 2021, 11 (20), 9821-9832. DOI: 10.7150/thno.62187.

NLM
Li X, Liang Y, Lian C, Peng F, Xiao Y, He Y, Ma C, Wang Y, Zhang P, Deng Y, Su Y, Luo C, Kong X, Yang Q, Liu T, Hu G. CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis. Theranostics 2021; 11(20):9821-9832. doi:10.7150/thno.62187. https://www.thno.org/v11p9821.htm

CSE
Li X, Liang Y, Lian C, Peng F, Xiao Y, He Y, Ma C, Wang Y, Zhang P, Deng Y, Su Y, Luo C, Kong X, Yang Q, Liu T, Hu G. 2021. CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis. Theranostics. 11(20):9821-9832.

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